Secretogranin V ; Secretogranin-5 ; Secretory granule endocrine protein I ; SGNE1
Categories
Elisa
Function
Acts as a molecular chaperone for PCSK2/PC2, preventing its premature activation in the regulated secretory pathway. Binds to inactive PCSK2 in the endoplasmic reticulum and facilitates its transport from there to later compartments of the secretory pathway where it is proteolytically matured and activated. Also required for cleavage of PCSK2 but does not appear to be involved in its folding. Plays a role in regulating pituitary hormone secretion. The C-terminal peptide inhibits PCSK2 in vitro.
Specificity
Natural and recombinant Pig Neuroendocrine protein 7B2
Subcellular Location
Secreted Neuroendocrine and endocrine secretory granules.
Entryname
7B2_PIG
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤ 4.7%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤ 7.1%
Protein Name
Neuroendocrine protein 7B2
Recovery
Recovery was determined by spiking various levels of ACE into serum and plasma .