ELISA Kit for Human Cystathionine beta-synthase

Mfr.No.

E1957h

Alternative Names

Beta-thionase ; Serine sulfhydrase

Categories

Elisa

Function

Only known pyridoxal phosphate-dependent enzyme that contains heme. Important regulator of hydrogen sulfide, especially in the brain, utilizing cysteine instead of serine to catalyze the formation of hydrogen sulfide. Hydrogen sulfide is a gastratransmitter with signaling and cytoprotective effects such as acting as a neuromodulator in the brain to protect neurons against hypoxic injury.

Specificity

Natural and recombinant Human Cystathionine beta-synthase

Subcellular Location

Cytoplasm Nucleus

Detection Range

1.56-100ng/mL

Entryname

CBS_HUMAN

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CBS and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	95-107%	94-109%	97-104%	99-103%
Saliva(n=3)	93-105%	96-103%	97-104%	95-106%
heparin plasma(n=5)	98-108%	94-110%	99-106%	96-109%

Method Type

Sandwich

Platform

PF00571

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.5%

Protein Name

Cystathionine beta-synthase

Recovery

Recovery was determined by spiking various levels of CBS into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	101	95-103
Plasma	103	97-107

Sensitivity

0.79ng/mL

Sequence

50:

MPSETPQAEV ; GPTGCPHRSG ; PHSAKGSLEK ; GSPEDKEAKE ; PLWIRPDAPS ;

100:

RCTWQLGRPA ; SESPHHHTAP ; AKSPKILPDI ; LKKIGDTPMV ; RINKIGKKFG ;

150:

LKCELLAKCE ; FFNAGGSVKD ; RISLRMIEDA ; ERDGTLKPGD ; TIIEPTSGNT ;

200:

GIGLALAAAV ; RGYRCIIVMP ; EKMSSEKVDV ; LRALGAEIVR ; TPTNARFDSP ;

250:

ESHVGVAWRL ; KNEIPNSHIL ; DQYRNASNPL ; AHYDTTADEI ; LQQCDGKLDM ;

300:

LVASVGTGGT ; ITGIARKLKE ; KCPGCRIIGV ; DPEGSILAEP ; EELNQTEQTT ;

350:

YEVEGIGYDF ; IPTVLDRTVV ; DKWFKSNDEE ; AFTFARMLIA ; QEGLLCGGSA ;

400:

GSTVAVAVKA ; AQELQEGQRC ; VVILPDSVRN ; YMTKFLSDRW ; MLQKGFLKEE ;

450:

DLTEKKPWWW ; HLRVQELGLS ; APLTVLPTIT ; CGHTIEILRE ; KGFDQAPVVD ;

500:

EAGVILGMVT ; LGNMLSSLLA ; GKVQPSDQVG ; KVIYKQFKQI ; RLTDTLGRLS ;

550:

HILEMDHFAL ; VVHEQIQYHS ; TGKSSQRQMV ; FGVVTAIDLL ; NFVAAQERDQ ;

551:

Sequence Length

551

String

P35520

Sub Unit

Homotetramer.

Uniprot	P35520
Gene Name	CBS
Immunogen Species	Homo Sapiens (Human)
Kegg	hsa:875
Unigene	Hs.533013

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SKU	Supplier No.	Size	Your price	Quantity
BHE13801836	E1957h	96T	$736		Add to Cart

ELISA Kit for Human Cystathionine beta-synthase

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