ELISA Kit for Mouse Beta-1,4-galactosyltransferase 5

Mfr.No.

E2493m

Alternative Names

Beta-1 ; 4-GalT II ; UDP-Gal:beta-GlcNAc beta-1 ; 4-galactosyltransferase 5 ; UDP-galactose:beta-N-acetylglucosamine beta-1 ; 4-galactosyltransferase 5 ; Bgt-5

Categories

Elisa

Function

Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.

Specificity

Natural and recombinant Mouse Beta-1,4-galactosyltransferase 5

Subcellular Location

Golgi apparatus Golgi stack membrane Single-pass type II membrane protein Trans cisternae of Golgi stack.

Detection Range

0.78-50ng/ml

Entryname

B4GT5_MOUSE

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of B4GT5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-109%	95-105%	97-104%	91-107%
Saliva(n=3)	94-106%	93-104%	92-106%	95-103%
heparin plasma(n=5)	91-108%	97-103%	96-104%	90-107%

Method Type

Competitive

Platform

PF02709

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.7%

Protein Name

Beta-1,4-galactosyltransferase 5

Recovery

Recovery was determined by spiking various levels of B4GT5 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	100	92-105
Plasma	99	95-103

Sensitivity

0.407ng/mL

Sequence

50:

MRARRGLLRL ; PRRSLLAALF ; FFSLSSSLLY ; FVYVAPGIVN ; TYLFMVQAQG ;

100:

ILLRDNVRTI ; GAQVYEQVVR ; SAYAKRNSSL ; NDSDYPLDLN ; HSEAFPPTTT ;

150:

FLPEDFTYFA ; NHPCPERLPS ; MKGPIDINMS ; EIAMDDIHEL ; FSRDPAIKLG ;

200:

GHWKPADCVP ; RWKVAILIPF ; RNRHEHLPVL ; LRHLLPMLQR ; QRLQFAFYVI ;

250:

EQVGTQPFNR ; AMLFNVGFQE ; AMKDLDWDCL ; IFHDVDHIPE ; SDRNYYGCGQ ;

300:

MPRHFATKLD ; KYMYLLPYTE ; FFGGVSGLTV ; EQFRKINGFP ; NAFWGWGGED ;

350:

DDLWNRVQNA ; GYSVSRPEGD ; TGKYKSIPHH ; HRGEVQFLGR ; YALLRKSKER ;

388:

QGLDGLNNLN ; YSANVTYDAL ; YKNITVNLTP ; ELAQVTEY

Sequence Length

388

String

Q9JMK0

Uniprot	Q9JMK0
Gene Name	B4galt5
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:56336
Unigene	Mm.200886

Reference	1. "Differential gene expression of beta-1,4-galactosyltransferases I, II and V during mouse brain development." Nakamura N., Yamakawa N., Sato T., Tojo H., Tachi C., Furukawa K. J. Neurochem.76:29-38(2001) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];TISSUE SPECIFICITY;DEVELOPMENTAL STAGE strain: BALB/c. tissue: Brain. 2. "Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S., Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.more... PLoS Biol.7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] strain: C57BL/6J. 3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (2005-07) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]

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ELISA Kit for Mouse Beta-1,4-galactosyltransferase 5

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