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ELISA Kit for Mouse Perforin-1

SKU : BHE13812219

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EIAAB SCIENCE INC, WUHAN

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Mfr.No.	E1317m
Alternative Names	Cytolysin ; Lymphocyte pore-forming protein ; Pfp
Categories	Elisa
Function	Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes.
Specificity	Natural and recombinant Mouse Perforin-1
Subcellular Location	Cytoplasmic granule lumen Secreted Cell membrane Multi-pass membrane protein Endosome lumen Stored in cytoplasmic granules of cytolytic T-lymphocytes and secreted into the cleft between T-lymphocyte and target cell. May be taken up via endocytosis involving clathrin-coated vesicles and accumulate in a first time in large early endosomes (By similarity). Inserts into the cell membrane of target cells and forms pores. Membrane insertion and pore formation requires a major conformation change.
Detection Range	15.6-1000pg/mL
Entryname	PERF_MOUSE
Method Type	Sandwich
Platform	PF00168
Protein Name	Perforin-1
Sensitivity	7.8pg/mL
Sequence	50: MATCLFLLGL ; FLLLPRPVPA ; PCYTATRSEC ; KQKHKFVPGV ; WMAGEGMDVT ; 100: TLRRSGSFPV ; NTQRFLRPDR ; TCTLCKNSLM ; RDATQRLPVA ; ITHWRPHSSH ; 150: CQRNVAAAKV ; HSTEGVAREA ; AANINNDWRV ; GLDVNPRPEA ; NMRASVAGSH ; 200: SKVANFAAEK ; TYQDQYNFNS ; DTVECRMYSF ; RLVQKPPLHL ; DFKKALRALP ; 250: RNFNSSTEHA ; YHRLISSYGT ; HFITAVDLGG ; RISVLTALRT ; CQLTLNGLTA ; 300: DEVGDCLNVE ; AQVSIGAQAS ; VSSEYKACEE ; KKKQHKMATS ; FHQTYRERHV ; 350: EVLGGPLDST ; HDLLFGNQAT ; PEQFSTWTAS ; LPSNPGLVDY ; SLEPLHTLLE ; 400: EQNPKREALR ; QAISHYIMSR ; ARWQNCSRPC ; RSGQHKSSHD ; SCQCECQDSK ; 450: VTNQDCCPRQ ; RGLAHLVVSN ; FRAEHLWGDY ; TTATDAYLKV ; FFGGQEFRTG ; 500: VVWNNNNPRW ; TDKMDFENVL ; LSTGGPLRVQ ; VWDADYGWDD ; DLLGSCDRSP ; 550: HSGFHEVTCE ; LNHGRVKFSY ; HAKCLPHLTG ; GTCLEYAPQG ; LLGDPPGNRS ; 554: GAVW
Sequence Length	554
String	P10820
Sub Unit	Monomer, as sobluble protein. Homooligomer. Oligomerization is required for pore formation.

Uniprot	P10820
Gene Name	Prf1
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:18646
Unigene	Mm.240313

Reference	1. "The structure of the mouse lymphocyte pore-forming protein perforin." Kwon B.S., Wakulchik M., Liu C.C., Persechini P.M., Trapani J.A., Haq A.K., Kim Y., Young J.D.-E. Biochem. Biophys. Res. Commun.158:1-10(1989) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] 2. "Genomic organization of the mouse pore-forming protein (perforin) gene and localization to chromosome 10. Similarities to and differences from C9." Trapani J.A., Kwon B.S., Kozak C.A., Chintamaneni C., Young J.D., Dupont B. J. Exp. Med.171:545-557(1990) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] 3. "Homology of perforin to the ninth component of complement (C9)." Shinkai Y., Takio K., Okumura K. Nature334:525-527(1988) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];PROTEIN SEQUENCE OF N-TERMINUS;FUNCTION;SUBCELLULAR LOCATION;TISSUE SPECIFICITY 4. "Cloning, analysis, and expression of murine perforin 1 cDNA, a component of cytolytic T-cell granules with homology to complement component C9." Lowrey D.M., Aebischer Y., Olsen K., Lichtenheld M., Rupp F., Hengartner H., Podack E.R. Proc. Natl. Acad. Sci. U.S.A.86:247-251(1989) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];TISSUE SPECIFICITY 5. Mueller C., Lowin B., Tschopp J. Submitted (1992-11) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] strain: C57BL/6. 6. "Structure and function of the murine perforin promoter and upstream region. Reciprocal gene activation or silencing in perforin positive and negative cells." Lichtenheld M.G., Podack E.R. J. Immunol.149:2619-2626(1992) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-117 strain: BALB/c. 7. "The primary structure of the lymphocyte pore-forming protein perforin: partial amino acid sequencing and determination of isoelectric point." Persechini P.M., Young J.D.-E. Biochem. Biophys. Res. Commun.156:740-745(1988) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 21-52; 76-96; 160-187; 255-278; 312-328; 400-420 AND 439-464 8. "Subcellular localization of perforin and serine esterase in lymphokine-activated killer cells and cytotoxic T cells by immunogold labeling." Ojcius D.M., Zheng L.M., Sphicas E.C., Zychlinsky A., Young J.D.-E. J. Immunol.146:4427-4432(1991) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION;TISSUE SPECIFICITY 9. "Cytotoxicity mediated by T cells and natural killer cells is greatly impaired in perforin-deficient mice." Kagi D., Ledermann B., Burki K., Seiler P., Odermatt B., Olsen K.J., Podack E.R., Zinkernagel R.M., Hengartner H. Nature369:31-37(1994) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;SUBCELLULAR LOCATION;DISRUPTION PHENOTYPE 10. "Immune function in mice lacking the perforin gene." Walsh C.M., Matloubian M., Liu C.C., Ueda R., Kurahara C.G., Christensen J.L., Huang M.T., Young J.D., Ahmed R., Clark W.R. Proc. Natl. Acad. Sci. U.S.A.91:10854-10858(1994) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;DISRUPTION PHENOTYPE 11. "The molecular basis for perforin oligomerization and transmembrane pore assembly." Baran K., Dunstone M., Chia J., Ciccone A., Browne K.A., Clarke C.J.P., Lukoyanova N., Saibil H., Whisstock J.C., Voskoboinik I., Trapani J.A. Immunity30:684-695(2009) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBUNIT;FUNCTION;SUBCELLULAR LOCATION;MUTAGENESIS OF ASP-191; ARG-213 AND GLU-343 12. "The structural basis for membrane binding and pore formation by lymphocyte perforin." Law R.H., Lukoyanova N., Voskoboinik I., Caradoc-Davies T.T., Baran K., Dunstone M.A., D'Angelo M.E., Orlova E.V., Coulibaly F., Verschoor S., Browne K.A., Ciccone A., Kuiper M.J., Bird P.I., Trapani J.A., Saibil H.R., Whisstock J.C. Nature468:447-451(2010) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.75 ANGSTROMS) OF 21-554 OF MUTANT GLU-213 IN COMPLEX WITH CALCIUM;ELECTRON MICROSCOPY OF PORE COMPLEX;FUNCTION;SUBCELLULAR LOCATION;SUBUNIT;GLYCOSYLATION AT ASN-204;DISULFIDE BONDS;CALCIUM-BINDING

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