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Home / Assay Kits / ELISA Kits

ELISA Kit for Mouse Hypoxia-inducible factor 1-alpha

SKU : BHE13805279

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EIAAB SCIENCE INC, WUHAN

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Mfr.No.	E0798m
Alternative Names	ARNT-interacting protein
Categories	Elisa
Function	Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions activates the transcription of over 40 genes, including, erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP.
Specificity	Natural and recombinant Mouse Hypoxia-inducible factor 1-alpha
Subcellular Location	Cytoplasm Nucleus Cytoplasmic in normoxia, nuclear translocation in response to hypoxia. Colocalizes with SUMO1 in the nucleus, under hypoxia.
Detection Range	78-5000pg/mL
Entryname	HIF1A_MOUSE
Method Type	Sandwich
Platform	PF11413
Protein Name	Hypoxia-inducible factor 1-alpha
Sensitivity	39.8pg/mL
Sequence	50: MEGAGGENEK ; KKMSSERRKE ; KSRDAARSRR ; SKESEVFYEL ; AHQLPLPHNV ; 100: SSHLDKASVM ; RLTISYLRVR ; KLLDAGGLDS ; EDEMKAQMDC ; FYLKALDGFV ; 150: MVLTDDGDMV ; YISDNVNKYM ; GLTQFELTGH ; SVFDFTHPCD ; HEEMREMLTH ; 200: RNGPVRKGKE ; LNTQRSFFLR ; MKCTLTSRGR ; TMNIKSATWK ; VLHCTGHIHV ; 250: YDTNSNQPQC ; GYKKPPMTCL ; VLICEPIPHP ; SNIEIPLDSK ; TFLSRHSLDM ; 300: KFSYCDERIT ; ELMGYEPEEL ; LGRSIYEYYH ; ALDSDHLTKT ; HHDMFTKGQV ; 350: TTGQYRMLAK ; RGGYVWVETQ ; ATVIYNTKNS ; QPQCIVCVNY ; VVSGIIQHDL ; 400: IFSLQQTESV ; LKPVESSDMK ; MTQLFTKVES ; EDTSCLFDKL ; KKEPDALTLL ; 450: APAAGDTIIS ; LDFGSDDTET ; EDQQLEDVPL ; YNDVMFPSSN ; EKLNINLAMS ; 500: PLPSSETPKP ; LRSSADPALN ; QEVALKLESS ; PESLGLSFTM ; PQIQDQPASP ; 550: SDGSTRQSSP ; ERLLQENVNT ; PNFSQPNSPS ; EYCFDVDSDM ; VNVFKLELVE ; 600: KLFAEDTEAK ; NPFSTQDTDL ; DLEMLAPYIP ; MDDDFQLRSF ; DQLSPLESNS ; 650: PSPPSMSTVT ; GFQQTQLQKP ; TITATATTTA ; TTDESKTETK ; DNKEDIKILI ; 700: ASPSSTQVPQ ; ETTTAKASAY ; SGTHSRTASP ; DRAGKRVIEQ ; TDKAHPRSLN ; 750: LSATLNQRNT ; VPEEELNPKT ; IASQNAQRKR ; KMEHDGSLFQ ; AAGIGTLLQQ ; 800: PGDCAPTMSL ; SWKRVKGFIS ; SEQNGTEQKT ; IILIPSDLAC ; RLLGQSMDES ; 836: GLPQLTSYDC ; EVNAPIQGSR ; NLLQGEELLR ; ALDQVN
Sequence Length	836
String	Q61221
Sub Unit	Interacts with the HIF1A beta/ARNT subunit; heterodimerization is required for DNA binding. Interacts with Interacts with NCOA1, NCOA2, APEX and HSP90. Interacts (hydroxylated within the ODD domain) with VHLL (via beta domain); the interaction, leads to polyubiquitination and subsequent HIF1A proteasomal degradation. During hypoxia, sumoylated HIF1A also binds VHL; the interaction promotes the ubiquitination of HIF1A. Interacts with SENP1; the interaction desumoylates HIF1A resulting in stabilization and activation of transcription. Interacts (Via the ODD domain) with ARD1A; the interaction appears not to acetylate HIF1A nor have any affect on protein stability, during hypoxia. Interacts with RWDD3; the interaction enhances HIF1A sumoylation. Interacts with RORA (via the DNA binding domain); the interaction enhances HIF1A transcription under hypoxia through increasing protein stability. Interaction with PSMA7 inhibits the transactivation activity of HIF1A under both normoxic and hypoxia-mimicking conditions. Interacts with USP20. Interacts with GNB2L1/RACK1; promotes HIF1A ubiquitination and proteasome-mediated degradation. Interacts with EP300 (via TAZ-type 1 domain); the interaction is stimulated in response to hypoxia and inhibited by CITED2. Interacts with CREBBP (via TAZ-type 1 domain) (By similarity). Interacts with COPS5; the interaction increases the transcriptional activity of HIF1A through increased stability. Interacts with TSGA10.

Uniprot	Q61221
Gene Name	Hif1a
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:15251
Unigene	Mm.3879

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SKU	Supplier No.	Size	Your price	Quantity
BHE13805279	E0798m	96T	$776		Add to Cart

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