ELISA Kit for Mouse Decorin

Mfr.No.

E6238m

Alternative Names

Bone proteoglycan II ; PG-S2 ; PG40

Categories

Elisa

Function

May affect the rate of fibrils formation.

Specificity

Natural and recombinant Mouse Decorin

Subcellular Location

Secreted extracellular space extracellular matrix

Detection Range

31.2-2000pg/mL

Entryname

PGS2_MOUSE

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PGS2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	96-110%	98-107%	95-109%	99-108%
Saliva(n=3)	94-108%	93-109%	97-106%	95-107%
heparin plasma(n=5)	93-111%	95-105%	94-110%	98-106%

Method Type

Sandwich

Platform

PF00560

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.1%

Protein Name

Decorin

Recovery

Recovery was determined by spiking various levels of PGS2 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	103	94-105
Plasma	101	93-106

Sensitivity

15.6pg/mL

Sequence

50:

MKATLIFFLL ; AQVSWAGPFE ; QRGLFDFMLE ; DEASGIIPYD ; PDNPLISMCP ;

100:

YRCQCHLRVV ; QCSDLGLDKV ; PWDFPPDTTL ; LDLQNNKITE ; IKEGAFKNLK ;

150:

DLHTLILVNN ; KISKISPEAF ; KPLVKLERLY ; LSKNQLKELP ; EKMPRTLQEL ;

200:

RVHENEITKL ; RKSDFNGLNN ; VLVIELGGNP ; LKNSGIENGA ; FQGLKSLSYI ;

250:

RISDTNITAI ; PQGLPTSLTE ; VHLDGNKITK ; VDAPSLKGLI ; NLSKLGLSFN ;

300:

SITVMENGSL ; ANVPHLRELH ; LDNNKLLRVP ; AGLAQHKYIQ ; VVYLHNNNIS ;

350:

AVGQNDFCRA ; GHPSRKASYS ; AVSLYGNPVR ; YWEIFPNTFR ; CVYVRSAIQL ;

354:

GNYK

Sequence Length

354

String

P28654

Sub Unit

Binds to type I and type II collagen, fibronectin and TGF-beta. Forms a ternary complex with MFAP2 and ELN. Interacts with DPT.

Uniprot	P28654
Gene Name	Dcn
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:13179
Unigene	Mm.56769

Reference	1. Naitoh Y., Suzuki S. Submitted (1990-07) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] strain: NIH Swiss. tissue: Fibroblast. 2. "The murine decorin. Complete cDNA cloning, genomic organization, chromosomal assignment, and expression during organogenesis and tissue differentiation." Scholzen T., Solursh M., Suzuki S., Reiter R., Morgan J.L., Buchberg A.M., Siracusa L.D., Iozzo R.V. J. Biol. Chem.269:28270-28281(1994) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]

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SKU	Supplier No.	Size	Your price	Quantity
BHE13808825	E6238m	96T	$776		Add to Cart

ELISA Kit for Mouse Decorin

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