ELISA Kit for Mouse Baculoviral IAP repeat-containing protein 2

Mfr.No.

E10854m

Alternative Names

Cellular inhibitor of apoptosis 1 ; C-IAP1 ; Inhibitor of apoptosis protein 2 ; mIAP2 ; RING-type E3 ubiquitin transferase BIRC2

Categories

Elisa

Function

Apoptotic suppressor. The BIR motifs region interacts with TNF receptor associated factors 1 and 2 (TRAF1 and TRAF2) to form an heteromeric complex, which is then recruited to the tumor necrosis factor receptor 2 (TNFR2).

Specificity

Natural and recombinant Mouse Baculoviral IAP repeat-containing protein 2

Subcellular Location

Cytoplasm

Detection Range

0.156-10ng/mL

Entryname

BIRC2_MOUSE

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BIRC2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-99%	90-102%	91-101%	88-103%
EDTA plasma(n=5)	90-105%	89-107%	92-101%	95-99%
heparin plasma(n=5)	93-100%	94-102%	91-103%	91-105%

Method Type

Sandwich

Platform

PF00653

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.4%

Protein Name

Baculoviral IAP repeat-containing protein 2

Recovery

Recovery was determined by spiking various levels of BIRC2 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	95	91-101
Plasma	97	93-104

Sensitivity

0.086ng/mL

Sequence

50:

MDKTVSQRLG ; QGTLHQKLKR ; IMEKSTILSN ; WTKESEEKMK ; FDFSCELYRM ;

100:

STYSAFPRGV ; PVSERSLARA ; GFYYTGVNDK ; VKCFCCGLML ; DNWKQGDSPV ;

150:

EKHRQFYPSC ; SFVQTLLSAS ; LQSPSKNMSP ; VKSRFAHSSP ; LERGGIHSNL ;

200:

CSSPLNSRAV ; EDFSSRMDPC ; SYAMSTEEAR ; FLTYSMWPLS ; FLSPAELARA ;

250:

GFYYIGPGDR ; VACFACGGKL ; SNWEPKDDAM ; SEHRRHFPHC ; PFLENTSETQ ;

300:

RFSISNLSMQ ; THSARLRTFL ; YWPPSVPVQP ; EQLASAGFYY ; VDRNDDVKCF ;

350:

CCDGGLRCWE ; PGDDPWIEHA ; KWFPRCEFLI ; RMKGQEFVDE ; IQARYPHLLE ;

400:

QLLSTSDTPG ; EENADPTETV ; VHFGPGESSE ; DVVMMSTPVV ; KAALEMGFSR ;

450:

SLVRQTVQRQ ; ILATGENYRT ; VNDIVSVLLN ; AEDERREEEK ; ERQTEEMASG ;

500:

DLSLIRKNRM ; ALFQQLTHVL ; PILDNLLEAS ; VITKQEHDII ; RQKTQIPLQA ;

550:

RELIDTVLVK ; GNAAANIFKN ; SLKEIDSTLY ; ENLFVEKNMK ; YIPTEDVSGL ;

600:

SLEEQLRRLQ ; EERTCKVCMD ; REVSIVFIPC ; GHLVVCQECA ; PSLRKCPICR ;

612:

GTIKGTVRTF ; LS

Sequence Length

612

String

Q62210

Sub Unit

Interacts with DIABLO/SMAC and with PRSS25; these interactions inhibit apoptotic suppressor activity. Interacts with CASP9.

Uniprot	Q62210
Gene Name	Birc2
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:11797
Unigene	Mm.335659

Reference	1. "The TNFR2-TRAF signaling complex contains two novel proteins related to baculoviral inhibitor of apoptosis proteins." Rothe M., Pan M.-G., Henzel W.J., Ayres T.M., Goeddel D.V. Cell83:1243-1252(1995) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];PARTIAL PROTEIN SEQUENCE 2. "Genomic characterization of the mouse inhibitor of apoptosis protein 1 and 2 genes." Liston P., Lefebvre C., Fong W.G., Xuan J.Y., Korneluk R.G. Genomics46:495-503(1997) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] tissue: Skeletal muscle. 3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] tissue: Brain. 4. "c-IAP1 and c-IAP2 are critical mediators of tumor necrosis factor alpha (TNFalpha)-induced NF-kappaB activation." Varfolomeev E., Goncharov T., Fedorova A.V., Dynek J.N., Zobel K., Deshayes K., Fairbrother W.J., Vucic D. J. Biol. Chem.283:24295-24299(2008) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION AS AN E3 UBIQUITIN-PROTEIN LIGASE

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE13801114	E10854m	96T	$776		Add to Cart

ELISA Kit for Mouse Baculoviral IAP repeat-containing protein 2

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of ELISA Kit for Mouse Baculoviral IAP repeat-containing protein 2

Rate it now..!!