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Home / Assay Kits / ELISA Kits

ELISA Kit for Mouse E3 ubiquitin-protein ligase TRIM21

SKU : BHE13809828

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EIAAB SCIENCE INC, WUHAN

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Mfr.No.	E0290m
Alternative Names	52 kDa Ro protein ; 52 kDa ribonucleoprotein autoantigen Ro/SS-A ; RING-type E3 ubiquitin transferase TRIM21 ; Ro(SS-A) ; Sjoegren syndrome type A antigen ; SS-A ; Tripartite motif-containing protein 21 ; Ro52 ; Ssa1
Categories	Elisa
Function	E3 ubiquitin-protein ligase whose activity is dependent on E2 enzymes, UBE2D1, UBE2D2, UBE2E1 and UBE2E2. Forms a ubiquitin ligase complex in cooperation with the E2 UBE2D2 that is used not only for the ubiquitination of USP4 and IKBKB but also for its self-ubiquitination. Component of cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes such as SCF(SKP2)-like complexes. A TRIM21-containing SCF(SKP2)-like complex is shown to mediate ubiquitination of CDKN1B ('Thr-187' phosphorylated-form), thereby promoting its degradation by the proteasome. Monoubiquitinates IKBKB that will negatively regulates Tax-induced NF-kappa-B signaling. Negatively regulates IFN-beta production post-pathogen recognition by polyubiquitin-mediated degradation of IRF3. Mediates the ubiquitin-mediated proteasomal degradation of IgG1 heavy chain, which is linked to the VCP-mediated ER-associated degradation (ERAD) pathway. Promotes IRF8 ubiquitination, which enhanced the ability of IRF8 to stimulate cytokine genes transcription in macrophages. Plays a role in the regulation of the cell cycle progression. Enhances the decapping activity of DCP2. Exists as a ribonucleoprotein particle present in all mammalian cells studied and composed of a single polypeptide and one of four small RNA molecules. At least two isoforms are present in nucleated and red blood cells, and tissue specific differences in RO/SSA proteins have been identified. The common feature of these proteins is their ability to bind HY RNAs.2.
Specificity	Natural and recombinant Mouse E3 ubiquitin-protein ligase TRIM21
Subcellular Location	Cytoplasm Nucleus Cytoplasm P-body Enters the nucleus upon exposure to nitric oxide (By similarity). Localizes to small dot- or rod-like structures in the cytoplasm, called cytoplasmic bodies (P-body) that are located underneath the plasma membrane and also diffusely in the cytoplasm and are highly motil in cells. Cytoplasmic bodies are located along the microtubules and do not share the same cytoplasmic bodies with TRIM5. Colocalizes with DCP2 in P-body.
Detection Range	0.312-20ng/mL
Entryname	RO52_MOUSE
Method Type	Sandwich
Platform	PF13765
Protein Name	E3 ubiquitin-protein ligase TRIM21
Sensitivity	0.179ng/mL
Sequence	50: MSPSTTSKMS ; LEKMWEEVTC ; SICLDPMVEP ; MSIECGHCFC ; KECIFEVGKN ; 100: GGSSCPECRQ ; QFLLRNLRPN ; RHIANMVENL ; KQIAQNTKKS ; TQETHCMKHG ; 150: EKLHLFCEED ; GQALCWVCAQ ; SGKHRDHTRV ; PIEEAAKVYQ ; EKIHVVLEKL ; 200: RKGKELAEKM ; EMDLTMQRTD ; WKRNIDTQKS ; RIHAEFALQN ; SLLAQEEQRQ ; 250: LQRLEKDQRE ; YLRLLGKKEA ; ELAEKNQALQ ; ELISELERRI ; RGSELELLQE ; 300: VRIILERSGS ; WNLDTLDIDA ; PDLTSTCPVP ; GRKKMLRTCW ; VHITLDRNTA ; 350: NSWLIISKDR ; RQVRMGDTHQ ; NVSDNKERFS ; NYPMVLGAQR ; FSSGKMYWEV ; 400: DVTQKEAWDL ; GVCRDSVQRK ; GQFSLSPENG ; FWTIWLWQDS ; YEAGTSPQTT ; 450: LHIQVPPCQI ; GIFVDYEAGV ; VSFYNITDHG ; SLIYTFSECV ; FAGPLRPFFN ; 470: VGFNYSGGNA ; APLKLCPLKM ;
Sequence Length	470
String	Q62191
Sub Unit	Component of a SCF(SKP2)-like complex containing CUL1, SKP1, TRIM21 and SKP2. Interacts with CALR, CUL1, FBXW11, HSPA5, IKBKB, IRF3, SKP1 and VCP. Interacts with SKP2; the interaction with SKP2 does not depend on an intact F-box domain. Interacts (via N-terminus and C-terminus) with DCP2 (via N-terminus and C-terminus) (By similarity). Interacts (via C-terminus) with IRF8 (via C-terminus).

Uniprot	Q62191
Gene Name	Trim21
Immunogen Species	Mus musculus (Mouse)
Unigene	Mm.321227

Reference	1. "Structural differences between the human and mouse 52-kD Ro autoantigens associated with poorly conserved autoantibody activity across species." Keech C.L., Gordon T.P., McCluskey J. Clin. Exp. Immunol.104:255-263(1996) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] tissue: Macrophage. 2. "Autoantigen Ro52 is an interferon inducible E3 ligase that ubiquitinates IRF-8 and enhances cytokine expression in macrophages." Kong H.J., Anderson D.E., Lee C.H., Jang M.K., Tamura T., Tailor P., Cho H.K., Cheong J., Xiong H., Morse H.C. III, Ozato K. J. Immunol.179:26-30(2007) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 226-237; 242-252; 298-308 AND 329-340;FUNCTION;INTERACTION WITH IRF8;AUTOUBIQUITINATION;SUBCELLULAR LOCATION;INDUCTION;IDENTIFICATION BY MASS SPECTROMETRY

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SKU	Supplier No.	Size	Your price	Quantity
BHE13809828	E0290m	96T	$776		Add to Cart

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