ELISA Kit for Mouse Talin-1

Mfr.No.

E2104m

Alternative Names

Tln

Categories

Elisa

Function

Probably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts.

Specificity

Natural and recombinant Mouse Talin-1

Subcellular Location

Cell projection ruffle membrane Peripheral membrane protein Cytoplasmic side Cytoplasm cytoskeleton Colocalizes with LAYN at the membrane ruffles.

Detection Range

0.312-20ng/ml

Entryname

TLN1_MOUSE

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TLN1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	90-102%	93-100%	89-102%	91-100%
Saliva(n=3)	94-99%	91-103%	91-105%	90-102%
heparin plasma(n=5)	91-102%	92-104%	93-101%	93-100%

Method Type

Sandwich

Platform

PF00373

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.5%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤11.3%

Protein Name

Talin-1

Recovery

Recovery was determined by spiking various levels of TLN1 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	96	92-104
Plasma	97	93-102

Sensitivity

0.171ng/mL

Sequence

50:

MVALSLKISI ; GNVVKTMQFE ; PSTMVYDACR ; MIRERIPEAL ; AGPPNDFGLF ;

100:

LSDDDPKKGI ; WLEAGKALDY ; YMLRNGDTME ; YRKKQRPLKI ; RMLDGTVKTI ;

150:

MVDDSKTVTD ; MLMTICARIG ; ITNHDEYSLV ; RELMEEKKDE ; GTGTLRKDKT ;

200:

LLRDEKKMEK ; LKQKLHTDDE ; LNWLDHGRTL ; REQGVEEHET ; LLLRRKFFYS ;

250:

DQNVDSRDPV ; QLNLLYVQAR ; DDILNGSHPV ; SFDKACEFAG ; FQCQIQFGPH ;

300:

NEQKHKAGFL ; DLKDFLPKEY ; VKQKGERKIF ; QAHKNCGQMS ; EIEAKVRYVK ;

350:

LARSLKTYGV ; SFFLVKEKMK ; GKNKLVPRLL ; GITKECVMRV ; DEKTKEVIQE ;

400:

WSLTNIKRWA ; ASPKSFTLDF ; GDYQDGYYSV ; QTTEGEQIAQ ; LIAGYIDIIL ;

450:

KKKKSKDHFG ; LEGDEESTML ; EDSVSPKKST ; VLQQQYNRVG ; KVEHGSVALP ;

500:

AIMRSGASGP ; ENFQVGSMPP ; AQQQITSGQM ; HRGHMPPLTS ; AQQALTGTIN ;

550:

SSMQAVQAAQ ; ATLDDFETLP ; PLGQDAASKA ; WRKNKMDESK ; HEIHSQVDAI ;

600:

TAGTASVVNL ; TAGDPAETDY ; TAVGCAVTTI ; SSNLTEMSRG ; VKLLAALLED ;

650:

EGGNGRPLLQ ; AAKGLAGAVS ; ELLRSAQPAS ; AEPRQNLLQA ; AGNVGQASGE ;

700:

LLQQIGESDT ; DPHFQDVLMQ ; LAKAVASAAA ; ALVLKAKSVA ; QRTEDSGLQT ;

750:

QVIAAATQCA ; LSTSQLVACT ; KVVAPTISSP ; VCQEQLVEAG ; RLVAKAVEGC ;

800:

VSASQAATED ; GQLLRGVGAA ; ATAVTQALNE ; LLQHVKAHAT ; GAGPAGRYDQ ;

850:

ATDTILTVTE ; NIFSSMGDAG ; EMVRQARILA ; QATSDLVNAI ; KADAEGESDL ;

900:

ENSRKLLSAA ; KILADATAKM ; VEAAKGAAAH ; PDSEEQQQRL ; REAAEGLRMA ;

950:

TNAAAQNAIK ; KKLVQRLEHA ; AKQAAASATQ ; TIAAAQHAAS ; APKASAGPQP ;

1000:

LLVQSCKAVA ; EQIPLLVQGV ; RGSQAQPDSP ; SAQLALIAAS ; QSFLQPGGKM ;

1050:

VAAAKASVPT ; IQDQASAMQL ; SQCAKNLGTA ; LAELRTAAQK ; AQEACGPLEM ;

1100:

DSALSVVQNL ; EKDLQEIKAA ; ARDGKLKPLP ; GETMEKCTQD ; LGNSTKAVSS ;

1150:

AIAKLLGEIA ; QGNENYAGIA ; ARDVAGGLRS ; LAQAARGVAA ; LTSDPAVQAI ;

1200:

VLDTASDVLD ; KASSLIEEAK ; KASGHPGDPE ; SQQRLAQVAK ; AVTQALNRCV ;

1250:

SCLPGQRDVD ; NALRAVGDAS ; KRLLSDSLPP ; STGTFQEAQS ; RLNEAAAGLN ;

1300:

QAATELVQAS ; RGTPQDLARA ; SGRFGQDFST ; FLEAGVEMAG ; QAPSQEDRAQ ;

1350:

VVSNLKGISM ; SSSKLLLAAK ; ALSTDPASPN ; LKSQLAAAAR ; AVTDSINQLI ;

1400:

TMCTQQAPGQ ; KECDNALRQL ; ETVRELLENP ; VQPINDMSYF ; GCLDSVMENS ;

1450:

KVLGEAMTGI ; SQNAKNGNLP ; EFGDAIATAS ; KALCGFTEAA ; AQAAYLVGVS ;

1500:

DPNSQAGQQG ; LVEPTQFARA ; NQAIQMACQS ; LGEPGCTQAQ ; VLSAATIVAK ;

1550:

HTSALCNSCR ; LASARTANPT ; AKRQFVQSAK ; EVANSTANLV ; KTIKALDGDF ;

1600:

TEENRAQCRA ; ATAPLLEAVD ; NLSAFASNPE ; FSSVPAQISP ; EGRAAMEPIV ;

1650:

ISAKTMLESA ; GGLIQTARAL ; AVNPRDPPRW ; SVLAGHSRTV ; SDSIKKLITS ;

1700:

MRDKAPGQLE ; CETAIAALNS ; CLRDLDQASL ; AAVSQQLAPR ; EGISQEALHT ;

1750:

QMLTAVQEIS ; HLIEPLASAA ; RAEASQLGHK ; VSQMAQYFEP ; LTLAAVGAAS ;

1800:

KTLSHPQQMA ; LLDQTKTLAE ; SALQLLYTAK ; EAGGNPKQAA ; HTQEALEEAV ;

1850:

QMMTEAVEDL ; TTTLNEAASA ; AGVVGGMVDS ; ITQAINQLDE ; GPMGDPEGSF ;

1900:

VDYQTTMVRT ; AKAIAVTVQE ; MVTKSNTSPE ; ELGPLANQLT ; SDYGRLASQA ;

1950:

KPAAVAAENE ; EIGAHIKHRV ; QELGHGCSAL ; VTKAGALQCS ; PSDVYTKKEL ;

2000:

IECARRVSEK ; VSHVLAALQA ; GNRGTQACIT ; AASAVSGIIA ; DLDTTIMFAT ;

2050:

AGTLNREGAE ; TFADHREGIL ; KTAKVLVEDT ; KVLVQNAAGS ; QEKLAQAAQS ;

2100:

SVATITRLAD ; VVKLGAASLG ; AEDPETQVVL ; INAVKDVAKA ; LGDLISATKA ;

2150:

AAGKVGDDPA ; VWQLKNSAKV ; MVTNVTSLLK ; TVKAVEDEAT ; KGTRALEATT ;

2200:

EHIRQELAVF ; CSPEPPAKTS ; TPEDFIRMTK ; GITMATAKAV ; AAGNSCRQED ;

2250:

VIATANLSRR ; AIADMLRACK ; EAAFHPEVAP ; DVRLRALHYG ; RECANGYLEL ;

2300:

LDHVLLTLQK ; PNPDLKQQLT ; GHSKRVAGSV ; TELIQAAEAM ; KGTEWVDPED ;

2350:

PTVIAENELL ; GAAAAIEAAA ; KKLEQLKPRA ; KPKEADESLN ; FEEQILEAAK ;

2400:

SIAAATSALV ; KAASAAQREL ; VAQGKVGAIP ; ANALDDGQWS ; QGLISAARMV ;

2450:

AAATNNLCEA ; ANAAVQGHAS ; QEKLISSAKQ ; VAASTAQLLV ; ACKVKADQDS ;

2500:

EAMKRLQAAG ; NAVKRASDNL ; VKAAQKAAAF ; EDQENETVVV ; KEKMVGGIAQ ;

2541:

IIAAQEEMLR ; KERELEEARK ; KLAQIRQQQY ; KFLPSELRDE ; H

Sequence Length

2541

String

P26039

Sub Unit

Binds with high affinity to vinculin and with low affinity to integrins. Interacts with NRAP and LAYN. Interacts with SYNM.

Uniprot	P26039
Gene Name	Tln1
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:21894
Unigene	Mm.208601

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE13811438	E2104m	96T	$776		Add to Cart

ELISA Kit for Mouse Talin-1

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of ELISA Kit for Mouse Talin-1

Rate it now..!!