ELISA Kit for Mouse H-2 class II histocompatibility antigen gamma chain

Mfr.No.

E13627m

Alternative Names

Ia antigen-associated invariant chain ; Ii ; MHC class II-associated invariant chain ; CD74 ; Ii

Categories

Elisa

Function

Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to compartments where peptide loading of class II takes place.

Specificity

Natural and recombinant Mouse H-2 class II histocompatibility antigen gamma chain

Subcellular Location

Membrane Single-pass type II membrane protein

Detection Range

0.156-10ng/mL

Entryname

HG2A_MOUSE

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	89-99%	90-101%	93-101%	92-106%
EDTA plasma(n=5)	89-97%	90-102%	89-102%	90-106%
heparin plasma(n=5)	93-104%	94-108%	101-109%	93-110%

Method Type

Sandwich

Platform

PF09307

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤ 5.8%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤ 9.3%

Protein Name

H-2 class II histocompatibility antigen gamma chain

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	96	94-98
Plasma	97	93-99

Sensitivity

0.066ng/mL

Sequence

50:

MDDQRDLISN ; HEQLPILGNR ; PREPERCSRG ; ALYTGVSVLV ; ALLLAGQATT ;

100:

AYFLYQQQGR ; LDKLTITSQN ; LQLESLRMKL ; PKSAKPVSQM ; RMATPLLMRP ;

150:

MSMDNMLLGP ; VKNVTKYGNM ; TQDHVMHLLT ; RSGPLEYPQL ; KGTFPENLKH ;

200:

LKNSMDGVNW ; KIFESWMKQW ; LLFEMSKNSL ; EEKKPTEAPP ; KVLTKCQEEV ;

250:

SHIPAVYPGA ; FRPKCDENGN ; YLPLQCHGST ; GYCWCVFPNG ; TEVPHTKSRG ;

279:

RHNCSEPLDM ; EDLSSGLGVT ; RQELGQVTL

Sequence Length

279

String

P04441

Sub Unit

Nonamer composed of three alpha/beta/gamma heterotrimers.

Uniprot	P04441
Gene Name	Cd74
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:16149
Unigene	Mm.439737

Reference	1. "Primary structure of the gene for the murine Ia antigen-associated invariant chains (Ii). An alternatively spliced exon encodes a cysteine-rich domain highly homologous to a repetitive sequence of thyroglobulin." Koch N., Lauer W., Habicht J., Dobberstein B. EMBO J.6:1677-1683(1987) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM LONG);ALTERNATIVE SPLICING strain: AKR. 2. "Complete sequence of the murine invariant chain (Ii) gene." Zhu L., Jones P.P. Nucleic Acids Res.17:447-448(1989) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] strain: AKR. tissue: Liver. 3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG) tissue: Mammary gland. 4. "Nucleotide sequences of the murine Ia-associated invariant chain (Ii) and I-E (H-2S, Beta) chain expressible cDNA clones." Stone J., Perry R., Todd J.A., McDevitt H.O. Submitted (1988-03) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-58 tissue: Spleen. 5. "The IFN-gamma response of the murine invariant chain gene is mediated by a complex enhancer that includes several MHC class II consensus elements." Eades A.-M., Litfin M., Rahmsdorf H.J. J. Immunol.144:4399-4409(1990) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-25 6. "Structure of the murine Ia-associated invariant (Ii) chain as deduced from a cDNA clone." Singer P.A., Lauer W., Dembic Z., Mayer W.E., Lipp J., Koch N., Hammerling G., Klein J., Dobberstein B. EMBO J.3:873-877(1984) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 47-279 (ISOFORM SHORT) 7. "Identification of the glycosaminoglycan-attachment site of mouse invariant-chain proteoglycan core protein by site-directed mutagenesis." Miller J., Hatch J.A., Simonis S., Cullen S.E. Proc. Natl. Acad. Sci. U.S.A.85:1359-1363(1988) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION AT SER-265;MUTAGENESIS OF SER-265 8. "The phagosomal proteome in interferon-gamma-activated macrophages." Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P. Immunity30:143-154(2009) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-9;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]

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ELISA Kit for Mouse H-2 class II histocompatibility antigen gamma chain

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