HO-1 ELISA Kit

Mfr.No.	SKT-111
Alternative Names	Heme oxygenase 1 ELISA Kit, Hemox ELISA Kit, HMOX1 ELISA Kit, HO1 ELISA Kit, HO 1 ELISA Kit, HSP32 ELISA Kit, Heme oxygenase 1 ELISA Kit ; Hemox ELISA Kit ; HMOX1 ELISA Kit ; HO1 ELISA Kit ; HO 1 ELISA Kit ; HSP32 ELISA Kit
Categories	ELISA Kits
Description	Colorimetric detection of heme oxygenase 1
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Gross Weight (kg)	2
Net Weight (g)	500
Reactivity	Human, Rat
Scientific Background	Heme-oxygenase is a ubiquitous enzyme that catalyzes the initial and rate-limiting steps in heme catabolism yielding equimolar amounts of biliverdin, iron and carbon monoxide. Biliverdin is subsequently converted to bilirubin and the free iron is sequestered to ferritin (1). These products have important physiological effects as carbon monoxide is a potent vasodilator; biliverdin and bilirubin are potent antioxidants; and the free iron increases oxidative stress and regulates the expression of many mRNAs (2). There are three isoforms of heme-oxygenase, HO-1, HO-2 and HO-3; however HO-1 and HO-2 are the major isoforms as they both have been identified in mammals (3). HO-1, also known as heat shock protein 32, is an inducible isoform activated by most oxidative stress inducers, cytokines, inflammatory agents and heat shock. HO-2 is a constitutive isoform which is expressed under homeostatic conditions. HO-1 is also considered to be a cytoprotective factor in that free heme is highly reactive and cytotoxic, and secondly, carbon monoxide is a mediator inhibiting the inflammatory process and bilirubin is a scavenger for reactive oxygen, both of which are the end products of heme catalyzation (4). It has also been shown that HO-1 deficiency may cause reduced stress defense, a pro-inflammatory tendency (5), susceptibility to atherosclerotic lesion formation (6), endothelial cell injury, and growth retardation (7). Up-regulation of HO-1 is therefore said to be one of the major defense mechanisms of oxidative stress (4).
Size	1 Plate ; 1 vial/10 ml ; 2 vials ; 1 vial/ 50 ml ; 1 vial/100 ml ; 1 vial/150 µl ; 1 vial/ 13 ml ; 1 vial/150 µl ; 1 vial/ 13 ml ; 1 vial/ 13 ml ; 1 vial/ 13 ml ; 1 vial/ 13 ml
Adr Code	Non-hazardous
Assay Overview	1. Prepare Standard and samples in Standard and Sample Diluent. 2. Add 50 µL of Pre-Treatment Buffer to all sample and standard wells. 3. Add 50 µL of Standard and sample to appropriate wells. 4. Cover plate with Plate Sealer and incubate at room temperature (20-25°C) for 2 hours. 5. Wash plate four times with 1X Wash Buffer. 6. Add 100 µL of Biotinylated Antibody Working Solution to each well. 7. Cover plate with Plate Sealer and incubate at room temperature for 1 hour. 8. Wash plate four times with 1X Wash Buffer as described in step 5. 9. Add 100 µL of Streptavidin-HRP Working Solution to each well. 10. Cover plate with Plate Sealer and incubate at room temperature for 30 minutes. 11. Wash plate four times with 1X Wash Buffer as described in step 5. 12. Add 100 µL of TMB Substrate to each well. 13. Develop the plate in the dark at room temperature for 30 minutes. 14. Stop reaction by adding 100 µL of Stop Solution to each well. 15. Measure absorbance on a plate reader at 450 nm.
Assay Range	0.781 - 50 ng/ml
Assay Type	Sandwich ELISA (Enzyme-linked Immunosorbent Assay)
Assay Type Abbreviation	Sandwich
Component Number	SKC-111A ; SKC-111B ; SKC-111C ; SKC-111D ; SKC-111E ; SKC-111F ; SKC-111G ; SKC-111H ; SKC-111I ; SKC-111J ; SKC-111K ; SKC-111L
Country Of Origin	Canada
Detection Method	Colorimetric Assay
Detection Range	0.781 - 50 ng/ml
Incubation Time	30 minutes
Item	Anti-HO-1 Immunoassay Plate ; 5X HO-1 Extraction Reagent ; Recombinant HO-1 Standard ; Standard and Sample Diluent ; 10X Wash Buffer Concentrate ; Anti-HO-1 Biotinylated Antibody Concentrate ; Anti-HO-1 Biotinylated Antibody Diluent ; Streptavidin: HRP Concentrate ; Streptavidin: HRP Diluent ; TMB Substrate ; Stop Solution ; Pre-treatment Buffer
Method Type	Cell Lysates, Tissue, Serum
Number Of Samples	40 samples in duplicate
Platform	Microplate
Sensitivity	0.21 ng/ml
Species Reactivity Abbreviations	Hu ; Rt
Species Reactivity Medium	Human ; Rat
Trademark	StressXpress®

Research Area	Cancer Oxidative Stress
Tarriff Code	3822.00.1090
Unspsc Code	12352203

Shipping Temperature

Blue Ice

Description

Trademark: StressXpress®
Platform: Microplate
Utility: ELISA kit used to quantitate HO-1 concentration in samples.
kit-overview:

Component No.	Item	Quantity / Size
SKC-111A	Anti-HO-1 Immunoassay Plate	1 Plate
SKC-111B	5X HO-1 Extraction Reagent	1 vial/10 ml
SKC-111C	Recombinant HO-1 Standard	2 vials
SKC-111D	Standard and Sample Diluent	1 vial/ 50 ml
SKC-111E	10X Wash Buffer Concentrate	1 vial/100 ml
SKC-111F	Anti-HO-1 Biotinylated Antibody Concentrate	1 vial/150 µl
SKC-111G	Anti-HO-1 Biotinylated Antibody Diluent	1 vial/ 13 ml
SKC-111H	Streptavidin: HRP Concentrate	1 vial/150 µl
SKC-111I	Streptavidin: HRP Diluent	1 vial/ 13 ml
SKC-111J	TMB Substrate	1 vial/ 13 ml
SKC-111K	Stop Solution	1 vial/ 13 ml
SKC-111L	Pre-treatment Buffer	1 vial/ 13 ml

Incubation Time: 30 minutes
Shipping Temperature: Blue Ice
Gross Weight (kg): 2
Net Weight (g): 500
Stock Product: Y
Country of Origin: Canada

Storage

4ºC

Storage Temperature

4ºC

Stock Product

Utility

ELISA kit used to quantitate HO-1 concentration in samples.

Reference	1. Froh M. et al. (2007) World J. Gastroentereol 13(25): 3478-86. 2. Elbirt K.K. and Bonkovsky H.L. (1999) Proc Assoc Am Physicians 111(5): 348-47. 3. Maines M.D., Trakshel G.M., and Kutty R.K. (1986) J Biol Chem 261: 411–419. 4. Brydun A., et al. (2007) Hypertens Res 30(4): 341-8. 5. Poss K.D. and Tonegawa S. (1997). Proc Natl Acad Sci U S A. 94: 10925–10930. 6. Yet S.F., et al. (2003) FASEB J. 17: 1759–1761. 7. Yachie A., et al. (1999) J Clin Invest. 103: 129–135.

Protocols Description	1. Prepare Standard and samples in Standard and Sample Diluent. 2. Add 50 µL of Pre-Treatment Buffer to all sample and standard wells. 3. Add 50 µL of Standard and sample to appropriate wells. 4. Cover plate with Plate Sealer and incubate at room temperature (20-25°C) for 2 hours. 5. Wash plate four times with 1X Wash Buffer. 6. Add 100 µL of Biotinylated Antibody Working Solution to each well. 7. Cover plate with Plate Sealer and incubate at room temperature for 1 hour. 8. Wash plate four times with 1X Wash Buffer as described in step 5. 9. Add 100 µL of Streptavidin-HRP Working Solution to each well. 10. Cover plate with Plate Sealer and incubate at room temperature for 30 minutes. 11. Wash plate four times with 1X Wash Buffer as described in step 5. 12. Add 100 µL of TMB Substrate to each well. 13. Develop the plate in the dark at room temperature for 30 minutes. 14. Stop reaction by adding 100 µL of Stop Solution to each well. 15. Measure absorbance on a plate reader at 450 nm.
Other Resources 1	Kit Booklet
Other Resources 2	MSDS

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHE11900013-1	SKT-111-480	5 X 96 well	$2452		Add to Cart
BHE11900013-2	SKT-111-96	96 well	$576		Add to Cart

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