ELISA Kit for Mouse Heat shock factor protein 1

Mfr.No.

E0435m

Alternative Names

Heat shock transcription factor 1 ; HSTF 1

Categories

Elisa

Function

DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.

Specificity

Natural and recombinant Mouse Heat shock factor protein 1

Subcellular Location

Cytoplasm Nucleus Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules.

Detection Range

0.312-20ng/mL

Entryname

HSF1_MOUSE

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HSF1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	93-100%	91-104%	95-99%	92-102%
EDTA plasma(n=5)	90-103%	94-100%	94-102%	91-105%
heparin plasma(n=5)	96-99%	92-102%	90-106%	93-100%

Method Type

Sandwich

Platform

PF00447

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.5%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤11.3%

Protein Name

Heat shock factor protein 1

Recovery

Recovery was determined by spiking various levels of HSF1 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	97	90-99
Plasma	98	96-102

Sensitivity

0.158ng/mL

Sequence

50:

MDLAVGPGAA ; GPSNVPAFLT ; KLWTLVSDPD ; TDALICWSPS ; GNSFHVFDQG ;

100:

QFAKEVLPKY ; FKHNNMASFV ; RQLNMYGFRK ; VVHIEQGGLV ; KPERDDTEFQ ;

150:

HPCFLRGQEQ ; LLENIKRKVT ; SVSTLKSEDI ; KIRQDSVTRL ; LTDVQLMKGK ;

200:

QECMDSKLLA ; MKHENEALWR ; EVASLRQKHA ; QQQKVVNKLI ; QFLISLVQSN ;

250:

RILGVKRKIP ; LMLSDSNSAH ; SVPKYGRQYS ; LEHVHGPGPY ; SAPSPAYSSS ;

300:

SLYSSDAVTS ; SGPIISDITE ; LAPTSPLASP ; GRSIDERPLS ; SSTLVRVKQE ;

350:

PPSPPHSPRV ; LEASPGRPSS ; MDTPLSPTAF ; IDSILRESEP ; TPAASNTAPM ;

400:

DTTGAQAPAL ; PTPSTPEKCL ; SVACLDKNEL ; SDHLDAMDSN ; LDNLQTMLTS ;

450:

HGFSVDTSAL ; LDLFSPSVTM ; PDMSLPDLDS ; SLASIQELLS ; PQEPPRPIEA ;

500:

ENSNPDSGKQ ; LVHYTAQPLF ; LLDPDAVDTG ; SSELPVLFEL ; GESSYFSEGD ;

525:

DYTDDPTISL ; LTGTEPHKAK ; DPTVS

Sequence Length

525

String

P38532

Sub Unit

Monomer (By similarity). Under normal conditions, interacts with HSP90AA1 in the HSP90 multichaperone complex; the interaction prevents trimerization and activation of HSF1. On activation by heat-stress or by other factors such as metal ions, HSF1 is released from the complex, homotrimerizes, is hyperphosphorylated and translocated to the nucleus where, subsequently, it can activate transcription. Binds the complex through the regulatory domain. Interacts with SYMPK and CSTF2 in heat-stressed cells. Interacts with FKBP4 in the HSP90 multichaperone complex; the interaction is independent of the phosphorylation state of HSF1. Interacts with MAPKAPK2.

Uniprot	P38532
Gene Name	Hsf1
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:15499
Unigene	Mm.347444

Reference	1. "Cloning and characterization of two mouse heat shock factors with distinct inducible and constitutive DNA-binding ability." Sarge K.D., Zimarino V., Holm K., Wu C., Morimoto R.I. Genes Dev.5:1902-1911(1991) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1) strain: WEHI-3. 2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1) 3. "Structural organization and promoter analysis of murine heat shock transcription factor-1 gene." Zhang Y., Koushik S., Dai R., Mivechi N.F. J. Biol. Chem.273:32514-32521(1998) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 40-525 (ISOFORM 2) strain: 129/SvJ. tissue: Liver. 4. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry." Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J. Mol. Cell. Proteomics8:904-912(2009) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-303 AND SER-307;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS] tissue: Embryonic fibroblast.

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ELISA Kit for Mouse Heat shock factor protein 1

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