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ELISA Kit for Human HLA class II histocompatibility antigen, DRB1-9 beta chain

SKU : BHE13800295

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EIAAB SCIENCE INC, WUHAN

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Mfr.No.	E15037h
Antigen	HLA-DRB1
Alternative Names	MHC class II antigen DRB1*9 ; DR-9
Categories	Elisa
Function	Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accomodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route; where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules; and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments; exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides; autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs; other cells of the gastrointestinal tract; such as epithelial cells; express MHC class II molecules and CD74 and act as APCs; which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen; three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form an heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs; CD74 undergoes a sequential degradation by various proteases; including CTSS and CTSL; leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B cells; the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules; increased acidification produces increased proteolysis and efficient peptide loading.
Specificity	Natural and recombinant Human HLA class II histocompatibility antigen, DRB1-9 beta chain
Subcellular Location	Cell membrane Single-pass type I membrane protein Endoplasmic reticulum membrane Single-pass type I membrane protein Golgi apparatus trans-Golgi network membrane Single-pass type I membrane protein Endosome membrane Single-pass type I membrane protein Lysosome membrane Single-pass type I membrane protein Late endosome membrane Single-pass type I membrane protein The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation.
Detection Range	0.625-40ng/ml
Entryname	2B19_HUMAN
Method Type	Sandwich
Platform	PF07654
Protein Name	HLA class II histocompatibility antigen, DRB1-9 beta chain
Sensitivity	0.322ng/mL
Sequence	50: MVCLKLPGGS ; CMAALTVTLM ; VLSSPLALAG ; DTQPRFLKQD ; KFECHFFNGT ; 100: ERVRYLHRGI ; YNQEENVRFD ; SDVGEYRAVT ; ELGRPVAESW ; NSQKDFLERR ; 150: RAEVDTVCRH ; NYGVGESFTV ; QRRVHPEVTV ; YPAKTQPLQH ; HNLLVCSVSG ; 200: FYPGSIEVRW ; FRNGQEEKAG ; VVSTGLIQNG ; DWTFQTLVML ; ETVPRSGEVY ; 250: TCQVEHPSVM ; SPLTVEWRAR ; SESAQSKMLS ; GVGGFVLGLL ; FLGAGLFIYF ; 266: RNQKGHSGLQ ; PTGFLS
Sequence Length	266
String	Q9TQE0
Sub Unit	Heterodimer of an alpha and a beta subunit; also referred as MHC class II molecule. In the endoplasmic reticulum (ER) it forms an heterononamer; 3 MHC class II molecules bind to a CD74 homotrimer (also known as invariant chain or HLA class II histocompatibility antigen gamma chain). In the endosomal/lysosomal system; CD74 undergoes sequential degradation by various proteases; leaving a small fragment termed CLIP on each MHC class II molecule. MHC class II molecule interacts with HLA_DM, and HLA_DO in B cells, in order to release CLIP and facilitate the binding of antigenic peptides.

Uniprot	Q9TQE0
Gene Name	HLA-DRB1
Kegg	hsa:100294468
Unigene	Hs.534322

Reference	1. *"Complete cDNA sequence of the HLA-DRB109012 allele." Martinez-Quiles N., Martin-Villa J.M., Ferre-Lopez S., Moreno-Pelayo M.A., Martinez-Laso J., Perez-Blas M., Alegre R., Arnaiz-Villena A. Eur. J. Immunogenet.25:307-309(1998) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for*: NUCLEOTIDE SEQUENCE [MRNA] (ALLELE DRB109:01) tissue: Blood. 2. "Allelic variation in the DR subregion of the human major histocompatibility complex." Bell J.I., Denney D. Jr., Foster L., Belt T.K., Todd J.A., McDevitt H.O. Proc. Natl. Acad. Sci. U.S.A.84:6234-6238(1987) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 30-115 (ALLELE DRB109:01) 3. "Allelic diversity is generated by intraexon sequence exchange at the DRB1 locus of primates."* Gyllensten U.B., Sundvall M., Erlich H.A. Proc. Natl. Acad. Sci. U.S.A.88:3686-3690(1991) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 30-115 4. "Polymorphism of HLA-DR beta chains in DR4, -7, and -9 haplotypes: implications for the mechanisms of allelic variation." Gregersen P.K., Moriuchi T., Karr R.W., Obata F., Moriuchi J., Maccari J., Goldberg D., Winchester R.J., Silver J. Proc. Natl. Acad. Sci. U.S.A.83:9149-9153(1986) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 45-266 5. "Invariant chain structure and MHC class II function." Cresswell P. Cell84:505-507(1996) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW 6. "Presentation of antigens by MHC class II molecules: getting the most out of them." Villadangos J.A. Mol. Immunol.38:329-346(2001) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW 7. "Autophagy in MHC class II presentation: sampling from within." Menendez-Benito V., Neefjes J. Immunity26:1-3(2007) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW 8. "MHC class II molecules on the move for successful antigen presentation." Rocha N., Neefjes J. EMBO J.27:1-5(2008) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW 9. "MHC class II stabilization at the surface of human dendritic cells is the result of maturation-dependent MARCH I down-regulation." De Gassart A., Camosseto V., Thibodeau J., Ceppi M., Catalan N., Pierre P., Gatti E. Proc. Natl. Acad. Sci. U.S.A.105:3491-3496(2008) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: UBIQUITINATION BY MARCH1;SUBCELLULAR LOCATION 10. "MHC class II transport at a glance." Berger A.C., Roche P.A. J. Cell Sci.122:1-4(2009) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW 11. "CD74 in antigen presentation, inflammation, and cancers of the gastrointestinal tract." Beswick E.J., Reyes V.E. World J. Gastroenterol.15:2855-2861(2009) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW

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