ELISA Kit for Pig Hepcidin

Mfr.No.

E1979p

Categories

Elisa

Function

Seems to act as a signaling molecule involved in the maintenance of iron homeostasis. Seems to be required in conjunction with HFE to regulate both intestinal iron absorption and iron storage in macrophages. May also have antimicrobial activity.

Specificity

Natural and recombinant Pig Hepcidin

Subcellular Location

Secreted

Detection Range

0.78-50ng/mL

Entryname

HEPC_PIG

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HEPC and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	91-105%	92-103%	92-100%	94-102%
EDTA plasma(n=5)	94-100%	95-102%	91-107%	90-105%
heparin plasma(n=5)	93-104%	95-100%	93-103%	92-104%

Method Type

Sandwich

Platform

PF06446

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.4%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.9%

Protein Name

Hepcidin

Recovery

Recovery was determined by spiking various levels of HEPC into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	97	90-100
Plasma	98	95-102

Sensitivity

0.24ng/mL

Sequence

50:

MALSVQIRAA ; CLLLLLLVSL ; TAGSVLPSQT ; RQLTDLRTQD ; TAGATAGLTP ;

82:

VAQRLRRDTH ; FPICIFCCGC ; CRKAICGMCC ; KT

Sequence Length

String

Q8MJ80

Uniprot	Q8MJ80
Gene Name	HAMP
Immunogen Species	Sus Scrofa (Pig)
Kegg	ssc:397207
Unigene	Ssc.376

Reference	1. "Cloning and characterization of porcine liver-expressed antimicrobial peptides, Hepcidin (pHAMP) and LEAP2." Sang Y., Ross C.R., Blecha F. Submitted (2002-05) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE13805428	E1979p	96T	$900		Add to Cart

ELISA Kit for Pig Hepcidin

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of ELISA Kit for Pig Hepcidin

Rate it now..!!