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ELISA Kit for Rat E3 ubiquitin-protein ligase UBR4

SKU : BHE13810864

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EIAAB SCIENCE INC, WUHAN

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Mfr.No.	E0192r
Alternative Names	N-recognin-4 ; RING-type E3 ubiquitin transferase UBR4 ; Zinc finger UBR1-type protein 1 ; Rbaf600 ; Zubr1
Categories	Elisa
Function	E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Together with clathrin, forms meshwork structures involved in membrane morphogenesis and cytoskeletal organization. Regulates integrin-mediated signaling. May play a role in activation of FAK in response to cell-matrix interactions.
Specificity	Natural and recombinant Rat E3 ubiquitin-protein ligase UBR4
Subcellular Location	Membrane Multi-pass membrane protein Cytoplasm Cytoplasm cytoskeleton Nucleus Concentrates at the leading edge of membrane structures involved in actin motility.
Detection Range	0.156-10ng/mL
Entryname	UBR4_RAT
Method Type	Sandwich
Platform	PF02207
Protein Name	E3 ubiquitin-protein ligase UBR4
Sensitivity	0.078ng/mL
Sequence	50: MATSGGEEAA ; AAAPAPGAPA ; TGQDTTPGWE ; VAVRPLLSAS ; YSAFEMKELP ; 100: QLVASVIESE ; SEILHHEKQY ; EPFYSSFVAL ; STHYITTVCS ; LIPRNQLQSV ; 150: AAACKVLIEF ; SLLRLENPDE ; ACAVSQKHLI ; LLIKGLCTGC ; SRLDRTEIIT ; 200: FTAMMKSAKL ; PQTVKTLSDV ; EDQKELASPV ; SPELRQKEVQ ; MNFLNQLTSV ; 250: FNPRTVPSPP ; ISPQALVEGE ; NDEQSSTEQA ; SAVKTKNVFI ; AQNVASLQEL ; 300: GGSEKLLRVC ; LNLPYFLRYI ; NRFQDAVVAN ; SFFIMPATVA ; DATAVRNGFH ; 350: SLVIDVTMAL ; DTLSLPVLEP ; LNPSRLQDVT ; VLSLSCLYAG ; VSVATCMAIL ; 400: HVGSAQQVRT ; GSTSSKEEDY ; ESDAATIVQK ; CLEIYDMIGQ ; AISSSRRAGG ; 450: EHFQNFQLLG ; AWCLLNSLFL ; ILHLSPTALA ; DKGKEKDPLA ; ALRVRDILSR ; 500: TKEGVGSPKL ; GPGKGHQGFG ; VLSVILANHA ; IKLLTSLFQD ; LQVEALHKGW ; 550: ETDGPPAVLS ; IMAQSTSTQR ; VQRLIDSVPL ; TNLLLTLLST ; SYRKACVLQR ; 600: QRKGSMSSDA ; SASTDSNTYY ; EDDFSSTEED ; SSQDDDSEPI ; LGQWFEETIS ; 650: PSKEKAAPPP ; PPPPPPLESS ; PRVKSPSKQA ; SGEKGNILAS ; RKDPELFLGL ; 700: ASNILNFITS ; SMLNSRNNFI ; RNYLSVSLSE ; QHMATLASII ; KEVDKDGLKG ; 750: SSDEDFAAAL ; YHFNHSLVTS ; DLQSPNLQNT ; LLQQLGVAPL ; SSGPWPLYIH ; 800: PQGLSVLSRL ; LLIWQHKAGA ; QGDPDVPECL ; KVWDRFLTTM ; KQSALQGVVP ; 850: SETEDLNIEH ; LQLLLLIFHS ; FSEKGRRAIL ; TTLVQSIQEL ; SVNMEVQMRS ; 900: APLILARLLL ; IFDYLLHQYS ; KAPVYLFEQV ; QHNLLSPPFG ; WASGSQDSSS ; 950: SSRRANIPLY ; HGFKEVEENW ; SKHFSSDAAP ; QPRFYCVLST ; EASEEDLNRL ; 1000: DSEACEVLFS ; KLVKYDELYS ; SLTALLAAGS ; QLDTVKRKEN ; KNMTALEACA ; 1050: LQYYFLILWR ; ILGILPPSKT ; YMNQLAMNSP ; EMSECDILHT ; LRWSSRLRIS ; 1100: SYVSWIKDHL ; IRQGMRPEHA ; GSLVELAASK ; CSSVKYDVEI ; VEEYFARQIS ; 1150: SFCSIDCTTV ; LQLHEIPSLQ ; SIYTLDAAVS ; KVQVSLDEHF ; SKMAAETDPH ; 1200: KSSEITKNLL ; PATLQLIDTY ; ASFTRAYLLQ ; NLNEEGSTEK ; PSQEKLHGFA ; 1250: AVLAIGSSRC ; KANTLGPTLV ; QNLPSSVQSV ; CESWNNINTN ; EFPNIGSWRN ; 1300: AFANDTIPSE ; SYISAVQAAH ; LGTLCGQSLP ; LAASLKHALL ; SLVRLTGDLI ; 1350: VWSDEMNPAQ ; VIRALLPLLL ; ESSTESAAEI ; SSNSLERILG ; PAESDEFLAR ; 1400: VYEKLITGCY ; NILANHADPS ; SGLDESVLEE ; CLQYLEKQLE ; SSQARKAMEE ; 1450: FFSDGGELVQ ; IMMATANEDL ; SAKFCNRVLK ; FFTKLFQLTE ; KSPNPSLLHL ; 1500: CGSLAQLACV ; EPVRLQAWLT ; RMTTSPPKDS ; DQLEVIQENR ; QLLQLLTTYI ; 1550: VRENSQVGEG ; VCAVLLGTLT ; PMATDMLANG ; DGTGFPELMV ; VMATLASAGQ ; 1600: GAGHLQLHNA ; AVDWLGRCKK ; YLSQKNVVEK ; LNANVMHGKH ; VMVLECTCHI ; 1650: MSYLADVTNA ; LSQSNGQGPS ; HLSVDGEERA ; IEVDSDWVEE ; LAVEEEDSQA ; 1700: EDSDEDSLCN ; KLCTFTITQK ; EFMNQHWYHC ; HTCKMVDGVG ; VCTVCAKVCH ; 1750: KDHEISYAKY ; GSFFCDCGAK ; EDGSCLALVK ; RTPSSGMSST ; MKESAFQSEP ; 1800: RVSESLVRHA ; STSPADKAKV ; TISDGKVADE ; EKPKKSSLCR ; TVEGCREELQ ; 1850: NQANFSFAPL ; VLDMLNFLMD ; AIQTNFQQAS ; AVGSSSRAQQ ; ALSELHTVDK ; 1900: VVEMTDQLMV ; PTLGSQEGAF ; ENVRMNYSGD ; QGQTIRQLIS ; AHVLRRVAMC ; 1950: VLSSPHGRRQ ; HLAVSHEKGK ; ITVLQLSALL ; KQADSSKRKL ; TLTRLASAPV ; 2000: PFTVLSLTGN ; PCKEDYLAVC ; GLKDCHVLTF ; SSSGSVSDHL ; VLHPQLATGN ; 2050: FIIKAVWLPG ; SQTELAIVTA ; DFVKIYDLSV ; DALSPTFYFL ; LPSSKIRDVT ; 2100: FLFNEEGKNI ; IVIMSSAGYM ; YTQLMEEASS ; AQQGPFYVTN ; VLEINHEDLK ; 2150: DSNSQVAGGG ; VSVYYSHVLQ ; MLFFSYSQGK ; SFAATVSRST ; LEVLQLFPIN ; 2200: IKSSNGGSKT ; SPALCQWSEV ; MNHPGLVCCV ; QQTTGVPLVV ; MVKPDTFLIQ ; 2250: EIKTLPAKAK ; IQDMVAIRHT ; ACNEQQRTTM ; ILLCEDGSLR ; IYMANVENTS ; 2300: YWLQPSLQPS ; SVISIMKPVR ; KRKTATITAR ; TSSQVTFPID ; FFEHNQQLTD ; 2350: VEFGGNDLLQ ; VYNAQQIKHR ; LNSTGMYVAN ; TKPGGFTMEI ; SNNSSTMVMT ; 2400: GMRIQIGTQA ; IERAPSYIEI ; FGRTMQLNLS ; RSRWFDFPFT ; REEALQADRK ; 2450: LNLFIGASVD ; PAGVTMIDAV ; KIYGKTKEQF ; GWPDEPPEDF ; PSASVSSVCP ; 2500: PNLNQSNSTG ; DSDSAAPATT ; SGTVLERLVV ; SSLEALESCF ; AVGPIIEKER ; 2550: NKHAAQELAT ; LLLSLPAPAS ; VQQQSKSLLA ; SLHSSRSAYH ; SHKDQALLSK ; 2600: AVQCLNTSSK ; EGKDLDPEVF ; QRLVITARSI ; AVTRPNNLVH ; FTESKLPQME ; 2650: TEGAEEGKEP ; QKQVEGDGCS ; FITQLVNHFW ; KLHASKPKNA ; FLAPACLPGL ; 2700: THIEATVNAL ; VDIIHGYCTC ; ELDCINTASK ; IYMQMLLCPD ; PAVSFSCKQA ; 2750: LIRVLRPRNK ; RRHVTLPSSP ; RSNTPMGDKD ; DDDDDDADEK ; MQSSGIPDGG ; 2800: HIRQESQEQS ; EVDHGDFEMV ; SESMVLETAE ; NVNNGNPSPL ; EALLAGAEGF ; 2850: PPMLDIPPDA ; DDETMVELAI ; ALSLQQDQQG ; SSSSALGLQS ; LGLSGQAPSS ; 2900: SSLDAGTLSD ; TTASGVCACN ; SSVTLSAPAS ; DDEGSTAATD ; GSTLRTSPAD ; 2950: HGGSVGSESG ; GSAVDSVAGE ; HSVSGRSSAY ; GDATAEGHPA ; GPGSVSSSTG ; 3000: AISTTTGHQE ; GDGSEGEGEG ; EAEGDVHTSN ; RLHMVRLMLL ; ERLLQTLPQL ; 3050: RNVGGVRAIP ; YMQVILMLTT ; DLDGEDEKDK ; GALDNLLAQL ; IAELGMDKKD ; 3100: VSKKNERSAL ; NEVHLVVMRL ; LSVFMSRTKS ; GSKSSICESS ; SLISSATAAA ; 3150: LLSSGAVDYC ; LHVLKSLLEY ; WKGQQSEEEP ; VTTSQLLKPH ; TTSSPPDMSP ; 3200: FFLRQYVKGH ; AADVFEAYTQ ; LLTEMVLRLP ; YQIKKIADTS ; SRIPPPVFDH ; 3250: SWFYFLSEYL ; MIQQTPFVRR ; QVRKLLLFIC ; GSKEKYRQLR ; DLHTLDSHVR ; 3300: GIKKLLEEQG ; IFLRASVVTA ; SSGSALQYDT ; LISLMEHLKA ; CAEIAAQRTI ; 3350: NWQKFCIKDD ; SVLYFLLQVS ; FLVDEGVSPV ; LLQLLSCALC ; GSKVLAALAA ; 3400: STGSSSVASS ; APPAASSGQT ; TTQSKSSTKK ; SKKEKKEKEK ; EGESSGSQED ; 3450: QLCTALVNQL ; NRFADKETLI ; QFLRCFLLES ; NSSSVRWQAH ; CLTLHIYRNS ; 3500: NKAQQELLLD ; LMWSIWPELP ; AYGRKAAQFV ; DLLGYFSLKT ; AQTEKKLKEY ; 3550: SQKAVEILRT ; QNHILTNHPN ; SNIYNTLSGL ; VEFDGYYLES ; DPCLVCNNPE ; 3600: VPFCYIKLSS ; IKVDTRYTTT ; QQVVKLIGSH ; TISKVTVKIG ; DLKRTKMVRT ; 3650: INLYYNNRTV ; QAIVELKNKP ; ARWHKAKKVQ ; LTPGQTEVKI ; DLPLPIVASN ; 3700: LMIEFADFYE ; NYQASTETLQ ; CPRCSASVPA ; NPGVCGNCGE ; NVYQCHKCRS ; 3750: INYDEKDPFL ; CNACGFCKYA ; RFDFMLYAKP ; CCAVDPIENE ; EDRKKAVSNI ; 3800: NTLLDKADRV ; YHQLMGHRPQ ; LENLLCKVNE ; AAPEKPQEDS ; GTAGGISSTS ; 3850: ASVNRYILQL ; AQEYCGDCKN ; SFDELSKIIQ ; KVFASRKELL ; EYDLQQREAA ; 3900: TKSSRTSVQP ; TFTASQYRAL ; SVLGCGHTSS ; TKCYGCASAV ; TEHCITLLRA ; 3950: LATNPALRHI ; LVSQGLIREL ; FDYNLRRGAA ; AIREEVRQLM ; CLLTRDNPEA ; 4000: TQQMNDLIIG ; KVSTALKGHW ; ANPDLASSLQ ; YEMLLLTDSI ; SKEDSCWELR ; 4050: LRCALSLFLM ; AVNIKTPVVV ; ENITLMCLRI ; LQKLIKPPAP ; TSKKNKDVPV ; 4100: EALTTVKPYC ; NEIHAQAQLW ; LKRDPKASYE ; AWKKCLPIRG ; VDGNGKSPSK ; 4150: SELHRLYLTE ; KYVWRWKQFL ; SRRGKRTTPL ; DLKLGHNNWL ; RQVLFTPATQ ; 4200: AARQAACTIV ; EALASVPSRK ; QQVLDLLTSY ; LDELSVAGEC ; AAEYLALYQK ; 4250: LIASCHWKVY ; LAARGVLPYV ; GNLITKEIAR ; LLALEEATLS ; TDLQQGYALK ; 4300: SLTGLLSSFV ; EVESIKRHFK ; SRLVGTVLNG ; YLCLRKLVLQ ; RTKLIDETQD ; 4350: MLLEMLEDMT ; TGTESETKAF ; MAVCIETAKR ; YNLDDYRTPV ; FIFERLCSII ; 4400: YPEENEVTEF ; FVTLEKDPQQ ; EDFLQGRMPG ; NPYSSNEPGI ; GPLMRDIKNK ; 4450: ICQDCDLVAL ; LEDDSGMELL ; VNNKIISLDL ; PVAEVYKKVW ; CTTNEGEPMR ; 4500: IVYRMRGLLG ; DATEEFIESL ; DSTTDEEEDE ; EEVYRMAGVM ; AQCGGLQCML ; 4550: NRLAGVKDFK ; QGRHLLTVLL ; KLFSYCVKVK ; VNRQQLVKLE ; MNTLNVMLGT ; 4600: LNLALVAEQE ; SKDSGGAAVA ; EQVLSIMEII ; LDESNAEPLS ; EDKGNLLLTG ; 4650: DKDQLVMLLD ; QINSTFVRSN ; PSVLQGLLRI ; IPYLSFGEVE ; KMQILVERFK ; 4700: PYCSFDKYDE ; DHSGDDKVFL ; DCFCKIAAGI ; KNNSNGHQLK ; DLILQKGITQ ; 4750: SALDYMKKHI ; PSAKNLDADI ; WKKFLSRPAL ; PFILRLLRGL ; AMQHPATQVL ; 4800: IGTDSITSLH ; KLEQVSSDEG ; IGTLAENLLE ; ALREHPDVNK ; KIDAARRETR ; 4850: AEKKRMAMAM ; RQKALGTLGM ; TTNEKGQVVT ; KTALLKQMEE ; LIEEPGLTCC ; 4900: ICREGYKFQP ; TKVLGIYTFT ; KRVALEEMEN ; KPRKQQGYST ; VSHFNIVHYD ; 4950: CHLAAVRLAR ; GREEWESAAL ; QNANTKCNGL ; LPVWGPHVPE ; SAFATCLARH ; 5000: NTYLQECTGQ ; REPTYQLNIH ; DIKLLFLRFA ; MEQSFSADTG ; GGGRESNIHL ; 5050: IPYIIHTVLY ; VLNTTRATSR ; EEKNLQGFLE ; QPREKWTESA ; FDVDGPHYFT ; 5100: ILALHVLPPE ; QWKATRVEIL ; RRLLVASHAR ; AVAPGGATRL ; TDKAVKDYSA ; 5150: YRSSLLFWAL ; VDLIYNMFKK ; VPTSNTEGGW ; SCSLAEYIRH ; NDMPIYEAAD ; 5194: KALKTFQEEF ; MPVETFSEFL ; DAAGLLSEIT ; DPESFLKDLL ; NSVP
Sequence Length	5194
String	Q2TL32
Sub Unit	Interacts with RB1 and calmodulin.

Uniprot	Q2TL32
Gene Name	Ubr4
Immunogen Species	Rattus norvegicus (Rat)
Kegg	rno:313658
Unigene	Rn.37755

Reference	1. "Expression and characterization of a zinc-finger containing gene (ZUBR1) induced by bright cyclic light." Zhang Q., Anderson R.E. Submitted (2004-12) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] strain: Sprague-Dawley. tissue: Retina. 2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 4841-5194 tissue: Spleen. 3. "Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites." Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A. Proc. Natl. Acad. Sci. U.S.A.103:7159-7164(2006) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1403;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]

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