ELISA Kit for Mouse Tumor necrosis factor receptor superfamily member 11B

Mfr.No.

E0108m

Alternative Names

Osteoclastogenesis inhibitory factor ; Osteoprotegerin ; Ocif ; Opg

Categories

Elisa

Function

Acts as decoy receptor for RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local RANKL/OPG ratio. May also play a role in preventing arterial calcification. May act as decoy receptor for TRAIL and protect against apoptosis. TRAIL binding blocks the inhibition of osteoclastogenesis.

Specificity

Natural and recombinant Mouse Tumor necrosis factor receptor superfamily member 11B

Subcellular Location

Secreted

Detection Range

7.8-500pg/mL

Entryname

TR11B_MOUSE

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TR11B and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	93-100%	93-99%	95-102%	93-98%
EDTA plasma(n=5)	90-96%	95-102%	101-105%	94-103%
heparin plasma(n=5)	94-99%	96-104%	95-100%	92-104%

Method Type

Sandwich

Platform

PF00531

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.1%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.9%

Protein Name

Tumor necrosis factor receptor superfamily member 11B

Recovery

Recovery was determined by spiking various levels of TR11B into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	97	92-98
Plasma	98	95-102

Sensitivity

4.13pg/mL

Sequence

50:

MNKWLCCALL ; VLLDIIEWTT ; QETLPPKYLH ; YDPETGHQLL ; CDKCAPGTYL ;

100:

KQHCTVRRKT ; LCVPCPDHSY ; TDSWHTSDEC ; VYCSPVCKEL ; QSVKQECNRT ;

150:

HNRVCECEEG ; RYLEIEFCLK ; HRSCPPGSGV ; VQAGTPERNT ; VCKKCPDGFF ;

200:

SGETSSKAPC ; IKHTNCSTFG ; LLLIQKGNAT ; HDNVCSGNRE ; ATQKCGIDVT ;

250:

LCEEAFFRFA ; VPTKIIPNWL ; SVLVDSLPGT ; KVNAESVERI ; KRRHSSQEQT ;

300:

FQLLKLWKHQ ; NRDQEMVKKI ; IQDIDLCESS ; VQRHLGHSNL ; TTEQLLALME ;

350:

SLPGKKISPE ; EIERTRKTCK ; SSEQLLKLLS ; LWRIKNGDQD ; TLKGLMYALK ;

400:

HLKTSHFPKT ; VTHSLRKTMR ; FLHSFTMYRL ; YQKLFLEMIG ; NQVQSVKISC ;

401:

Sequence Length

401

String

O08712

Sub Unit

Homodimer.

Uniprot	O08712
Gene Name	Tnfrsf11b
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:18383
Unigene	Mm.15383

Reference	1. "Osteoprotegerin: a novel secreted protein involved in the regulation of bone density." Simonet W.S., Lacey D.L., Dunstan C.R., Kelley M., Chang M.-S., Luethy R., Nguyen H.Q., Wooden S., Bennett L., Boone T., Shimamoto G., Derose M., Elliott R., Colombero A., Tan H.-L., Trail G., Sullivan J., Davy E., Bucay N., Renshaw-Gegg L., Hughes T.M., Hill D., Pattison W., Campbell P., Sander S., Van G., Tarpley J., Derby P., Lee R., Suggs S., Boyle W.J.more... Cell89:309-319(1997) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] strain: BALB/c. tissue: Kidney. 2. "Structure of the mouse osteoclastogenesis inhibitory factor (OCIF) gene and its expression in embryogenesis." Mizuno A., Murakami A., Nakagawa N., Yasuda H., Tsuda E., Morinaga T., Higashio K. Gene215:339-343(1998) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA];VARIANTS PRO-138; ARG-161; ASP-165; ALA-288 AND ARG-296 strain: 129/Ola. strain: NIH Swiss. tissue: Fibroblast. 3. "Osteoprotegerin reverses osteoporosis by inhibiting endosteal osteoclasts and prevents vascular calcification by blocking a process resembling osteoclastogenesis." Min H., Morony S., Sarosi I., Dunstan C.R., Capparelli C., Scully S., Van G., Kaufman S., Kostenuik P.J., Lacey D.L., Boyle W.J., Simonet W.S. J. Exp. Med.192:463-474(2000) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION 4. "RANKL employs distinct binding modes to engage RANK and the osteoprotegerin decoy receptor." Nelson C.A., Warren J.T., Wang M.W., Teitelbaum S.L., Fremont D.H. Structure20:1971-1982(2012) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 22-197 IN COMPLEX WITH TNFSF11/RANKL;DISULFIDE BONDS

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ELISA Kit for Mouse Tumor necrosis factor receptor superfamily member 11B

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