CD85 antigen-like family member D ; Immunoglobulin-like transcript 4 ; ILT-4 ; Monocyte/macrophage immunoglobulin-like receptor 10 ; MIR-10 ; CD85d ; ILT4 ; LIR2 ; MIR10
Categories
Elisa
Function
Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Involved in the down-regulation of the immune response and the development of tolerance. Competes with CD8A for binding to class I MHC antigens. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.
Specificity
Natural and recombinant Human Leukocyte immunoglobulin-like receptor subfamily B member 2
Subcellular Location
Membrane Single-pass type I membrane protein
Detection Range
0.156-10ng/mL
Entryname
LIRB2_HUMAN
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
92-105%
90-101%
95-105%
92-109%
EDTA plasma(n=5)
89-95%
93105%
89-94%
90-106%
heparin plasma(n=5)
93-104%
94-110%
101-111%
91-110%
Method Type
Sandwich
Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤3.9%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.7%
Protein Name
Leukocyte immunoglobulin-like receptor subfamily B member 2
Recovery
Recovery was determined by spiking various levels of ACE into serum and plasma .