ELISA Kit for Mouse Angiotensin-converting enzyme

Mfr.No.

E0004m

Alternative Names

Dipeptidyl carboxypeptidase I ; Kininase II ; CD143 ; Dcp1

Categories

Elisa

Function

Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. This GPIase activity seems to be crucial for the egg-binding ability of the sperm.

Specificity

Natural and recombinant Mouse Angiotensin-converting enzyme

Subcellular Location

Cell membrane Single-pass type I membrane protein

Detection Range

78-5000pg/mL

Entryname

ACE_MOUSE

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
Saliva(n=3)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

Method Type

Sandwich

Platform

PF01401

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

Protein Name

Angiotensin-converting enzyme

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

Sensitivity

39pg/mL

Sequence

50:

MGAASGQRGR ; WPLSPPLLML ; SLLVLLLQPS ; PAPALDPGLQ ; PGNFSPDEAG ;

100:

AQLFAESYNS ; SAEVVMFQST ; VASWAHDTNI ; TEENARRQEE ; AALVSQEFAE ;

150:

VWGKKAKELY ; ESIWQNFTDS ; KLRRIIGSIR ; TLGPANLPLA ; QRQQYNSLLS ;

200:

NMSRIYSTGK ; VCFPNKTATC ; WSLDPELTNI ; LASSRSYAKL ; LFAWEGWHDA ;

250:

VGIPLKPLYQ ; DFTAISNEAY ; RQDDFSDTGA ; FWRSWYESPS ; FEESLEHIYH ;

300:

QLEPLYLNLH ; AYVRRALHRR ; YGDKYVNLRG ; PIPAHLLGDM ; WAQSWENIYD ;

350:

MVVPFPDKPN ; LDVTSTMVQK ; GWNATHMFRV ; SEEFFTSLGL ; SPMPPEFWAE ;

400:

SMLEKPTDGR ; EVVCHASAWD ; FYNRKDFRIK ; QCTRVTMEQL ; ATVHHEMGHV ;

450:

QYYLQYKDLH ; VSLRRGANPG ; FHEAIGDVLA ; LSVSTPAHLH ; KIGLLDHVTN ;

500:

DIESDINYLL ; KMALEKIAFL ; PFGYLVDQWR ; WGVFSGRTPP ; SRYNFDWWYL ;

550:

RTKYQGICPP ; VARNETHFDA ; GAKFHIPNVT ; PYIRYFVSFV ; LQFQFHQALC ;

600:

KEAGHQGPLH ; QCDIYQSAQA ; GAKLKQVLQA ; GCSRPWQEVL ; KDLVGSDALD ;

650:

AKALLEYFQP ; VSQWLEEQNQ ; RNGEVLGWPE ; NQWRPPLPDN ; YPEGIDLETD ;

700:

EAKADRFVEE ; YDRTAQVLLN ; EYAEANWQYN ; TNITIEGSKI ; LLEKSTEVSN ;

750:

HTLKYGTRAK ; TFDVSNFQNS ; SIKRIIKKLQ ; NLDRAVLPPK ; ELEEYNQILL ;

800:

DMETTYSLSN ; ICYTNGTCMP ; LEPDLTNMMA ; TSRKYEELLW ; AWKSWRDKVG ;

850:

RAILPFFPKY ; VEFSNKIAKL ; NGYTDAGDSW ; RSLYESDNLE ; QDLEKLYQEL ;

900:

QPLYLNLHAY ; VRRSLHRHYG ; SEYINLDGPI ; PAHLLGNMWA ; QTWSNIYDLV ;

950:

APFPSAPNID ; ATEAMIKQGW ; TPRRIFKEAD ; NFFTSLGLLP ; VPPEFWNKSM ;

1000:

LEKPTDGREV ; VCHPSAWDFY ; NGKDFRIKQC ; TSVNMEDLVI ; AHHEMGHIQY ;

1050:

FMQYKDLPVT ; FREGANPGFH ; EAIGDIMALS ; VSTPKHLYSL ; NLLSTEGSGY ;

1100:

EYDINFLMKM ; ALDKIAFIPF ; SYLIDQWRWR ; VFDGSITKEN ; YNQEWWSLRL ;

1150:

KYQGLCPPVP ; RSQGDFDPGS ; KFHVPANVPY ; VRYFVSFIIQ ; FQFHEALCRA ;

1200:

AGHTGPLHKC ; DIYQSKEAGK ; LLADAMKLGY ; SKPWPEAMKL ; ITGQPNMSAS ;

1250:

AMMNYFKPLT ; EWLVTENRRH ; GETLGWPEYN ; WAPNTARAEG ; STAESNRVNF ;

1300:

LGLYLEPQQA ; RVGQWVLLFL ; GVALLVATVG ; LAHRLYNIRN ; HHSLRRPHRG ;

1312:

PQFGSEVELR ; HS

Sequence Length

1312

String

P09470

Uniprot	P09470
Gene Name	Ace
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:11421
Unigene	Mm.754

Reference	1. "Mouse angiotensin-converting enzyme is a protein composed of two homologous domains." Bernstein K.E., Martin B.M., Edwards A.S., Bernstein E.A. J. Biol. Chem.264:11945-11951(1989) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SOMATIC) 2. "Transcription of testicular angiotensin-converting enzyme (ACE) is initiated within the 12th intron of the somatic ACE gene." Howard T.E., Shai S.-Y., Langford K.G., Martin B.M., Bernstein K.E. Mol. Cell. Biol.10:4294-4302(1990) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM TESTIS-SPECIFIC);PARTIAL NUCLEOTIDE SEQUENCE [GENOMIC DNA] 3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SOMATIC) strain: FVB/N. tissue: Mammary gland. 4. "The isolation of angiotensin-converting enzyme cDNA." Bernstein K.E., Martin B.M., Bernstein E.A., Linton J., Striker L., Striker G. J. Biol. Chem.263:11021-11024(1988) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-332 (ISOFORM SOMATIC);PARTIAL PROTEIN SEQUENCE 5. "Partial protein sequence of mouse and bovine kidney angiotensin converting enzyme." Bernstein K.E., Martin B.M., Striker L., Striker G. Kidney Int.33:652-655(1988) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 35-54 tissue: Kidney. 6. "Male-female differences in fertility and blood pressure in ACE-deficient mice." Krege J.H., John S.W., Langenbach L.L., Hodgin J.B., Hagaman J.R., Bachman E.S., Jennette J.C., O'Brien D.A., Smithies O. Nature375:146-148(1995) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION 7. "Mice lacking angiotensin-converting enzyme have low blood pressure, renal pathology, and reduced male fertility." Esther C.R. Jr., Howard T.E., Marino E.M., Goddard J.M., Capecchi M.R., Bernstein K.E. Lab. Invest.74:953-965(1996) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;DISRUPTION PHENOTYPE 8. "Angiotensin-converting enzyme is a GPI-anchored protein releasing factor crucial for fertilization." Kondoh G., Tojo H., Nakatani Y., Komazawa N., Murata C., Yamagata K., Maeda Y., Kinoshita T., Okabe M., Taguchi R., Takeda J. Nat. Med.11:160-166(2005) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;ENZYME REGULATION;MUTAGENESIS OF HIS-993; GLU-994 AND HIS-997 9. "Proteome-wide characterization of N-glycosylation events by diagonal chromatography." Ghesquiere B., Van Damme J., Martens L., Vandekerckhove J., Gevaert K. J. Proteome Res.5:2438-2447(2006) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-151 strain: C57BL/6. tissue: Plasma. 10. "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M. Mol. Cell. Proteomics6:283-293(2007) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS] tissue: Brain cortex. 11. "Angiotensin II regulates ACE and ACE2 in neurons through p38 mitogen-activated protein kinase and extracellular signal-regulated kinase 1/2 signaling." Xiao L., Haack K.K., Zucker I.H. Am. J. Physiol.304:C1073-C1079(2013) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE13800730	E0004m	96T	$776		Add to Cart

ELISA Kit for Mouse Angiotensin-converting enzyme

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of ELISA Kit for Mouse Angiotensin-converting enzyme

Rate it now..!!