3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I ; 3-beta-HSD I
Categories
Elisa
Function
3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.
Specificity
Natural and recombinant Rat 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
Subcellular Location
Endoplasmic reticulum membrane Single-pass membrane protein Mitochondrion membrane Single-pass membrane protein
Detection Range
78-5000pg/mL
Entryname
3BHS1_RAT
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤3.9%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.7%
Protein Name
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
Recovery
Recovery was determined by spiking various levels of ACE into serum and plasma .