ELISA Kit for Pig Kynurenine 3-monooxygenase

Mfr.No.

E13545p

Alternative Names

Fpk ; Kynurenine 3-hydroxylase

Categories

Elisa

Function

Catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn). Required for synthesis of quinolinic acid, a neurotoxic NMDA receptor antagonist and potential endogenous inhibitor of NMDA receptor signaling in axonal targeting, synaptogenesis and apoptosis during brain development. Quinolinic acid may also affect NMDA receptor signaling in pancreatic beta cells, osteoblasts, myocardial cells, and the gastrointestinal tract.

Specificity

Natural and recombinant Pig Kynurenine 3-monooxygenase

Subcellular Location

Mitochondrion outer membrane Multi-pass membrane protein

Entryname

KMO_PIG

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of KMO and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	95-107%	93-110%	96-105%	98-104%
EDTA plasma(n=5)	90-108%	92-106%	91-107%	92-105%
heparin plasma(n=5)	93-104%	94-106%	93-104%	94-106%

Platform

PF01494

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤7.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.4%

Protein Name

Kynurenine 3-monooxygenase

Recovery

Recovery was determined by spiking various levels of KMO into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	101	92-106
Plasma	99	95-105

Sequence

50:

MDSSDIQRTS ; IAVIGGGLVG ; SLNACFLAKR ; NFQVDVYESR ; EDIRMAEFAR ;

100:

GRSINLALSY ; RGRQALKAIG ; LEDQIVSQGI ; PMRARMIHSL ; SGKKSAIPYG ;

150:

TKSQYILSIS ; RENLNKDLLT ; AVEKYPNAKV ; HFGHQLLKCR ; PETGVITLLG ;

200:

PDKVPKDIAC ; DLILGCDGAY ; STVRTHLVKK ; PRFDYSQQYI ; PHGYMELTIP ;

250:

PQNGDFAMEP ; NYLHIWPRDT ; FMMIALPNMN ; KSFTCTLFMP ; FEEFEKLLTS ;

300:

RDVLDFFQKY ; FPDSLHLIGK ; EALAQDFFRL ; PAQPMISVKC ; SSFHFNSHCV ;

350:

LMGDAAHALV ; PFFGQGMNAG ; FEDCLVFDEL ; MDKFNNDFSM ; CLPEFSKFRI ;

400:

PDDHAISDLS ; MYNYIEMRSH ; VNSRWFIFQK ; NIERCLHTLM ; PSTFIPLYTM ;

450:

VTFSRIRYHE ; AMLRWQWQKK ; VINTALFFFG ; TLVALSTTYL ; LTGPTFRSSL ;

471:

GCLRRSWNSV ; TYFQNIGRIS ; L

Sequence Length

471

Uniprot	Q9MZS9
Gene Name	KMO
Immunogen Species	Sus Scrofa (Pig)
Kegg	ssc:397148
Unigene	Ssc.283

Reference	1. "Purification of L-kynurenine 3-monooxygenase from mitochondrial outer membrane of pig liver." Uemura T., Hirai K. Adv. Exp. Med. Biol.467:619-623(1999) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] tissue: Liver.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13806851	E13545p	96T	$900		Add to Cart

ELISA Kit for Pig Kynurenine 3-monooxygenase

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