ELISA Kit for Pig Connective tissue growth factor

Mfr.No.

E0010p

Alternative Names

CCN family member 2 ; CCN2

Categories

Elisa

Function

Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes (By similarity). Mediates heparin-and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells (By similarity). Enhances fibroblast growth factor-induced DNA synthesis.

Specificity

Natural and recombinant Pig Connective tissue growth factor

Subcellular Location

Secreted extracellular space extracellular matrix Secreted

Detection Range

0.312-20ng/mL

Entryname

CTGF_PIG

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CTGF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	98-105%	100-106%	92-98%	92-103%
EDTA plasma(n=5)	89-98%	93-100%	95-102%	96-103%
heparin plasma(n=5)	99-104%	96-105%	101-108%	92-99%

Method Type

Sandwich

Platform

PF00007

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.0%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.8%

Protein Name

Connective tissue growth factor

Recovery

Recovery was determined by spiking various levels of CTGF into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	95-103
Plasma	98	96-101

Sensitivity

0.15ng/mL

Sequence

50:

MSATGLSPVR ; CAFVLLLALC ; SRPASGQDCS ; GQCQCAAGKR ; RACPAGVSLV ;

100:

LDGCGCCRLC ; AKQLGELCTE ; RDPCDPHKGL ; FCDFGSPANR ; KIGVCTAKDG ;

150:

APCVFGGTVY ; RSGESFQSSC ; KYQCTCLDGA ; VGCVPLCSMD ; VRLPSPDCPF ;

200:

PRRVKLPGKC ; CEEWVCDEPK ; DHTVVGPALA ; AYRLEDTFGP ; DPTMMRANCL ;

250:

VQTTEWSACS ; KTCGMGISTR ; VTNDNAFCRL ; EKQSRLCMVR ; PCEADLEENI ;

300:

KKGKKCIRTP ; KISKPVKFEL ; SGCTSVKTYR ; AKFCGVCTDG ; RCCTPHRTTT ;

349:

LPVEFKCPDG ; EVMKKSMMFI ; KTCACHYNCP ; GDNDIFESLY ; YRKMYGDMA

Sequence Length

349

String

O19113

Sub Unit

Monomer.

Uniprot	O19113
Gene Name	CTGF
Immunogen Species	Sus Scrofa (Pig)
Unigene	Ssc.70001

Reference	1. "Purification and characterization of novel heparin-binding growth factors in uterine secretory fluids. Identification as heparin-regulated Mr 10,000 forms of connective tissue growth factor." Brigstock D.R., Steffen C.L., Kim G.Y., Vegunta R.K., Diehl J.R., Harding P.A. J. Biol. Chem.272:20275-20282(1997) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] tissue: Uterus.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13803615	E0010p	96T	$900		Add to Cart

ELISA Kit for Pig Connective tissue growth factor

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