Plays an important role in fat metabolism. It preferentially splits the esters of long-chain fatty acids at positions 1 and 3, producing mainly 2-monoacylglycerol and free fatty acids, and shows considerably higher activity against insoluble emulsified substrates than against soluble ones.
Specificity
Natural and recombinant Rat Pancreatic triacylglycerol lipase
Subcellular Location
Secreted
Detection Range
78-5000pg/mL
Entryname
LIPP_RAT
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PNLIP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤8.0%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤9.9%
Protein Name
Pancreatic triacylglycerol lipase
Recovery
Recovery was determined by spiking various levels of PNLIP into serum and plasma .
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