ELISA Kit for Mouse Paired immunoglobulin-like type 2 receptor beta

Mfr.No.

E13291m

Alternative Names

Activating receptor PILR-beta ; Cell surface receptor FDFACT ; Fdfact ; Pilrb1

Categories

Elisa

Function

Paired receptors consist of highly related activating and inhibitory receptors and are widely involved in the regulation of the immune system. PILRB is thought to act as a cellular signaling activating receptor that associates with ITAM-bearing adapter molecules on the cell surface. Seems to associate with DAP12 and is a receptor for CD99. May be involved in target cell recognition by natural killer cells and in activation of dendritic cells.

Specificity

Natural and recombinant Mouse Paired immunoglobulin-like type 2 receptor beta

Subcellular Location

Membrane Single-pass type I membrane protein

Detection Range

0.156-10ng/mL

Entryname

PILRB_MOUSE

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PILRB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	93-99%	91-102%	95-100%	92-101%
EDTA plasma(n=5)	96-100%	95-103%	95-102%	93-104%
heparin plasma(n=5)	94-104%	94-102%	92-105%	96-101%

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤8.5%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤11.6%

Protein Name

Paired immunoglobulin-like type 2 receptor beta

Recovery

Recovery was determined by spiking various levels of PILRB into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	97	91-99
Plasma	98	93-102

Sensitivity

0.09ng/mL

Sequence

50:

MALLISLPGG ; TPAMAQVLLL ; LSSGCLHAGN ; SERYNRKNGF ; GVNQPERCSG ;

100:

VQGGSIDIPF ; SFYFPWKLAK ; DPQMSIAWKW ; KDFHGEVIYN ; SSLPFIHEHF ;

150:

KGRLILNWTQ ; GQTSGVLRIL ; NLKESDQAQY ; FSRVNLQSTE ; GMKLWQSIPG ;

200:

TQLNVTQALN ; TTMRSPFIVT ; SEFTTAGLEH ; TSDQRNPSLM ; NLGAMVTMLL ;

224:

AKVLVIVLVY ; GWMIFLRWKQ ; RPAH

Sequence Length

224

String

Q2YFS2

Sub Unit

Interacts with CD99. Probably associates with DAP12.

Uniprot	Q2YFS2
Gene Name	Pilrb
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:170741
Unigene	Mm.347593

Reference	1. "Activation of natural killer cells and dendritic cells upon recognition of a novel CD99-like ligand by paired immunoglobulin-like type 2 receptor." Shiratori I., Ogasawara K., Saito T., Lanier L.L., Arase H. J. Exp. Med.199:525-533(2004) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];FUNCTION;INTERACTION WITH CD99;TISSUE SPECIFICITY 2. Bates E.E. Submitted (2000-04) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] 3. "Comparative genomic analysis of the paired immunoglobin-like receptor locus at 7q22: duplications, conversions, inversions and the birth of new genes." Wilson M.D., McKinnel L., Danby A., Schnupf P., Hunt P., Martindale D., Koop B.F. Submitted (2004-11) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] strain: 129/Sv.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13809369	E13291m	96T	$776		Add to Cart

ELISA Kit for Mouse Paired immunoglobulin-like type 2 receptor beta

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