ELISA Kit for Human Glutathione peroxidase 3

Mfr.No.

E9949h

Alternative Names

Extracellular glutathione peroxidase ; Plasma glutathione peroxidase ; GPx-P ; GPXP

Categories

Elisa

Function

Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione.

Specificity

Natural and recombinant Human Glutathione peroxidase 3

Subcellular Location

Secreted

Detection Range

1.56-100ng/mL

Entryname

GPX3_HUMAN

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Gpx3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	93-103%	92-104%	90-105%	92-102%
EDTA plasma(n=5)	90-106%	93-105%	92-104%	91-105%
heparin plasma(n=5)	95-103%	94-103%	91-107%	94-101%

Method Type

Competitive

Platform

PF00255

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.4%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.9%

Protein Name

Glutathione peroxidase 3

Recovery

Recovery was determined by spiking various levels of Gpx3 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	97	92-103
Plasma	98	93-105

Sensitivity

0.23ng/mL

Sequence

50:

MARLLQASCL ; LSLLLAGFVS ; QSRGQEKSKM ; DCHGGISGTI ; YEYGALTIDG ;

100:

EEYIPFKQYA ; GKYVLFVNVA ; SYUGLTGQYI ; ELNALQEELA ; PFGLVILGFP ;

150:

CNQFGKQEPG ; ENSEILPTLK ; YVRPGGGFVP ; NFQLFEKGDV ; NGEKEQKFYT ;

200:

FLKNSCPPTS ; ELLGTSDRLF ; WEPMKVHDIR ; WNFEKFLVGP ; DGIPIMRWHH ;

226:

RTTVSNVKMD ; ILSYMRRQAA ; LGVKRK

Sequence Length

226

String

P22352

Sub Unit

Homotetramer.

Uniprot	P22352
Gene Name	GPX3
Immunogen Species	Homo Sapiens (Human)
Kegg	hsa:2878
Unigene	Hs.386793

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SKU	Supplier No.	Size	Your price	Quantity
BHE13804861	E9949h	96T	$736		Add to Cart

ELISA Kit for Human Glutathione peroxidase 3

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