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Home / Assay Kits / ELISA Kits

ELISA Kit for Mouse Flap endonuclease 1

SKU : BHE13804201

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EIAAB SCIENCE INC, WUHAN

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Mfr.No.	E15243m
Alternative Names	Flap structure-specific endonuclease 1 ; Fen-1
Categories	Elisa
Function	Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
Specificity	Natural and recombinant Mouse Flap endonuclease 1
Subcellular Location	Nucleus nucleolus Nucleus nucleoplasm Mitochondrion Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.
Detection Range	0.78-50ng/mL
Entryname	FEN1_MOUSE
Method Type	Sandwich
Platform	PF00867
Protein Name	Flap endonuclease 1
Sensitivity	0.27ng/mL
Sequence	50: MGIHGLAKLI ; ADVAPSAIRE ; NDIKSYFGRK ; VAIDASMSIY ; QFLIAVRQGG ; 100: DVLQNEEGET ; TSLMGMFYRT ; IRMENGIKPV ; YVFDGKPPQL ; KSGELAKRSE ; 150: RRAEAEKQLQ ; QAQEAGMEEE ; VEKFTKRLVK ; VTKQHNDECK ; HLLSLMGIPY ; 200: LDAPSEAEAS ; CAALAKAGKV ; YAAATEDMDC ; LTFGSPVLMR ; HLTASEAKKL ; 250: PIQEFHLSRV ; LQELGLNQEQ ; FVDLCILLGS ; DYCESIRGIG ; AKRAVDLIQK ; 300: HKSIEEIVRR ; LDPSKYPVPE ; NWLHKEAQQL ; FLEPEVVDPE ; SVELKWSEPN ; 350: EEELVKFMCG ; EKQFSEERIR ; SGVKRLSKSR ; QGSTQGRLDD ; FFKVTGSLSS ; 378: AKRKEPEPKG ; PAKKKAKTGG ; AGKFRRGK
Sequence Length	378
String	P39749
Sub Unit	Interacts with PCNA. Three molecules of Fen1 bind to one PCNA trimer with each molecule binding to one PCNA monomer. PCNA stimulates the nuclease activity without altering cleavage specificity. The C-terminal domain binds EP300. Can bind simultaneously to both PCNA and EP300. Interacts with DDX11.

Uniprot	P39749
Gene Name	Fen1
Immunogen Species	Mus musculus (Mouse)
Unigene	Mm.2952

Reference	1. "Functional domains within FEN-1 and RAD2 define a family of structure-specific endonucleases: implications for nucleotide excision repair." Harrington J.J., Lieber M.R. Genes Dev.8:1344-1355(1994) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];PROTEIN SEQUENCE OF 260-275 AND 331-352;FUNCTION strain: BALB/c. 2. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways." Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y. Mol. Cell50:919-930(2013) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-373;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS] tissue: Embryonic fibroblast.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13804201	E15243m	96T	$776		Add to Cart

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