ELISA Kit for Pig Fibroblast growth factor 1

Mfr.No.

E0032p

Alternative Names

Acidic fibroblast growth factor ; aFGF ; Endothelial cell growth factor ; ECGF ; Heparin-binding growth factor 1 ; HBGF-1

Categories

Elisa

Function

Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro.

Specificity

Natural and recombinant Pig Fibroblast growth factor 1

Subcellular Location

Secreted Cytoplasm Cytoplasm cell cortex Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1.

Detection Range

78-5000pg/mL

Entryname

FGF1_PIG

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FGF1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-100%	97-104%	95-98%	91-96%
EDTA plasma(n=5)	89-93%	102-105%	94-97%	93-98%
heparin plasma(n=5)	91-95%	96-99%	106-108%	87-93%

Method Type

Sandwich

Platform

PF00167

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.7%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤6.8%

Protein Name

Fibroblast growth factor 1

Recovery

Recovery was determined by spiking various levels of FGF1 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	95	93-97
Plasma	97	96-102

Sensitivity

19pg/mL

Sequence

50:

MAEGEITTFT ; ALTEKFNLPP ; GNYKKPKLLY ; CSNGGHFLRI ; LPDGTVDGTR ;

100:

DRSDQHIQLQ ; LSAESVGEVY ; IKSTETGQYL ; AMDTSGLLYG ; SQTPSEECLF ;

150:

LERLEENHYN ; TYTSKKHAEK ; NWFVGLKKNG ; SCKRGPRTHY ; GQKAILFLPL ;

152:

Sequence Length

152

String

P20002

Sub Unit

Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Found in a complex with FGFBP1, FGF1 and FGF2. Interacts with FGFBP1. Part of a Cu(2+)-dependent multiprotein aggregate containing FGF1, S100A13 and SYT1. Interacts with SYT1. Interacts with S100A13.

Uniprot	P20002
Gene Name	FGF1
Immunogen Species	Sus Scrofa (Pig)
Unigene	Ssc.16246

Reference	1. "Amplification and sequencing of mRNA encoding acidic fibroblast growth factor (aFGF) from porcine heart." Schmidt M., Sharma H.S., Schott R.J., Schaper W. Biochem. Biophys. Res. Commun.180:853-859(1991) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] tissue: Heart. 2. "Isolation of heparin-binding growth factors from bovine, porcine and canine hearts." Quinkler W., Maasberg M., Bernotat-Danielowski S., Luethe N., Sharma H.S., Schaper W. Eur. J. Biochem.181:67-73(1989) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 22-41

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SKU	Supplier No.	Size	Your price	Quantity
BHE13804810	E0032p	96T	$900		Add to Cart

ELISA Kit for Pig Fibroblast growth factor 1

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