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Home / Assay Kits / ELISA Kits

ELISA Kit for Rat Axin-1

SKU : BHE13801356

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EIAAB SCIENCE INC, WUHAN

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Mfr.No.	E9657r
Alternative Names	Axis inhibition protein 1 ; rAxin ; Axin
Categories	Elisa
Function	Component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling. Controls dorsoventral patterning via two opposing effects; down-regulates beta-catenin to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway. Also facilitates the phosphorylation of APC by GSK3B. Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation. Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7.
Specificity	Natural and recombinant Rat Axin-1
Subcellular Location	Cytoplasm Nucleus Cell membrane On UV irradiation, translocates to the nucleus and colocalizes with DAAX (By similarity). MACF1 is required for its translocation to cell membrane.
Detection Range	0.156-10ng/ml
Entryname	AXIN1_RAT
Method Type	Sandwich
Platform	PF08833
Protein Name	Axin-1
Sensitivity	0.078ng/mL
Sequence	50: MNVQEQGFPL ; DLGASFTEDA ; PRPPVPGEEG ; ELVSTDSRPV ; NHSFCSGKGT ; 100: SIKSETSTAT ; PRRSDLDLGY ; EPEGSASPTP ; PYLRWAESLH ; SLLDDQDGIS ; 150: LFRTFLKQEG ; CADLLDFWFA ; CSGFRKLEPC ; DSNEEKRLKL ; ARAIYRKYIL ; 200: DSNGIVSRQT ; KPATKSFIKD ; CVMKQQIDPA ; MFDQAQTEIQ ; STMEENTYPS ; 250: FLKSDIYLEY ; TRTGSESPKV ; CSDQSSGSGT ; GKGMSGYLPT ; LNEDEEWKCD ; 300: QDADEDDGRD ; SVPPSRLTQK ; LLLETAAPRA ; PSSRRYNEGR ; ELRYGSWREP ; 350: VNPYYVNSGY ; ALAPATSAND ; SEQQSLSSDA ; DTLSLTDSSV ; DGIPPYRIRK ; 400: QHRREMQESV ; QVNGRVPLPH ; IPRTYRMPKE ; IRVEPQKFAE ; ELIHRLEAVQ ; 450: RTREAEEKLE ; ERLKRVRMEE ; EGEDGEMPSG ; PMASHKLPSV ; PAWHHFPPRY ; 500: VDMGCSGLRD ; AHEENPESIL ; DEHVQRVMRT ; PGCQSPGPGH ; RSPDSGHVAK ; 550: TAVLGGTASG ; HGKHAPKLGL ; KLDSAGLHHH ; RHVHHHVHHN ; SARPKEQMEA ; 600: EAARRVQSSF ; SWGPETHGHA ; KPRSYSESTG ; TNPSAGDLAF ; GGKASAPSKR ; 650: NTKKAESGKN ; ASAEVPSTTE ; DAEKNQKIMQ ; WIIEGEKEIS ; RHRKAGHGSS ; 700: GMRKQQAHES ; SRPLSIERPG ; AVHPWVSAQL ; RNSVQPSHLF ; IQDPTMPPNP ; 750: APNPLTQLEE ; ARRRLEEEEK ; RANKLPSKQR ; TKSQRKAGGG ; SAPPCDSIVV ; 800: AYYFCGEPIP ; YRTLVRGRAV ; TLGQFKELLT ; KKGSYRYYFK ; KVSDEFDCGV ; 827: VFEEVREDEA ; ILPVFEEKII ; GKVEKVD
Sequence Length	827
String	O70239
Sub Unit	Homodimer (By similarity). Component of the AXIN1-HIPK2-TP53 complex (By similarity). Interacts directly in the complex with TP53 and HIPK2. Interacts with DAXX; the interaction stimulates the interaction of DAXX with TP53, stimulates 'Ser-46' phosphorylation of TP53 and induces cell death on UV irradiation. Also binds ANKRD6, PIAS1, PIAS2, PIAS4, MAP3K1, MAP3K4, SUMO1, SMAD6, SMAD7 and RNF111. Interacts with DIXDC1; the interaction prevents interaction with MAP3K1. Interacts with MDFI; the interaction decreases AXIN1-mediated JUN N-terminal kinase (JNK) activation. Interacts with MDFIC; the interaction inhibits beta-cateninin-mediated signaling and AXIN1-mediated JUN N-terminal kinase (JNK) activation. Interacts with LRP5 (via its phosphorylated PPPSP motifs); the interaction is stimulated by WNT1 and GSK3B and activates beta-catenin signaling. Interacts (via the C-terminal) with PPP1CA; the interaction dephosphorylates AXIN1 and regulates interaction with GSK3B. Interacts with PPP2CA; the interaction dephosphorylates AXIN1 (By similarity). Component of the beta-catenin destruction complex, containing at least, CTNNB1, an axin and GSK3B, that regulates CTNNB1 protein levels through phosphorylation and ubiquitination. Interacts with CTNNB1 (via the armadillo repeats 2-7). Interacts with GSK3B; the interaction hyperphosphorylates CTNNB1 leading to its ubiquitination and destruction. Interacts with MACF1. Found in a complex composed of MACF1, APC, AXIN1, CTNNB1 and GSK3B.

Uniprot	O70239
Gene Name	Axin1
Immunogen Species	Rattus norvegicus (Rat)
Kegg	rno:79257
Unigene	Rn.31781

Reference	1. "Axin, a negative regulator of the Wnt signaling pathway, forms a complex with GSK-3beta and beta-catenin and promotes GSK-3beta-dependent phosphorylation of beta-catenin." Ikeda S., Kishida S., Yamamoto H., Murai H., Koyama S., Kikuchi A. EMBO J.17:1371-1384(1998) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];PHOSPHORYLATION;INTERACTION WITH GSK3B AND CNNB1 IN THE BETA-CATENIN DESTRUCTION COMPLEX;TISSUE SPECIFICITY tissue: Brain. 2. "The role of microtubule actin cross-linking factor 1 (MACF1) in the Wnt signaling pathway." Chen H.J., Lin C.M., Lin C.S., Perez-Olle R., Leung C.L., Liem R.K. Genes Dev.20:1933-1945(2006) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION;INTERACTION WITH MACF1;IDENTIFICATION IN A COMPLEX WITH MACF1; APC; GSK3B AND CTNNB1

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SKU	Supplier No.	Size	Your price	Quantity
BHE13801356	E9657r	96T	$796		Add to Cart

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