ELISA Kit for Rat Cell adhesion molecule 3

Mfr.No.

E11089r

Alternative Names

Immunoglobulin superfamily member 4B ; IgSF4B ; Nectin-like protein 1 ; NECL-1 ; Igsf4b ; Necl1

Categories

Elisa

Function

Involved in the cell-cell adhesion. Has both calcium-independent homophilic cell-cell adhesion activity and calcium-independent heterophilic cell-cell adhesion activity with IGSF4, PVRL1 and PVRL3. Interaction with EPB41L1 may regulate structure or function of cell-cell junctions.

Specificity

Natural and recombinant Rat Cell adhesion molecule 3

Subcellular Location

Cell junction Cell membrane Single-pass type I membrane protein Localized at the cell-cell attached sites of the plasma membrane.

Detection Range

0.312-20ng/mL

Entryname

CADM3_RAT

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CADM3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	96-110%	98-107%	95-112%	99-107%
EDTA plasma(n=5)	94-108%	96-105%	93-108%	96-106%
heparin plasma(n=5)	92-110%	94-109%	97-104%	95-105%

Method Type

Sandwich

Platform

PF08205

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.6%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.1%

Protein Name

Cell adhesion molecule 3

Recovery

Recovery was determined by spiking various levels of CADM3 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	103	95-105
Plasma	101	92-107

Sensitivity

0.111ng/mL

Sequence

50:

MGAPSALPLL ; LLLACSWAPG ; GANLSQDDSQ ; PWTSDETVVA ; GGTVVLKCQV ;

100:

KDHEDSSLQW ; SNPAQQTLYF ; GEKRALRDNR ; IQLVSSTPHE ; LSISISNVAL ;

150:

ADEGEYTCSI ; FTMPVRTAKS ; LVTVLGIPQK ; PIITGYKSSL ; REKETATLNC ;

200:

QSSGSKPAAQ ; LAWRKGDQEL ; HGDQTRIQED ; PNGKTFTVSS ; SVSFQVTRDD ;

250:

DGANVVCSVN ; HESLKGADRS ; TSQRIEVLYT ; PTAMIRPEPA ; HPREGQKLLL ;

300:

HCEGRGNPVP ; QQYVWVKEGS ; EPPLKMTQES ; ALIFPFLNKS ; DSGTYGCTAT ;

350:

SNMGSYTAYF ; TLNVNDPSPV ; PSSSSTYHAI ; IGGIVAFIVF ; LLLILLIFLG ;

396:

HYLIRHKGTY ; LTHEAKGSDD ; APDADTAIIN ; AEGGQSGGDD ; KKEYFI

Sequence Length

396

String

Q1WIM3

Sub Unit

Homodimer. Can form trans-heterodimers with PVRL3/nectin-3. Interacts with EPB41L1, DLG3, MPP6 and CASK.

Uniprot	Q1WIM3
Gene Name	Cadm3
Immunogen Species	Rattus norvegicus (Rat)
Kegg	rno:360882
Unigene	Rn.138884

Reference	1. "The nectin-like proteins: candidate cell adhesion molecules to mediate axo-glial interactions." Maurel P., Einheber S., Rubin M.B., Galinska J., Thaker P., Murakami Y., Salzer J.L. Submitted (2005-11) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] strain: Sprague-Dawley.

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BHE13801159	E11089r	96T	$796		Add to Cart

ELISA Kit for Rat Cell adhesion molecule 3

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