PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
Specificity
Natural and recombinant Mouse Protein kinase C epsilon type
Entryname
KPCE_MOUSE
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of KPCE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤5.4%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤8.3%
Protein Name
Protein kinase C epsilon type
Recovery
Recovery was determined by spiking various levels of KPCE into serum and plasma .
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH ACTIN;ACTIN-BINDING MOTIF
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-710;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION;PHOSPHORYLATION AT SER-729;MUTAGENESIS OF SER-729
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT SER-234; SER-316 AND SER-368
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH YWHAB;PHOSPHORYLATION AT SER-346; SER-350 AND SER-368;MUTAGENESIS OF SER-346 AND SER-368
Reviews of ELISA Kit for Mouse Protein kinase C epsilon type