ELISA Kit for Mouse Placenta growth factor

Mfr.No.

E0114m

Alternative Names

Plgf

Categories

Elisa

Function

Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1.

Specificity

Natural and recombinant Mouse Placenta growth factor

Subcellular Location

Secreted

Detection Range

7.8-500pg/mL

Entryname

PLGF_MOUSE

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PLGF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	96-110%	98-107%	99-105%	97-109%
EDTA plasma(n=5)	95-107%	92-108%	97-105%	93-107%
heparin plasma(n=5)	93-110%	94-107%	96-104%	91-110%

Method Type

Sandwich

Platform

PF00341

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤7.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤11.2%

Protein Name

Placenta growth factor

Recovery

Recovery was determined by spiking various levels of PLGF into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	103	96-107
Plasma	101	93-106

Sensitivity

4.14pg/mL

Sequence

50:

MLVMKLFTCF ; LQVLAGLAVH ; SQGALSAGNN ; STEVEVVPFN ; EVWGRSYCRP ;

100:

MEKLVYILDE ; YPDEVSHIFS ; PSCVLLSRCS ; GCCGDEGLHC ; VPIKTANITM ;

150:

QILKIPPNRD ; PHFYVEMTFS ; QDVLCECRPI ; LETTKAERRK ; TKGKRKRSRN ;

158:

SQTEEPHP

Sequence Length

158

String

P49764

Sub Unit

Antiparallel homodimer; disulfide-linked. Also found as heterodimer with VEGFA/VEGF.

Uniprot	P49764
Gene Name	Pgf
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:18654
Unigene	Mm.4809

Reference	1. "The placenta growth factor gene of the mouse." Dipalma T., Tucci M., Russo G., Maglione D., Lago C.T., Romano A., Saccone S., della Valle G., de Gregorio L., Dragani T.A., Viglietto G., Persico M.G. Mamm. Genome7:6-12(1996) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] tissue: Heart. 2. "Placenta growth factor and vascular endothelial growth factor are co-expressed during early embryonic development." Achen M.G., Gad J.M., Stacker S.A., Wilks A.F. Growth Factors15:69-80(1997) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] strain: NIH Swiss. 3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] strain: 129. tissue: Mammary gland.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13808761	E0114m	96T	$776		Add to Cart

ELISA Kit for Mouse Placenta growth factor

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