HSC70 ELISA Kit

Mfr.No.	SKT-106
Alternative Names	HSC54 ELISA Kit, HSC71 ELISA Kit, HSC73 ELISA Kit, HSP71 ELISA Kit, HSP73 ELISA Kit, HSPA10 ELISA Kit, HSPA8 ELISA Kit, LAP1 ELISA Kit, NIP71 ELISA Kit, HSC54 ELISA Kit ; HSC71 ELISA Kit ; HSC73 ELISA Kit ; HSP71 ELISA Kit ; HSP73 ELISA Kit ; HSPA10 ELISA Kit ; HSPA8 ELISA Kit ; LAP1 ELISA Kit ; NIP71 ELISA Kit
Categories	ELISA Kits
Description	Colorimetric detection of HSC70
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Gross Weight (kg)	2
Net Weight (g)	500
Reactivity	Human
Scientific Background	HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity (2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). When cells are subjected to metabolic stress (e.g., heat shock) a member of the HSP 70 family, HSP 70 (HSP72), is expressed; HSP 70 is highly related to HSC70 (>90% sequence identity). Constitutively expressed HSC70 rapidly forms a stable complex with the highly inducible HSP70 in cells following heat shock. The interaction of HSC70 with HSP 70 is regulated by ATP. These two heat shock proteins move together in the cell experiencing stress. Furthermore, research on HSC70 has implicates it with a role in facilitating the recovery of centrosomal structure and function after heat shock (6).
Size	1 Plate ; 1 vial/10 ml ; 2 vials ; 1 vial/ 50 ml ; 1 vial/100 ml ; 1 vial/150 µl ; 1 vial/ 13 ml ; 1 vial/150 µl ; 1 vial/ 13 ml ; 1 vial/ 13 ml ; 1 vial/ 13 ml
Adr Code	Non-hazardous
Assay Overview	1. Prepare Standard and samples in Standard and Sample Diluent. 2. Add 100 µL of Standard or sample to appropriate wells. 3. Cover plate with Plate Sealer and incubate at room temperature (20-25°C) for 1 hour. 4. Wash plate four times with 1X Wash Buffer. 5. Add 100 µL of Biotinylated Antibody Working Solution to each well. 6. Cover plate with Plate Sealer and incubate at room temperature for 1 hour. 7. Wash plate four times with 1X Wash Buffer. 8. Add 100 µL of Streptavidin-HRP Working Solution to each well. 9. Cover plate with Plate Sealer and incubate at room temperature for 30 minutes. 10. Wash plate four times with 1X Wash Buffer. 11. Add 100 µL of TMB Substrate to each well. 12. Develop the plate in the dark at room temperature for 30 minutes. 13. Stop reaction by adding 100 µL of Stop Solution to each well. 14. Measure absorbance on a plate reader at 450 nm.
Assay Range	2.34 - 150 ng/ml
Assay Type	Sandwich ELISA (Enzyme-linked Immunosorbent Assay)
Assay Type Abbreviation	Sandwich
Component Number	SKC-106A ; SKC-106B ; SKC-106C ; SKC-106D ; SKC-106E ; SKC-106F ; SKC-106G ; SKC-106H ; SKC-106I ; SKC-106J ; SKC-106K
Country Of Origin	Canada
Detection Method	Colorimetric Assay
Detection Range	2.34 - 150 ng/ml
Incubation Time	30 minutes
Item	Anti-Hsc70 Immunoassay Plate ; 5X Hsc70 Extraction Reagent ; Recombinant Hsc70 Standard ; Standard and Sample Diluent ; 10X Wash Buffer Concentrate ; Anti-Hsc70 Biotinylated Antibody Concentrate ; Anti-Hsc70 Biotinylated Antibody Diluent ; Streptavidin: HRP Concentrate ; Streptavidin: HRP Diluent ; TMB Substrate ; Stop Solution
Method Type	Cell Lysates, Tissue, Serum
Number Of Samples	40 samples in duplicate
Platform	Microplate
Sensitivity	1.54 ng/ml
Species Reactivity Abbreviations	Hu
Species Reactivity Medium	Human
Trademark	StressXpress®

Research Area	Cancer Heat Shock
Tarriff Code	3822.00.1090
Unspsc Code	12352203

Shipping Temperature

Blue Ice

Description

Trademark: StressXpress®
Platform: Microplate
Utility: ELISA kit used to quantitate HSC70 concentration in samples.
kit-overview:

Component No.	Item	Quantity / Size
SKC-106A	Anti-Hsc70 Immunoassay Plate	1 Plate
SKC-106B	5X Hsc70 Extraction Reagent	1 vial/10 ml
SKC-106C	Recombinant Hsc70 Standard	2 vials
SKC-106D	Standard and Sample Diluent	1 vial/ 50 ml
SKC-106E	10X Wash Buffer Concentrate	1 vial/100 ml
SKC-106F	Anti-Hsc70 Biotinylated Antibody Concentrate	1 vial/150 µl
SKC-106G	Anti-Hsc70 Biotinylated Antibody Diluent	1 vial/ 13 ml
SKC-106H	Streptavidin: HRP Concentrate	1 vial/150 µl
SKC-106I	Streptavidin: HRP Diluent	1 vial/ 13 ml
SKC-106J	TMB Substrate	1 vial/ 13 ml
SKC-106K	Stop Solution	1 vial/ 13 ml

Incubation Time: 30 minutes
Shipping Temperature: Blue Ice
Gross Weight (kg): 2
Net Weight (g): 500
Stock Product: Y
Country of Origin: Canada

Storage

4ºC

Storage Temperature

4ºC

Stock Product

Utility

ELISA kit used to quantitate HSC70 concentration in samples.

Reference	1. Brown C.L., et al. (1993) J.Cell Biol., 120 (5): 1101-1112. 2. Boorstein W.R., Ziegelhoffer T., and Craig E.A. (1993) J. Mol. Evol. 38(1): 1-17. 3. Rothman J. (1989) Cell 59: 591-601. 4. DeLuca-Flaherty, et al. (1990) Cell 62: 875-887. 5. Bork P., Sander C., and Valencia A. (1992) Proc. Natl Acad. Sci. USA 89: 7290-7294. 6. Brown C.L., et al. (1996) J. Biol. Chem. 271(2): 833-840.

Protocols Description	1. Prepare Standard and samples in Standard and Sample Diluent. 2. Add 100 µL of Standard or sample to appropriate wells. 3. Cover plate with Plate Sealer and incubate at room temperature (20-25°C) for 1 hour. 4. Wash plate four times with 1X Wash Buffer. 5. Add 100 µL of Biotinylated Antibody Working Solution to each well. 6. Cover plate with Plate Sealer and incubate at room temperature for 1 hour. 7. Wash plate four times with 1X Wash Buffer. 8. Add 100 µL of Streptavidin-HRP Working Solution to each well. 9. Cover plate with Plate Sealer and incubate at room temperature for 30 minutes. 10. Wash plate four times with 1X Wash Buffer. 11. Add 100 µL of TMB Substrate to each well. 12. Develop the plate in the dark at room temperature for 30 minutes. 13. Stop reaction by adding 100 µL of Stop Solution to each well. 14. Measure absorbance on a plate reader at 450 nm.
Other Resources 1	Kit Booklet Lot No. 131007
Other Resources 2	MSDS

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHE11900003-1	SKT-106-480	5 X 96 well	$3071		Add to Cart
BHE11900003-2	SKT-106-96	96 well	$723		Add to Cart

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