ELISA Kit for Human Retinoic acid receptor gamma

Mfr.No.

E10643h

Alternative Names

Nuclear receptor subfamily 1 group B member 3 ; NR1B3

Categories

Elisa

Function

Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, acts mainly as an activator of gene expression due to weak binding to corepressors. Required for limb bud development. In concert with RARA or RARB, required for skeletal growth, matrix homeostasis and growth plate function.

Specificity

Natural and recombinant Human Retinoic acid receptor gamma

Subcellular Location

Nucleus

Detection Range

78-5000pg/mL

Entryname

RARG_HUMAN

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of RARG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	90-99%	90-102%	88-103%	92-98%
EDTA plasma(n=5)	89-106%	93-100%	90-105%	92-101%
heparin plasma(n=5)	93-103%	94-102%	91-103%	95-100%

Method Type

Sandwich

Platform

PF00104

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.7%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.1%

Protein Name

Retinoic acid receptor gamma

Recovery

Recovery was determined by spiking various levels of RARG into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	95	91-103
Plasma	97	93-104

Sensitivity

39.16pg/mL

Sequence

50:

MATNKERLFA ; AGALGPGSGY ; PGAGFPFAFP ; GALRGSPPFE ; MLSPSFRGLG ;

100:

QPDLPKEMAS ; LSVETQSTSS ; EEMVPSSPSP ; PPPPRVYKPC ; FVCNDKSSGY ;

150:

HYGVSSCEGC ; KGFFRRSIQK ; NMVYTCHRDK ; NCIINKVTRN ; RCQYCRLQKC ;

200:

FEVGMSKEAV ; RNDRNKKKKE ; VKEEGSPDSY ; ELSPQLEELI ; TKVSKAHQET ;

250:

FPSLCQLGKY ; TTNSSADHRV ; QLDLGLWDKF ; SELATKCIIK ; IVEFAKRLPG ;

300:

FTGLSIADQI ; TLLKAACLDI ; LMLRICTRYT ; PEQDTMTFSD ; GLTLNRTQMH ;

350:

NAGFGPLTDL ; VFAFAGQLLP ; LEMDDTETGL ; LSAICLICGD ; RMDLEEPEKV ;

400:

DKLQEPLLEA ; LRLYARRRRP ; SQPYMFPRML ; MKITDLRGIS ; TKGAERAITL ;

450:

KMEIPGPMPP ; LIREMLENPE ; MFEDDSSQPG ; PHPNASSEDE ; VPGGQGKGGL ;

454:

KSPA

Sequence Length

454

String

P13631

Sub Unit

Homodimer (By similarity). Heterodimer with a RXR molecule (By similarity). Binds DNA preferentially as a RAR/RXR heterodimer (By similarity). Forms a complex with PUS1 and the SRA1 RNA in the nucleus.

Uniprot	P13631
Gene Name	RARG
Immunogen Species	Homo Sapiens (Human)
Kegg	hsa:5916
Unigene	Hs.1497

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SKU	Supplier No.	Size	Your price	Quantity
BHE13809166	E10643h	96T	$736		Add to Cart

ELISA Kit for Human Retinoic acid receptor gamma

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