ELISA Kit for Mouse Hyaluronan mediated motility receptor

Mfr.No.

E9486m

Alternative Names

Intracellular hyaluronic acid-binding protein ; Receptor for hyaluronan-mediated motility ; CD168 ; Ihabp ; Rhamm

Categories

Elisa

Function

Involved in cell motility. When hyaluronan binds to HMMR, the phosphorylation of a number of proteins, including the focal adhesion kinase occurs. May also be involved in cellular transformation and metastasis formation, and in regulating extracellular-regulated kinase (ERK) activity.

Specificity

Natural and recombinant Mouse Hyaluronan mediated motility receptor

Subcellular Location

Cell surface Cytoplasm

Detection Range

15.6-1000pg/mL

Entryname

HMMR_MOUSE

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	90-99%	92-101%	92-102%	92-106%
EDTA plasma(n=5)	92-98%	94-105%	90-101%	95-107%
heparin plasma(n=5)	94-104%	94-108%	101-107%	93-109%

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.5%

Protein Name

Hyaluronan mediated motility receptor

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-106
Plasma	99	90-107

Sensitivity

7.94pg/mL

Sequence

50:

MSFPKAPLKR ; FNDPSGCAPS ; PGAYDVKTSE ; ATKGPVSFQK ; SQRFKNQRES ;

100:

QQNLNIDKDT ; TLLASAKKAK ; KSVSKKDSQK ; NDKDVKRLEK ; EIRALLQERG ;

150:

TQDKRIQDME ; SELEKTEAKL ; NAAVREKTSL ; SASNASLEKR ; LTELTRANEL ;

200:

LKAKFSEDGH ; QKNMRALSLE ; LMKLRNKRET ; KMRSMMVKQE ; GMELKLQATQ ;

250:

KDLTESKGKI ; VQLEGKLVSI ; EKEKIDEKCE ; TEKLLEYIQE ; ISCASDQVEK ;

300:

CKVDIAQLEE ; DLKEKDREIL ; SLKQSLEENI ; TFSKQIEDLT ; VKCQLLETER ;

350:

DNLVSKDRER ; AETLSAEMQI ; LTERLALERQ ; EYEKLQQKEL ; QSQSLLQQEK ;

400:

ELSARLQQQL ; CSFQEEMTSE ; KNVFKEELKL ; ALAELDAVQQ ; KEEQSERLVK ;

450:

QLEEETKSTA ; EQLTRLDNLL ; REKEVELEKH ; IAAHAQAILI ; AQEKYNDTAQ ;

500:

SLRDVTAQLE ; SVQEKYNDTA ; QSLRDVTAQL ; ESEQEKYNDT ; AQSLRDVTAQ ;

550:

LESEQEKYND ; TAQSLRDVTA ; QLESVQEKYN ; DTAQSLRDVT ; AQLESYKSST ;

600:

LKEIEDLKLE ; NLTLQEKVAM ; AEKSVEDVQQ ; QILTAESTNQ ; EYARMVQDLQ ;

650:

NRSTLKEEEI ; KEITSSFLEK ; ITDLKNQLRQ ; QDEDFRKQLE ; EKGKRTAEKE ;

700:

NVMTELTMEI ; NKWRLLYEEL ; YEKTKPFQQQ ; LDAFEAEKQA ; LLNEHGATQE ;

750:

QLNKIRDSYA ; QLLGHQNLKQ ; KIKHVVKLKD ; ENSQLKSEVS ; KLRSQLVKRK ;

794:

QNELRLQGEL ; DKALGIRHFD ; PSKAFCHASK ; ENFTPLKEGN ; PNCC

Sequence Length

794

String

Q00547

Sub Unit

Subunit of the HARC complex.

Uniprot	Q00547
Gene Name	Hmmr
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:15366
Unigene	Mm.116997

Reference	1. "Identification of IHABP, a 95 kDa intracellular hyaluronate binding protein." Hofmann M., Fieber C., Assmann V., Goettlicher M., Sleeman J., Plug R., Howells N., von Stein O., Ponta H., Herrlich P. J. Cell Sci.111:1673-1684(1998) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];SUBCELLULAR LOCATION tissue: Lung. 2. Zhao Y., Zhang S., Turley E. Submitted (1998-07) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] 3. "Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S., Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.more... PLoS Biol.7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] strain: C57BL/6J. 4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (2005-07) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] 5. "Characterization of the murine gene encoding the intracellular hyaluronan receptor IHABP." Fieber C., Plug R., Sleeman J., Dall P., Ponta H., Hofmann M. Gene226:41-50(1999) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-183;TISSUE SPECIFICITY strain: 129/Sv. 6. "Characterization of the murine gene encoding the hyaluronan receptor RHAMM." Entwistle J., Zhang S., Yang B., Wong C., Li Q., Hall C.L., Jingbo A., Mowat M., Greenberg A.H., Turley E.A. Gene163:233-238(1995) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 164-794;ALTERNATIVE SPLICING strain: BALB/c. tissue: Fibroblast. 7. "Molecular cloning of a novel hyaluronan receptor that mediates tumor cell motility." Hardwick C., Hoare K., Owens R., Hohn H.P., Hook M., Moore D., Cripps V., Austen L., Nance D.M., Turley E.A. J. Cell Biol.117:1343-1350(1992) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 318-794;CHARACTERIZATION strain: BALB/c. 8. Hardwick C., Hoare K., Owens R., Hohn H.P., Hook M., Moore D., Cripps V., Austen L., Nance D.M., Turley E.A. J. Cell Biol.118:753-753(1992) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ERRATUM 9. "Hyaluronan and the hyaluronan receptor RHAMM promote focal adhesion turnover and transient tyrosine kinase activity." Hall C.L., Wang C., Lange L.A., Turley E.A. J. Cell Biol.126:575-588(1994) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: CHARACTERIZATION 10. "The hyaluronan receptor RHAMM regulates extracellular-regulated kinase." Zhang S., Chang M.C., Zylka D., Turley S., Harrison R., Turley E.A. J. Biol. Chem.273:11342-11348(1998) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ERK REGULATION;SUBCELLULAR LOCATION 11. "Problems with RHAMM: a new link between surface adhesion and oncogenesis?" Hofmann M., Assmann V., Fieber C., Sleeman J.P., Moll J., Ponta H., Hart I.R., Herrlich P. Cell95:591-592(1998) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW

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ELISA Kit for Mouse Hyaluronan mediated motility receptor

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