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ELISA Kit for Rat Heparanase

SKU : BHE13805172

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EIAAB SCIENCE INC, WUHAN

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Mfr.No.	E0711r
Alternative Names	Endo-glucoronidase ; Hep
Categories	Elisa
Function	Endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. Participates in extracellular matrix (ECM) degradation and remodeling. Highly selective enzyme cleaving HSPGs at specific intrachain sites. It is essentially inactive at neutral pH but becomes active under acidic conditions such as during tumor invasion and in inflammatory processes. Facilitates cell migration associated with metastasis, wound healing and inflammation. Enhances shedding of syndecans, and increases endothelial invasion and angiogenesis in myelomas. Acts as procoagulant by increasing the generation of activation factor X in the presence of tissue factor and activation factor VII. Increases cell adhesion to the extacellular matrix (ECM), independent of its enzymatic activity. Induces AKT1/PKB phosphorylation via lipid rafts increasing cell mobility and invasion. Heparin increases this AKT1/PKB activation. Regulates osteogenesis. Enhances angiogenesis through up-regulation of SRC-mediated activation of VEGF. Implicated in hair follicle inner root sheath differentiation and hair homeostasis.
Specificity	Natural and recombinant Rat Heparanase
Subcellular Location	Lysosome membrane Peripheral membrane protein Secreted Nucleus Proheparanase is secreted via vesicles of the Golgi. Interacts with cell membrane heparan sulfate proteoglycans (HSPGs). Endocytosed and accumulates in endosomes. Transferred to lysosomes where it is proteolytically cleaved to produce the active enzyme. Under certain stimuli, transferred to the cell surface. Colocalizes with SDC1 in endosomal/lysosomal vesicles. Accumulates in perinuclear lysosomal vesicles. Heparin retains proheparanase in the extracellular medium. Associates with lipid rafts.
Detection Range	0.156-10ng/mL
Entryname	HPSE_RAT
Method Type	Sandwich
Platform	PF03662
Protein Name	Heparanase
Sensitivity	0.078ng/mL
Sequence	50: MLRPLLLLWL ; WGRLGALTQG ; TPAGTAPTKD ; VVDLEFYTKR ; LFQSVSPSFL ; 100: SITIDASLAT ; DPRFLTFLGS ; PRLRALARGL ; SPAYLRFGGT ; KTDFLIFDPN ; 150: KEPTSEERSY ; WQSQDNNDIC ; GSERVSADVL ; RKLQMEWPFQ ; ELLLLREQYQ ; 200: REFKNSTYSR ; SSVDMLYSFA ; KCSRLDLIFG ; LNALLRTPDL ; RWNSSNAQLL ; 250: LNYCSSKGYN ; ISWELGNEPN ; SFWKKAHISI ; DGLQLGEDFV ; ELHKLLQKSA ; 300: FQNAKLYGPD ; IGQPRGKTVK ; LLRSFLKAGG ; EVIDSLTWHH ; YYLNGRVATK ; 350: EDFLSSDVLD ; TFILSVQKIL ; KVTKEMTPGK ; KVWLGETSSA ; YGGGAPLLSD ; 400: TFAAGFMWLD ; KLGLSAQLGI ; EVVMRQVFFG ; AGNYHLVDEN ; FEPLPDYWLS ; 450: LLFKKLVGPK ; VLMSRVKGPD ; RSKLRVYLHC ; TNVYHPRYRE ; GDLTLYVLNL ; 500: HNVTKHLKLP ; PPMFSRPVDK ; YLLKPFGSDG ; LLSKSVQLNG ; QTLKMVDEQT ; 536: LPALTEKPLP ; AGSSLSVPAF ; SYGFFVIRNA ; KIAACI
Sequence Length	536
String	Q71RP1
Sub Unit	Heterodimer; heterodimer formation between the 8 kDa and the 50 kDa subunits is required for enzyme activity (By similarity). Interacts with TF; the interaction, inhibited by heparin, enhances the generation of activated factor X and activates coagulation. Interacts with HRG; the interaction is enhanced at acidic pH, partially inhibits binding of HPSE to cell surface receptors and modulates its enzymatic activity. Interacts with SDC1; the interaction enhances the shedding of SDC1.

Uniprot	Q71RP1
Gene Name	Hpse
Immunogen Species	Rattus norvegicus (Rat)
Kegg	rno:64537
Unigene	Rn.6392

Reference	1. "Cloning of mammalian heparanase, an important enzyme in tumor invasion and metastasis." Hulett M.D., Freeman C., Hamdorf B.J., Baker R.T., Harris M.J., Parish C.R. Nat. Med.5:803-809(1999) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] tissue: Placenta. 2. "Characterization of heparanase from a rat parathyroid cell line." Podyma-Inoue K.A., Yokote H., Sakaguchi K., Ikuta M., Yanagishita M. J. Biol. Chem.277:32459-32465(2002) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];ENZYME REGULATION 3. "Heparanase accelerates wound angiogenesis and wound healing in mouse and rat models." Zcharia E., Zilka R., Yaar A., Yacoby-Zeevi O., Zetser A., Metzger S., Sarid R., Naggi A., Casu B., Ilan N., Vlodavsky I., Abramovitch R. FASEB J.19:211-221(2005) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION

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