ELISA Kit for Pig Interleukin-27 subunit alpha

Mfr.No.

E0385p

Alternative Names

p28 ; IL27A

Categories

Elisa

Function

Cytokine with pro- and anti-inflammatory properties, that can regulate T helper cell development, suppress T-cell proliferation, stimulate cytotoxic T cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR which appears to be required but not sufficient for IL-27-mediated signal transduction. IL-27 potentiate the early phase of TH1 response and suppress TH2 and TH17 differentiation. It induces the differentiation of TH1 cells via two distinct pathways, p38 MAPK/TBX21- and ICAM1/ITGAL/ERK-dependent pathways. It also induces STAT1, STAT3, STAT4 and STAT5 phosphorylation and activates TBX21/T-Bet via STAT1 with resulting IL12RB2 up-regulation, an event crucial to TH1 cell commitment. It suppresses the expression of GATA3, the inhibitor TH1 cells development. In CD8 T-cells, it activates STATs as well as GZMB. IL-27 reveals to be a potent inhibitor of TH17 cell development and of IL-17 production. Indeed IL-27 subunit p28 alone is also able to inhibit the production of IL17 by CD4 and CD8 T-cells. While IL-27 suppressed the development of proinflammatory Th17 cells via STAT1, it inhibits the development of anti-inflammatory inducible regulatory T-cells, iTreg, independently of STAT1. IL-27 has also an effect on cytokine production, it suppresses proinflammatory cytokine production such as IL2, IL4, IL5 and IL6 and activates suppressors of cytokine signaling such as SOCS1 and SOCS3. Apart from suppression of cytokine production, IL-27 also antagonizes the effects of some cytokines such as IL6 through direct effects on T cells. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines such as IP-10/CXCL10 and MIG/CXCL9. In vein endothelial cells, it induces IRF1/interferon regulatory factor 1 and increase the expression of MHC class II transactivator/CIITA with resulting up-regulation of major histocompatibility complex class II. IL-27 also demonstrates antiviral activity with inhibitory properties on HIV-1 replivation.

Specificity

Natural and recombinant Pig Interleukin-27 subunit alpha

Subcellular Location

Secreted Does not seem to be secreted without coexpression of EBI3.

Detection Range

31.2-2000pg/mL

Entryname

IL27A_PIG

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL27A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	100-106%	98-106%	95-105%	103-109%
EDTA plasma(n=5)	95-100%	102-107%	97-104%	102-108%
heparin plasma(n=5)	98-105%	106-110%	100-108%	96-108%

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.2%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.8%

Protein Name

Interleukin-27 subunit alpha

Recovery

Recovery was determined by spiking various levels of IL27A into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	104	100-108
Plasma	103	98-105

Sensitivity

14.4pg/mL

Sequence

50:

MGQMADDLGW ; RLSLLLLSLL ; LARAGVWGFP ; RPPGRPPLSL ; QELQREFKVS ;

100:

LHLARKLLSE ; VRVQARHFAE ; SHLPGVNLDL ; LPLGEQLPNV ; SLNFQAWRGL ;

150:

SDPERLCFLS ; MTLRPFHTLL ; GGLGSQGFWT ; SSERMQLWAI ; RLDLRDLQQH ;

200:

LRFQVLAAGF ; NLPGQEEEEN ; EAGRELLPGA ; PGGPSKPAAQ ; VSWPRLLYTY ;

239:

QLLHSLELVL ; SRAMRDFLLL ; SRAGNPAPAL ; GFPTPSSPP

Sequence Length

239

Sub Unit

Heterodimer with EBI3. IL-27 is an heterodimer composed IL-27 subunit alpha and EBI3. EBI3 is also a component of the IL-12 heterodimer.

Uniprot	Q5S1V9
Gene Name	IL27
Immunogen Species	Sus Scrofa (Pig)
Kegg	ssc:493187
Unigene	Ssc.36782

Reference	1. Peng H. Submitted (2004-10) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]

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SKU	Supplier No.	Size	Your price	Quantity
BHE13805944	E0385p	96T	$900		Add to Cart

ELISA Kit for Pig Interleukin-27 subunit alpha

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