This is a receptor for corticotropin releasing factor. Shows high-affinity CRF binding. Also binds to urocortin I, II and III. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
Specificity
Natural and recombinant Mouse Corticotropin-releasing factor receptor 2
Subcellular Location
Cell membrane Multi-pass membrane protein
Detection Range
0.312-20ng/mL
Entryname
CRFR2_MOUSE
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CRFR2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤3.7%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤6.5%
Protein Name
Corticotropin-releasing factor receptor 2
Recovery
Recovery was determined by spiking various levels of CRFR2 into serum and plasma .
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM CRF2-ALPHA)
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM CRF2-BETA)
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF 39-133 (ISOFORM CRF2-BETA);DISULFIDE BONDS
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF 39-133 (ISOFORM CRF2-BETA) IN COMPLEX WITH PEPTIDE LIGAND;DISULFIDE BONDS;MUTAGENESIS OF ASP-45
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF 39-133 (ISOFORM CRF2-BETA) IN COMPLEX WITH PEPTIDE LIGAND;DISULFIDE BONDS
Reviews of ELISA Kit for Mouse Corticotropin-releasing factor receptor 2