ELISA Kit for Rat TGF-beta receptor type-2

Mfr.No.

E9935r

Alternative Names

TGF-beta type II receptor ; Transforming growth factor-beta receptor type II ; TGF-beta receptor type II

Categories

Elisa

Function

Transmembrane serine/threonine kinase forming with the TGF-beta type I serine/threonine kinase receptor, TGFBR1, the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and is thus regulating a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. The formation of the receptor complex composed of 2 TGFBR1 and 2 TGFBR2 molecules symetrically bound to the cytokine dimer results in the phosphorylation and the activation of TGFRB1 by the constitutively active TGFBR2. Activated TGFBR1 phosphorylates SMAD2 which dissociates from the receptor and interacts with SMAD4. The SMAD2-SMAD4 complex is subsequently translocated to the nucleus where it modulates the transcription of the TGF-beta-regulated genes. This constitutes the canonical SMAD-dependent TGF-beta signaling cascade. Also involved in non-canonical, SMAD-independent TGF-beta signaling pathways.

Specificity

Natural and recombinant Rat TGF-beta receptor type-2

Subcellular Location

Cell membrane Single-pass type I membrane protein

Detection Range

0.312-20ng/mL

Entryname

TGFR2_RAT

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

Method Type

Sandwich

Platform

PF08917

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

Protein Name

TGF-beta receptor type-2

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

Sensitivity

0.172ng/mL

Sequence

50:

MGRGLLRGLW ; PLHIVLWTRI ; ASTIPPHVPK ; SVNSDLMAGD ; NSGAVKLPQL ;

100:

CKFCDVTLST ; CDNQKSCMSN ; CSVTSICEKP ; QEVCVAVWRK ; NDKNITLETV ;

150:

CHDPKFTYHG ; FTLEDATSPT ; CVMKEKKRAG ; ETFFMCSCNT ; EECNDYIIFN ;

200:

EEYTTSSPDL ; LLVIIQVTGV ; SLLPPLGIAI ; AVIAIFYCYR ; VHRQQKLSPS ;

250:

WESSKPRKLM ; DFSDNCAIIL ; EDDRSDISST ; CANNINHNTE ; LLPIELDTLV ;

300:

GKGRFAEVYK ; AKLKQNTSEQ ; FETVAVKIFP ; YEEYSSWKTE ; KDIFSDINLK ;

350:

HENILQFLTA ; EERKTEMGKQ ; YWLITAFHAK ; GNLQEYLTRH ; VISWEDLRKL ;

400:

GSSLARGIAH ; LHSDHTPCGR ; PKMPIVHRDL ; KSSNILVKND ; LTCCLCDFGL ;

450:

SLRLDPTLSV ; DDLANSGQVG ; TARYMAPEVL ; ESRMNLENME ; SFKQTDVYSM ;

500:

ALVLWEMTSR ; CNAVGEVKDY ; EPPFGSKVRE ; HPCVESMKDN ; VLRDRGRPEI ;

550:

PSFWLNHQGI ; QIVCETLTEC ; WDHDPEARLT ; AQCVAERFSE ; LEHPDRLSGR ;

567:

SCSQEKIPED ; GSLNTTK

Sequence Length

567

String

P38438

Sub Unit

Homodimer. Heterohexamer; TGFB1, TGFB2 and TGFB3 homodimeric ligands assemble a functional receptor composed of two TGFBR1 and TGFBR2 heterodimers to form a ligand-receptor heterohexamer. The respective affinity of TGFRB1 and TGFRB2 for the ligands may modulate the kinetics of assembly of the receptor and may explain the different biological activities of TGFB1, TGFB2 and TGFB3. Interacts with DAXX. Interacts with TCTEX1D4. Interacts with ZFYVE9; ZFYVE9 recruits SMAD2 and SMAD3 to the TGF-beta receptor.

Uniprot	P38438
Gene Name	Tgfbr2
Immunogen Species	Rattus norvegicus (Rat)
Kegg	rno:81810
Unigene	Rn.9954

Reference	1. "Molecular characterization of rat transforming growth factor-beta type II receptor." Tsuchida K., Lewis K.A., Mathews L.S., Vale W.W. Biochem. Biophys. Res. Commun.191:790-795(1993) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] strain: Sprague-Dawley. tissue: Pituitary. 2. "Rat mesangial cell hypertrophy in response to transforming growth factor-beta 1." Choi M.E., Kim E.G., Huang Q., Ballermann B.J. Kidney Int.44:948-958(1993) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]

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BHE13810950	E9935r	96T	$796		Add to Cart

ELISA Kit for Rat TGF-beta receptor type-2

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