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Home / Assay Kits / ELISA Kits

ELISA Kit for Rat Catenin beta-1

SKU : BHE13803482

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EIAAB SCIENCE INC, WUHAN

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Mfr.No.	E1021r
Alternative Names	Beta-catenin ; Catnb
Categories	Elisa
Function	Key dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion. Acts as a negative regulator of centrosome cohesion. Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization.
Specificity	Natural and recombinant Rat Catenin beta-1
Subcellular Location	Cytoplasm Cytoplasm cytoskeleton Nucleus Cell junction adherens junction Cell junction Cell membrane Cytoplasm cytoskeleton centrosome Cytoplasm cytoskeleton spindle pole Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization. The majority of beta-catenin is localized to the cell membrane. In interphase, colocalizes with CROCC between CEP250 puncta at the proximal end of centrioles, and this localization is dependent on CROCC and CEP250. In mitosis, when NEK2 activity increases, it localizes to centrosomes at spindle poles independent of CROCC. Co-localizes with CDK5 in the cell-cell contacts and plasma membrane of undifferentiated and differentiated neuroblastoma cells.
Detection Range	0.156-10ng/mL
Entryname	CTNB1_RAT
Method Type	Sandwich
Platform	PF00514
Protein Name	Catenin beta-1
Sensitivity	0.078ng/mL
Sequence	50: MATQADLMEL ; DMAMEPDRKA ; AVSHWQQQSY ; LDSGIHSGAT ; TTAPSLSGKG ; 100: NPEEEDVDTS ; QVLYEWEQGF ; SQSFTQEQVA ; DIDGQYAMTR ; AQRVRAAMFP ; 150: ETLDEGMQIP ; STQFDAAHPT ; NVQRLAEPSQ ; MLKHAVVNLI ; NYQDDAELAT ; 200: RAIPELTKLL ; NDEDQVVVNK ; AAVMVHQLSK ; KEASRHAIMR ; SPQMVSAIVR ; 250: TMQNTNDVET ; ARCTAGTLHN ; LSHHREGLLA ; IFKSGGIPAL ; VKMLGSPVDS ; 300: VLFYAITTLH ; NLLLHQEGAK ; MAVRLAGGLQ ; KMVALLNKTN ; VKFLAITTDC ; 350: LQILAYGNQE ; SKLIILASGG ; PQALVNIMRT ; YTYEKLLWTT ; SRVLKVLSVC ; 400: SSNKPAIVEA ; GGMQALGPHL ; TDPSQRLVQN ; CLWTLRNLSD ; AATKQEGMEG ; 450: LLGTLVQLLG ; SDDINVVTCA ; AGILSNLTCN ; NYKNKMMVCQ ; VGGIEALVRT ; 500: VLRAGDREDI ; TEPAICALRH ; LTSRHQEAEM ; AQNAVRLHYG ; LPVVVKLLHP ; 550: PSHWPLIKAT ; VGLIRNLALC ; PANHAPLREQ ; GAIPRLVQLL ; VRAHQDTQRR ; 600: TSMGGTQQQF ; VEGVRMEEIV ; EGCTGALHIL ; ARDVHNRIVI ; RGLNTIPLFV ; 650: QLLYSPIENI ; QRVAAGVLCE ; LAQDKEAAEA ; IEAEGATAPL ; TELLHSRNEG ; 700: VATYAAAVLF ; RMSEDKPQDY ; KKRLSVELTS ; SLFRTEPMAW ; NETADLGLDI ; 750: GAQGEALGYR ; QDDPSYRSFH ; SGGYGQDALG ; MDPMMEHEMG ; GHHPGADYPV ; 781: DGLPDLGHAQ ; DLMDGLPPGD ; SNQLAWFDTD ; L
Sequence Length	781
String	Q9WU82
Sub Unit	Two separate complex-associated pools are found in the cytoplasm. The majority is present as component of an E-cadherin/ catenin adhesion complex composed of at least E-cadherin/CDH1 and beta-catenin/CTNNB1, and possibly alpha-catenin/CTNNA1; the complex is located to adherens junctions. The stable association of CTNNA1 is controversial as CTNNA1 was shown not to bind to F-actin when assembled in the complex. Alternatively, the CTNNA1-containing complex may be linked to F-actin by other proteins such as LIMA1. Another cytoplasmic pool is part of a large complex containing AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 Interacts directly with AXIN1; the interaction is regulated by CK2 via BTRC and its subsequent degradation by the proteasome. Interacts directly with AXIN1;the interaction is regulated by CDK2 phosphorylation. Wnt-dependent activation of DVL antagonizes the action of GSK3B. When GSK3B activity is inhibited the complex dissociates, CTNNB1 is dephosphorylated and is no longer targeted for destruction. The stabilized protein translocates to the nucleus, where it binds TCF/LEF-1 family members, TBP, BCL9 and possibly also RUVBL1 and CHD8. Binds CTNNBIP and EP300. CTNNB1 forms a ternary complex with LEF1 and EP300 that is disrupted by CTNNBIP1 binding (By similarity). Interacts with TAX1BP3 (via the PDZ domain); this interaction inhibits the transcriptional activity of CTNNB1 (By similarity). Interacts with AJAP1, BAIAP1, CARM1, CTNNA3, CXADR and PCDH11Y. Binds SLC9A3R1. Interacts with GLIS2. Interacts with XIRP1 (By similarity). Interacts with PTPRU (via the cytoplasmic juxtamembrane domain) and with SLC30A9. Interacts with EMD (By similarity). Interacts with SCRIB (By similarity). Interacts with TNIK and TCF7L2 (By similarity). Interacts with SESTD1 and TRPC4 (By similarity). Interacts directly with AXIN1; the interaction is regulated by CDK2 phosphorylation of AXIN1. Interacts with CAV1. Interacts with TRPV4. The TRPV4 and CTNNB1 complex can interact with CDH1. Interacts with VCL. Interacts with PTPRJ (By similarity). Interacts with PKT7 (By similarity). Interacts with FAT1 (via the cytoplasmic domain). Interacts with NANOS1 (By similarity). Interacts with CDK2, NEK2 and CDK5 (By similarity). Found in a complex composed of MACF1, APC, AXIN1, CTNNB1 and GSK3B.

Uniprot	Q9WU82
Gene Name	Ctnnb1
Immunogen Species	Rattus norvegicus (Rat)
Kegg	rno:84353
Unigene	Rn.112601

Reference	1. "Study on the formation of specialized inter-Sertoli cell junctions in vitro." Chung S.S.W., Lee W.M., Cheng C.Y. J. Cell. Physiol.181:258-272(1999) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] strain: Sprague-Dawley. tissue: Testis. 2. "Sequencing of the rat beta-catenin gene (Ctnnb1) and mutational analysis of liver tumors induced by 2-amino-3-methylimidazo[4,5-f]quinoline." Li Q., Dixon B.M., Al-Fageeh M., Blum C.A., Dashwood R.H. Gene283:255-262(2002) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] strain: Fischer 344. 3. "The role of microtubule actin cross-linking factor 1 (MACF1) in the Wnt signaling pathway." Chen H.J., Lin C.M., Lin C.S., Perez-Olle R., Leung C.L., Liem R.K. Genes Dev.20:1933-1945(2006) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION;IDENTIFICATION IN A COMPLEX WITH MACF1; APC; AXIN1 AND GSK3B 4. "The Fat1 cadherin integrates vascular smooth muscle cell growth and migration signals." Hou R., Liu L., Anees S., Hiroyasu S., Sibinga N.E. J. Cell Biol.173:417-429(2006) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH FAT1 5. "A novel beta-catenin-binding protein inhibits beta-catenin-dependent Tcf activation and axis formation." Sakamoto I., Kishida S., Fukui A., Kishida M., Yamamoto H., Hino S., Michiue T., Takada S., Asashima M., Kikuchi A. J. Biol. Chem.275:32871-32878(2000) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH CHD8 6. "Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites." Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A. Proc. Natl. Acad. Sci. U.S.A.103:7159-7164(2006) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-675;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]

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