ELISA Kit for Mouse Pyruvate kinase PKM

Mfr.No.

E0588m

Antigen

PKM

Alternative Names

Pyruvate kinase muscle isozyme ; Pk3 ; Pkm2 ; Pykm

Categories

Elisa

Function

Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation.

Specificity

Natural and recombinant Mouse Pyruvate kinase PKM

Subcellular Location

Cytoplasm Nucleus

Detection Range

0.78-50U/mL

Entryname

KPYM_MOUSE

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

Method Type

Sandwich

Platform

PF00224

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

Protein Name

Pyruvate kinase PKM

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

Sensitivity

0.38U/mL

Sequence

50:

MPKPHSEAGT ; AFIQTQQLHA ; AMADTFLEHM ; CRLDIDSAPI ; TARNTGIICT ;

100:

IGPASRSVEM ; LKEMIKSGMN ; VARLNFSHGT ; HEYHAETIKN ; VREATESFAS ;

150:

DPILYRPVAV ; ALDTKGPEIR ; TGLIKGSGTA ; EVELKKGATL ; KITLDNAYME ;

200:

KCDENILWLD ; YKNICKVVEV ; GSKIYVDDGL ; ISLQVKEKGA ; DFLVTEVENG ;

250:

GSLGSKKGVN ; LPGAAVDLPA ; VSEKDIQDLK ; FGVEQDVDMV ; FASFIRKAAD ;

300:

VHEVRKVLGE ; KGKNIKIISK ; IENHEGVRRF ; DEILEASDGI ; MVARGDLGIE ;

350:

IPAEKVFLAQ ; KMMIGRCNRA ; GKPVICATQM ; LESMIKKPRP ; TRAEGSDVAN ;

400:

AVLDGADCIM ; LSGETAKGDY ; PLEAVRMQHL ; IAREAEAAIY ; HLQLFEELRR ;

450:

LAPITSDPTE ; AAAVGAVEAS ; FKCCSGAIIV ; LTKSGRSAHQ ; VARYRPRAPI ;

500:

IAVTRNPQTA ; RQAHLYRGIF ; PVLCKDAVLN ; AWAEDVDLRV ; NLAMDVGKAR ;

531:

GFFKKGDVVI ; VLTGWRPGSG ; FTNTMRVVPV ; P

Sequence Length

531

String

P52480

Sub Unit

Monomer and homotetramer. Exists as a monomer in the absence of fructose 1,6 bi-phosphate (FBP), and reversibly associates to form a homotetramer in the presence of FBP. The monomeric form binds T3. Tetramer formation induces pyruvate kinase activity. Interacts with HERC1, POU5F1 and PML.

Uniprot	P52480
Gene Name	Pkm
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:18746
Unigene	Mm.326167

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ELISA Kit for Mouse Pyruvate kinase PKM

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