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ELISA Kit for Mouse Runt-related transcription factor 1

SKU : BHE13809924

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EIAAB SCIENCE INC, WUHAN

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Mfr.No.	E10487m
Alternative Names	Acute myeloid leukemia 1 protein ; Core-binding factor subunit alpha-2 ; CBF-alpha-2 ; Oncogene AML-1 ; Polyomavirus enhancer-binding protein 2 alpha B subunit ; PEA2-alpha B ; SL3-3 enhancer factor 1 alpha B subunit ; SL3/AKV core-binding factor alpha B subunit ; Aml1 ; Cbfa2 ; Pebp2ab
Categories	Elisa
Function	CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. Essential for the development of normal hematopoiesis. Isoform 4 shows higher binding activities for target genes and binds TCR-beta-E2 and RAG-1 target site with threefold higher affinity than other isoforms. It is less effective in the context of neutrophil terminal differentiation. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the BLK promoter. Inhibits KAT6B-dependent transcriptional activation.
Specificity	Natural and recombinant Mouse Runt-related transcription factor 1
Subcellular Location	Nucleus
Entryname	RUNX1_MOUSE
Platform	PF00853
Protein Name	Runt-related transcription factor 1
Sequence	50: MRIPVDASTS ; RRFTPPSTAL ; SPGKMSEALP ; LGAPDGGPAL ; ASKLRSGDRS ; 100: MVEVLADHPG ; ELVRTDSPNF ; LCSVLPTHWR ; CNKTLPIAFK ; VVALGDVPDG ; 150: TLVTVMAGND ; ENYSAELRNA ; TAAMKNQVAR ; FNDLRFVGRS ; GRGKSFTLTI ; 200: TVFTNPPQVA ; TYHRAIKITV ; DGPREPRRHR ; QKLDDQTKPG ; SLSFSERLSE ; 250: LEQLRRTAMR ; VSPHHPAPTP ; NPRASLNHST ; AFNPQPQSQM ; QDARQIQPSP ; 300: PWSYDQSYQY ; LGSITSSSVH ; PATPISPGRA ; SGMTSLSAEL ; SSRLSTAPDL ; 350: TAFGDPRQFP ; TLPSISDPRM ; HYPGAFTYSP ; PVTSGIGIGM ; SAMSSASRYH ; 400: TYLPPPYPGS ; SQAQAGPFQT ; GSPSYHLYYG ; ASAGSYQFSM ; VGGERSPPRI ; 450: LPPCTNASTG ; AALLNPSLPS ; QSDVVETEGS ; HSNSPTNMPP ; ARLEEAVWRP ; 451: Y
Sequence Length	451
String	Q03347
Sub Unit	Heterodimer with CBFB. RUNX1 binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with TLE1 and THOC4. Interacts with HIPK2, ELF1, ELF2 and SPI1. Interacts via its Runt domain with the ELF4 N-terminal region. Interaction with ELF2 isoform 2 (NERF-1a) may act to repress RUNX1-mediated transactivation. Interacts with KAT6A and KAT6B. Interacts with SUV39H1, leading to abrogation of transactivating and DNA-binding properties of RUNX1 (By similarity). Interacts with YAP1. Interaction with CDK6 prevents myeloid differentiation, reducing its transcription transactivation activity.

Uniprot	Q03347
Gene Name	Runx1
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:12394
Unigene	Mm.4081

Reference	1. "Isolation of PEBP2 alpha B cDNA representing the mouse homolog of human acute myeloid leukemia gene, AML1." Bae S.-C., Yamaguchi-Iwai Y., Ogawa E., Maruyama M., Inuzuka M., Kagoshima H., Shigesada K., Satake M., Ito Y. Oncogene8:809-814(1993) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1) tissue: Fibroblast. 2. "PEBP2 alpha B/mouse AML1 consists of multiple isoforms that possess differential transactivation potentials." Bae S.-C., Ogawa E., Maruyama M., Oka H., Satake M., Shigesada K., Jenkins N.A., Gilbert D.J., Copeland N.G., Ito Y. Mol. Cell. Biol.14:3242-3252(1994) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2) tissue: Fibroblast. 3. Calabi F. Submitted (1996-04) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3) strain: BALB/c. tissue: Thymus. 4. "Identification and expression of a novel 3'-exon of mouse Runx1/Pebp2alphaB/Cbfa2/AML1 gene." Tsuji K., Noda M. Biochem. Biophys. Res. Commun.274:171-176(2000) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PARTIAL NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5) 5. "Identification of an alternatively spliced form of the mouse AML1/RUNX1 gene transcript AML1c and its expression in early hematopoietic development." Fujita Y., Nishimura M., Taniwaki M., Abe T., Okuda T. Biochem. Biophys. Res. Commun.281:1248-1255(2001) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PARTIAL NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4) strain: 129/Ola. tissue: Embryo. 6. "Expression and function of a stem cell promoter for the murine CBFalpha2 gene: distinct roles and regulation in natural killer and T cell development." Telfer J.C., Rothenberg E.V. Dev. Biol.229:363-382(2001) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PARTIAL NUCLEOTIDE SEQUENCE [GENOMIC DNA];ALTERNATIVE SPLICING (ISOFORM 4) strain: C57BL/6. tissue: Thymus. 7. "AML1, the target of multiple chromosomal translocations in human leukemia, is essential for normal fetal liver hematopoiesis." Okuda T., van Deursen J., Hiebert S.W., Grosveld G., Downing J.R. Cell84:321-330(1996) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN LIVER HEMATOPOIESIS 8. "The histone- and PRMT5-associated protein COPR5 is required for myogenic differentiation." Paul C., Sardet C., Fabbrizio E. Cell Death Differ.19:900-908(2012) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION IN A COMPLEX WITH PRMT5; RUNX1 AND CBFB 9. "Disruption of the Cbfa2 gene causes necrosis and hemorrhaging in the central nervous system and blocks definitive hematopoiesis." Wang Q., Stacy T., Binder M., Marin-Padilla M., Sharpe A.H., Speck N.A. Proc. Natl. Acad. Sci. U.S.A.93:3444-3449(1996) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN NECROSIS AND HEMORRHAGING 10. "Roles of HIPK1 and HIPK2 in AML1- and p300-dependent transcription, hematopoiesis and blood vessel formation." Aikawa Y., Nguyen L.A., Isono K., Takakura N., Tagata Y., Schmitz M.L., Koseki H., Kitabayashi I. EMBO J.25:3955-3965(2006) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH HIPK2;PHOSPHORYLATION AT SER-249 AND SER-276 BY HIPK2;MUTAGENESIS OF SER-249 AND SER-276 11. "Crystallization and preliminary X-ray analyses of quaternary, ternary and binary protein-DNA complexes with involvement of AML1/Runx-1/CBFalpha Runt domain, CBFbeta and the C/EBPbeta bZip region." Tahirov T.H., Inoue-Bungo T., Sasaki M., Shiina M., Kimura K., Sato K., Kumasaka T., Yamamoto M., Kamiya N., Ogata K. Acta Crystallogr. D57:850-853(2001) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.65 ANGSTROMS) OF 62-177 IN COMPLEX WITH CEBPB; CBFB AND DNA 12. "Structural analyses of DNA recognition by the AML1/Runx-1 Runt domain and its allosteric control by CBFbeta." Tahirov T.H., Inoue-Bungo T., Morii H., Fujikawa A., Sasaki M., Kimura K., Shiina M., Sato K., Kumasaka T., Yamamoto M., Ishii S., Ogata K. Cell104:755-767(2001) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.65 ANGSTROMS) OF 60-182 IN COMPLEX WITH CEBPB; CBFB AND DNA;MUTAGENESIS OF ARG-80; ASN-109; TYR-113; ARG-142; LYS-144; THR-149; VAL-170; ASP-171; ARG-174 AND ARG-177 13. "The RUNX1 Runt domain at 1.25A resolution: a structural switch and specifically bound chloride ions modulate DNA binding." Baeckstroem S., Wolf-Watz M., Grundstroem C., Haerd T., Grundstroem T., Sauer U.H. J. Mol. Biol.322:259-272(2002) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.25 ANGSTROMS) OF 60-173

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