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ELISA Kit for Mouse Lysine-specific histone demethylase 1A

SKU : BHE13806015

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EIAAB SCIENCE INC, WUHAN

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Mfr.No.	E12881m
Alternative Names	BRAF35-HDAC complex protein BHC110 ; Flavin-containing amine oxidase domain-containing protein 2 ; Aof2 ; Kiaa0601 ; Lsd1
Categories	Elisa
Function	Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation (By similarity). Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis.
Specificity	Natural and recombinant Mouse Lysine-specific histone demethylase 1A
Subcellular Location	Nucleus
Detection Range	0.312-20ng/mL
Entryname	KDM1A_MOUSE
Method Type	Sandwich
Platform	PF01593
Protein Name	Lysine-specific histone demethylase 1A
Sensitivity	0.15ng/mL
Sequence	50: MLSGKKAAAA ; AAAAAAAAAA ; GTEAGSGAAG ; GAENGSEVAA ; PPAGLTGPTD ; 100: MATGAAGERT ; PRKKEPPRAS ; PPGGLAEPPG ; SAGPQAGPTA ; GPGSATPMET ; 150: GIAETPEGRR ; TSRRKRAKVE ; YREMDESLAN ; LSEDEYYSEE ; ERNAKAEKEK ; 200: KLPPPPPQAP ; PEEENESEPE ; EPSGVEGAAF ; QSRLPHDRMT ; SQEAACFPDI ; 250: ISGPQQTQKV ; FLFIRNRTLQ ; LWLDNPKIQL ; TFEATLQQLE ; APYNSDTVLV ; 300: HRVHSYLERH ; GLINFGIYKR ; IKPLPIKKTG ; KVIIIGSGVS ; GLAAARQLQS ; 350: FGMDVTLLEA ; RDRVGGRVAT ; FRKGNYVADL ; GAMVVTGLGG ; NPMAVVSKQV ; 400: NMELAKIKQK ; CPLYEANGQA ; VPKEKDEMVE ; QEFNRLLEAT ; SYLSHQLDFN ; 450: VLNNKPVSLG ; QALEVVIQLQ ; EKHVKDEQIE ; HWKKIVKTQE ; ELKELLNKMV ; 500: NLKEKIKELH ; QQYKEASEVK ; PPRDITAEFL ; VKSKHRDLTA ; LCKEYDELAE ; 550: TQGKLEEKLQ ; ELEANPPSDV ; YLSSRDRQIL ; DWHFANLEFA ; NATPLSTLSL ; 600: KHWDQDDDFE ; FTGSHLTVRN ; GYSCVPVALA ; EGLDIKLNTA ; VRQVRYTASG ; 650: CEVIAVNTRS ; TSQTFIYKCD ; AVLCTLPLGV ; LKQQPPAVQF ; VPPLPEWKTS ; 700: AVQRMGFGNL ; NKVVLCFDRV ; FWDPSVNLFG ; HVGSTTASRG ; ELFLFWNLYK ; 750: APILLALVAG ; EAAGIMENIS ; DDVIVGRCLA ; ILKGIFGSSA ; VPQPKETVVS ; 800: RWRADPWARG ; SYSYVAAGSS ; GNDYDLMAQP ; ITPGPSIPGA ; PQPIPRLFFA ; 850: GEHTIRNYPA ; TVHGALLSGL ; REAGRIADQF ; LGAMYTLPRQ ; ATPGVPAQQS ; 853: PSM
Sequence Length	853
String	Q6ZQ88
Sub Unit	Component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B/BRAF35, KDM1A, RCOR1/CoREST and PHF21A/BHC80. The BHC complex may also contain ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. In the complex, RCOR1/CoREST strongly enhances the demethylase activity and protects it from the proteasome while PHF21A/BHC80 inhibits the demethylase activity. Interacts with the androgen receptor (AR).

Uniprot	Q6ZQ88
Gene Name	Kdm1a
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:99982
Unigene	Mm.28540

Reference	1. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H. DNA Res.10:167-180(2003) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] tissue: Brain. 2. "Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S., Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.more... PLoS Biol.7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] strain: C57BL/6J. 3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] strain: C57BL/6. tissue: Brain. tissue: Mammary gland. 4. "LSD1 demethylates repressive histone marks to promote androgen-receptor-dependent transcription." Metzger E., Wissmann M., Yin N., Mueller J.M., Schneider R., Peters A.H.F.M., Guenther T., Buettner R., Schuele R. Nature437:436-439(2005) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: TISSUE SPECIFICITY 5. "Epigenetic regulation of hematopoietic differentiation by Gfi-1 and Gfi-1b is mediated by the cofactors CoREST and LSD1." Saleque S., Kim J., Rooke H.M., Orkin S.H. Mol. Cell27:562-572(2007) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY AS A COMPONENT OF A GFI-RCOR-KDM1A-HDAC COMPLEX;INTERACTION WITH GFI1 AND GFI1B;FUNCTION 6. "The phagosomal proteome in interferon-gamma-activated macrophages." Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P. Immunity30:143-154(2009) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-167;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS] 7. "The lysine demethylase LSD1 (KDM1) is required for maintenance of global DNA methylation." Wang J., Hevi S., Kurash J.K., Lei H., Gay F., Bajko J., Su H., Sun W., Chang H., Xu G., Gaudet F., Li E., Chen T. Nat. Genet.41:125-129(2009) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;DEVELOPMENTAL STAGE 8. "Insm1 controls development of pituitary endocrine cells and requires a SNAG domain for function and for recruitment of histone-modifying factors." Welcker J.E., Hernandez-Miranda L.R., Paul F.E., Jia S., Ivanov A., Selbach M., Birchmeier C. Development140:4947-4958(2013) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH INSM1

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