ELISA Kit for Human Erythropoietin

Mfr.No.

E0028h

Categories

Elisa

Function

Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass.

Specificity

Natural and recombinant Human Erythropoietin

Subcellular Location

Secreted

Detection Range

0.156-10ng/mL

Entryname

EPO_HUMAN

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of EPO and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	96-100%	90-98%	95-102%	92-97%
EDTA plasma(n=5)	89-95%	93-98%	99-105%	90-97%
heparin plasma(n=5)	93-98%	99-105%	101-107%	96-105%

Method Type

Sandwich

Platform

PF00758

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.5%

Protein Name

Erythropoietin

Recovery

Recovery was determined by spiking various levels of EPO into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	96	94-99
Plasma	98	97-102

Sensitivity

0.1ng/mL

Sequence

50:

MGVHECPAWL ; WLLLSLLSLP ; LGLPVLGAPP ; RLICDSRVLE ; RYLLEAKEAE ;

100:

NITTGCAEHC ; SLNENITVPD ; TKVNFYAWKR ; MEVGQQAVEV ; WQGLALLSEA ;

150:

VLRGQALLVN ; SSQPWEPLQL ; HVDKAVSGLR ; SLTTLLRALG ; AQKEAISPPD ;

193:

AASAAPLRTI ; TADTFRKLFR ; VYSNFLRGKL ; KLYTGEACRT ; GDR

Sequence Length

193

String

P01588

Uniprot	P01588
Gene Name	EPO
Immunogen Species	Homo Sapiens (Human)
Kegg	hsa:2056
Unigene	Hs.2303

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SKU	Supplier No.	Size	Your price	Quantity
BHE13803543	E0028h	96T	$536		Add to Cart

ELISA Kit for Human Erythropoietin

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