ELISA Kit for Mouse Fibrillin-2

Mfr.No.

E8805m

Alternative Names

Fbn-2

Categories

Elisa

Function

Fibrillins are structural components of 10-12 nm extracellular calcium-binding microfibrils, which occur either in association with elastin or in elastin-free bundles. Fibrillin-2-containing microfibrils regulate the early process of elastic fiber assembly. Regulates osteoblast maturation by controlling TGF-beta bioavailability and calibrating TGF-beta and BMP levels, respectively.

Specificity

Natural and recombinant Mouse Fibrillin-2

Subcellular Location

Secreted extracellular space extracellular matrix

Entryname

FBN2_MOUSE

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

Method Type

Sandwich

Platform

PF07645

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

Protein Name

Fibrillin-2

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

Sequence

50:

MGRRRRLCLQ ; PYFVWLGCVA ; LWAQGTDGQP ; QPPPPKTLRP ; QPPPQQVRPA ;

100:

VAGSEGGFMG ; PEYRDEGAVA ; ASRVRRRGQQ ; EILRGPNVCG ; SRFHSYCCPG ;

150:

WKTLPGGNQC ; IVPICRNSCG ; DGFCSRPNMC ; TCSSGQISPT ; CGAKSIQQCS ;

200:

VRCMNGGTCA ; DDHCQCQKGY ; IGTYCGQPVC ; ENGCQNGGRC ; IGPNRCACVY ;

250:

GFTGPQCERD ; YRTGPCFTQV ; NNQMCQGQLT ; GIVCTKTLCC ; ATIGRAWGHP ;

300:

CEMCPAQPQP ; CRRGFIPNIR ; TGACQDVDEC ; QAIPGLCQGG ; NCINTVGSFE ;

350:

CRCPAGHKQS ; ETTQKCEDID ; ECSVIPGVCE ; TGDCSNTVGS ; YFCLCPRGFV ;

400:

TSTDGSRCID ; QRAGTCFSGL ; VNGRCAQELP ; GRMAKAQCCC ; EPGRCWSIGT ;

450:

IPEACPVRGS ; EEYRRLCLDG ; LPMGGIPGSS ; VSRPGGTGST ; GNGYGPGGTG ;

500:

FLPIPGDNGF ; SPGVGGAGVG ; AGGQGPIITG ; LTILNQTIDI ; CKHHANLCLN ;

550:

GRCIPTVSSY ; RCECNMGYKQ ; DANGDCIDVD ; ECTSNPCSNG ; DCVNTPGSYY ;

600:

CKCHAGFQRT ; PTKQACIDID ; ECIQNGVLCK ; NGRCVNTDGS ; FQCICNAGFE ;

650:

LTTDGKNCVD ; HDECTTTNMC ; LNGMCINEDG ; SFKCVCKPGF ; ILAPNGRYCT ;

700:

DVDECQTPGI ; CMNGHCINNE ; GSFRCDCPPG ; LAVGVDGRVC ; VDTHMRSTCY ;

750:

GEIKKGVCVR ; PFPGAVTKSE ; CCCANPDYGF ; GEPCQPCPAK ; NSAEFHGLCS ;

800:

SGIGITVDGR ; DINECALDPD ; ICANGICENL ; RGSYRCNCNS ; GYEPDASGRN ;

850:

CIDIDECLVN ; RLLCDNGLCR ; NTPGSYSCTC ; PPGYVFRTET ; ETCEDVNECE ;

900:

SNPCVNGACR ; NNLGSFHCEC ; SPGSKLSSTG ; LICIDSLKGT ; CWLNIQDNRC ;

950:

EVNINGATLK ; SECCATLGAA ; WGSPCERCEL ; DAACPRGFAR ; IKGVTCEDVN ;

1000:

ECEVFPGVCP ; NGRCVNSKGS ; FHCECPEGLT ; LDGTGRVCLD ; IRMEHCFLKW ;

1050:

DEDECIHPVP ; GKFRMDACCC ; AVGAAWGTEC ; EECPKPGTKE ; YETLCPRGPG ;

1100:

FANRGDILTG ; RPFYKDINEC ; KAFPGMCTYG ; KCRNTIGSFK ; CRCNNGFALD ;

1150:

MEERNCTDID ; ECRISPDLCG ; SGICVNTPGS ; FECECFEGYE ; SGFMMMKNCM ;

1200:

DIDECERNPL ; LCRGGTCVNT ; EGSFQCDCPL ; GHELSPSRED ; CVDINECSLS ;

1250:

DNLCRNGKCV ; NMIGTYQCSC ; NPGYQATPDR ; QGCTDIDECM ; IMNGGCDTQC ;

1300:

TNSEGSYECS ; CSEGYALMPD ; GRSCADIDEC ; ENNPDICDGG ; QCTNIPGEYR ;

1350:

CLCYDGFMAS ; MDMKTCIDVN ; ECDLNPNICM ; FGECENTKGS ; FICHCQLGYS ;

1400:

VKKGTTGCTD ; VDECEIGAHN ; CDMHASCLNV ; PGSFKCSCRE ; GWVGNGIKCI ;

1450:

DLDECANGTH ; QCSINAQCVN ; TPGSYRCACS ; EGFTGDGFTC ; SDVDECAENT ;

1500:

NLCENGQCLN ; VPGAYRCECE ; MGFTPASDSR ; SCQDIDECSF ; QNICVFGTCN ;

1550:

NLPGMFHCIC ; DDGYELDRTG ; GNCTDIDECA ; DPINCVNGLC ; VNTPGRYECN ;

1600:

CPPDFQLNPT ; GVGCVDNRVG ; NCYLKFGPRG ; DGSLSCNTEV ; GVGVSRSSCC ;

1650:

CSLGKAWGNP ; CETCPPVNST ; EYYTLCPGGE ; GFRPNPITII ; LEDIDECQEL ;

1700:

PGLCQGGNCI ; NTFGSFQCEC ; PQGYYLSEET ; RICEDIDECF ; AHPGVCGPGT ;

1750:

CYNTLGNYTC ; ICPPEYMQVN ; GGHNCMDMRK ; SFCYRSYNGT ; TCENELPFNV ;

1800:

TKRMCCCTYN ; VGKAWNKPCE ; PCPTPGTADF ; KTICGNIPGF ; TFDIHTGKAV ;

1850:

DIDECKEIPG ; ICANGVCINQ ; IGSFRCECPT ; GFSYNDLLLV ; CEDIDECSNG ;

1900:

DNLCQRNADC ; INSPGSYRCE ; CAAGFKLSPN ; GACVDRNECL ; EIPNVCSHGL ;

1950:

CVDLQGSYQC ; ICNNGFKASQ ; DQTMCMDVDE ; CERHPCGNGT ; CKNTVGSYNC ;

2000:

LCYPGFELTH ; NNDCLDIDEC ; SSFFGQVCRN ; GRCFNEIGSF ; KCLCNEGYEL ;

2050:

TPDGKNCIDT ; NECVALPGSC ; SPGTCQNLEG ; SFRCICPPGY ; EVRSENCIDI ;

2100:

NECDEDPNIC ; LFGSCTNTPG ; GFQCICPPGF ; VLSDNGRRCF ; DTRQSFCFTN ;

2150:

FENGKCSVPK ; AFNTTKAKCC ; CSKMPGEGWG ; DPCELCPKDD ; EVAFQDLCPY ;

2200:

GHGTVPSLHD ; TREDVNECLE ; SPGICSNGQC ; INTDGSFRCE ; CPMGYNLDYT ;

2250:

GVRCVDTDEC ; SIGNPCGNGT ; CTNVIGSFEC ; TCNEGFEPGP ; MMNCEDINEC ;

2300:

AQNPLLCAFR ; CMNTFGSYEC ; TCPVGYALRE ; DQKMCKDLDE ; CAEGLHDCES ;

2350:

RGMMCKNLIG ; TFMCICPPGM ; ARRPDGEGCV ; DENECRTKPG ; ICENGRCVNI ;

2400:

IGSYRCECNE ; GFQSSSSGTE ; CLDNRQGLCF ; AEVLQTMCQM ; ASSSRNLVTK ;

2450:

SECCCDGGRG ; WGHQCELCPL ; PGTAQYKKIC ; PHGPGYATDG ; RDIDECKVMP ;

2500:

SLCTNGQCVN ; TMGSFRCFCK ; VGYTTDISGT ; ACVDLDECSQ ; SPKPCNFICK ;

2550:

NTKGSYQCSC ; PRGYVLQEDG ; KTCKDLDECQ ; TKQHNCQFLC ; VNTLGGFTCK ;

2600:

CPPGFTQHHT ; ACIDNNECGS ; QPSLCGAKGI ; CQNTPGSFSC ; ECQRGFSLDA ;

2650:

SGLNCEDVDE ; CDGNHRCQHG ; CQNILGGYRC ; GCPQGYVQHY ; QWNQCVDENE ;

2700:

CSNPGACGSA ; SCYNTLGSYK ; CACPSGFSFD ; QFSSACHDVN ; ECSSSKNPCS ;

2750:

YGCSNTEGGY ; LCGCPPGYFR ; VGQGHCVSGM ; GFNKGQYLSV ; DAEAEDDENA ;

2800:

LSPEACYECK ; INGYTKKDGR ; RKRSAQEPEP ; ASAEEQISLE ; SVAMDSPVNM ;

2850:

KFNLSGLGSK ; EHILELVPAI ; EPLNNHIRYV ; ISQGNEDGVF ; RIHQRNGLSY ;

2900:

LHTAKKKLAP ; GTYTLEITSI ; PLYGKKELRK ; LEEHNEDDYL ; LGVLGEALRM ;

2907:

RLQIQLY

Sequence Length

2907

String

Q61555

Uniprot	Q61555
Gene Name	Fbn2
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:100047082
Unigene	Mm.20271

Reference	1. "Developmental expression of fibrillin genes suggests heterogeneity of extracellular microfibrils." Zhang H., Hu W., Ramirez F. J. Cell Biol.129:1165-1176(1995) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] 2. "Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S., Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.more... PLoS Biol.7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] strain: C57BL/6J. 3. "Fibrillin genes map to regions of conserved mouse/human synteny on mouse chromosomes 2 and 18." Li X., Pereira L., Zhang H., Sanguineti C., Ramirez F., Bonadio J., Francke U. Genomics18:667-672(1993) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 210-317 4. Lubec G., Sunyer B., Chen W.-Q. Submitted (2009-01) to the UniProtKB [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 2429-2441;IDENTIFICATION BY MASS SPECTROMETRY strain: OF1. tissue: Hippocampus. 5. "Fibrillin-1 and -2 differentially modulate endogenous TGF-{beta} and BMP bioavailability during bone formation." Nistala H., Lee-Arteaga S., Smaldone S., Siciliano G., Carta L., Ono R.N., Sengle G., Arteaga-Solis E., Levasseur R., Ducy P., Sakai L.Y., Karsenty G., Ramirez F. J. Cell Biol.190:1107-1121(2010) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;DISRUPTION PHENOTYPE

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ELISA Kit for Mouse Fibrillin-2

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