ELISA Kit for Rat Cadherin-1

Mfr.No.

E0017r

Alternative Names

Epithelial cadherin ; E-cadherin ; Uvomorulin ; CD324

Categories

Elisa

Function

E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.

Specificity

Natural and recombinant Rat Cadherin-1

Subcellular Location

Cell junction Cell membrane Single-pass type I membrane protein Endosome Golgi apparatus trans-Golgi network Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane.

Detection Range

0.156-10ng/mL

Entryname

CADH1_RAT

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	93-105%	94-103%	95-105%	92-108%
EDTA plasma(n=5)	89-98%	93-101%	90-99%	90-104%
heparin plasma(n=5)	93-101%	92-102%	99-109%	94-107%

Method Type

Sandwich

Platform

PF00028

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤ 6.8%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤ 11.2%

Protein Name

Cadherin-1

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	102	96-105
Plasma	104	94-106

Sensitivity

0.084ng/mL

Sequence

50:

MGARCRSFSA ; LLLLLQVSSW ; LCQQPESESD ; SCRPGFSSEV ; YTFLVPERHL ;

100:

ERGHILGRVK ; FEGCTGRPRT ; AFFSEDSRFK ; VSTDGVITVK ; RHLKLHKLET ;

150:

SFLVHAWDSS ; YRKLSTKVTL ; KSLGHHHHRH ; HHRDPVSESN ; PELLTFPSFH ;

200:

QGLRRQKRDW ; VIPPINCPEN ; QKGEFPQRLV ; QIKSNRDKET ; TVFYSITGPG ;

250:

ADKPPVGVFI ; IERETGWLKV ; TQPLDREAID ; KYLLYSHAVS ; SNGEAVEDPM ;

300:

EIVVTVTDQN ; DNRPEFIQEV ; FEGSVAEGAL ; PGTSVMQVSA ; TDADDDINTY ;

350:

NAAIAYTILS ; QDPELPHKNM ; FTVNRDTGVI ; SVVTSGLDRE ; SYPTYTLVVQ ;

400:

AADLQGEGLS ; TTAKAVITVK ; DINDNAPIFN ; PSTYQGQVLE ; NEVGARIATL ;

450:

KVTDDDAPNT ; PAWNAVYTVV ; NDPDHQFTVI ; TDPKTNEGIL ; KTAKGLDFEA ;

500:

KQQYILHVTV ; ENEEPFEGSL ; VPSTATVTVD ; VVDVNEAPIF ; VPAEKRVEVP ;

550:

EDFGVGLEIA ; SYTAREPDTF ; MEQKITYRIW ; RDTANWLEIN ; PETGVISTRA ;

600:

EMDREDSEHV ; KNSTYTALII ; ATDDGSPIAT ; GTGTLLLVLS ; DVNDNAPIPE ;

650:

PRNMQFCQRN ; PKPHVITILD ; PDLPPNTSPF ; TAELTHGASV ; NWTIEYNDAE ;

700:

QESLILQPRK ; DLEIGEYKIN ; LKLSDNQNKD ; QVTTLEVHVC ; DCEGTVNNCM ;

750:

KAISLEAGLQ ; VPAILGILGG ; ILALLILILL ; LLLFLRRRTV ; VKEPLLPPDD ;

800:

DTRDNVYYYD ; EEGGGEEDQD ; FDLSQLHRGL ; DARPEVIRND ; VAPTLMSMPQ ;

850:

YRPRPANPDE ; IGNFIDENLK ; AADSDPTAPP ; YDSLLVFDYE ; GSGSEAASLS ;

886:

SLNSSESDQD ; QDYDYLNEWG ; NRFKKLADMY ; GGGEED

Sequence Length

886

String

Q9R0T4

Sub Unit

Homodimer; disulfide-linked. Component of an E-cadherin/ catenin adhesion complex composed of at least E-cadherin/CDH1, beta-catenin/CTNNB1 or gamma-catenin/JUP, and potentially alpha-catenin/CTNNA1; the complex is located to adherens junctions. The stable association of CTNNA1 is controversial as CTNNA1 was shown not to bind to F-actin when assembled in the complex. Alternatively, the CTNNA1-containing complex may be linked to F-actin by other proteins such as LIMA1. Interaction with PSEN1, cleaves CDH1 resulting in the disassociation of cadherin-based adherens junctions (CAJs). Interacts with AJAP1, CTNND1 and DLGAP5 (By similarity). Interacts with TBC1D2. Interacts with LIMA1. Interacts with CAV1. Interacts with the TRPV4 and CTNNB1 complex.

Uniprot	Q9R0T4
Gene Name	Cdh1
Immunogen Species	Rattus norvegicus (Rat)
Kegg	rno:83502
Unigene	Rn.1303

Reference	1. Asai K., Tada T., Yamamoto M., Obayashi M., Mizuno M., Toda A., Eimoto T., Kato T. Submitted (1998-09) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] strain: Sprague-Dawley. tissue: Jejunum. 2. "Multiple cadherin superfamily members with unique expression profiles are produced in rat testis." Johnson K.J., Patel S.R., Boekelheide K. Endocrinology141:675-683(2000) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 769-872 strain: Fischer 344. tissue: Testis. 3. Gibbons K.L. [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 779-846 tissue: Mammary tumor. 4. "Rab8B GTPase and junction dynamics in the testis." Lau A.S., Mruk D.D. Endocrinology144:1549-1563(2003) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH RAB8B

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE13801624	E0017r	96T	$796		Add to Cart

ELISA Kit for Rat Cadherin-1

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of ELISA Kit for Rat Cadherin-1

Rate it now..!!