ELISA Kit for Human Caspase-8

Mfr.No.

E2015h

Alternative Names

Apoptotic cysteine protease ; Apoptotic protease Mch-5 ; CAP4 ; FADD-homologous ICE/ced-3-like protease ; FADD-like ICE ; FLICE ; ICE-like apoptotic protease 5 ; MORT1-associated ced-3 homolog ; MACH ; MCH5

Categories

Elisa

Function

Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp- ; -AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.

Specificity

Natural and recombinant Human Caspase-8

Subcellular Location

Cytoplasm

Detection Range

0.156-10ng/mL

Entryname

CASP8_HUMAN

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CASP8E and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	93-104%	92-106%	94-105%	95-103%
EDTA plasma(n=5)	94-102%	93-105%	96-101%	93-104%
heparin plasma(n=5)	90-105%	91-106%	92-104%	95-103%

Method Type

Sandwich

Platform

PF01335

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤7.6%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.7%

Protein Name

Caspase-8

Recovery

Recovery was determined by spiking various levels of CASP8 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	95-103
Plasma	98	94-102

Sensitivity

0.06ng/mL

Sequence

50:

MDFSRNLYDI ; GEQLDSEDLA ; SLKFLSLDYI ; PQRKQEPIKD ; ALMLFQRLQE ;

100:

KRMLEESNLS ; FLKELLFRIN ; RLDLLITYLN ; TRKEEMEREL ; QTPGRAQISA ;

150:

YRVMLYQISE ; EVSRSELRSF ; KFLLQEEISK ; CKLDDDMNLL ; DIFIEMEKRV ;

200:

ILGEGKLDIL ; KRVCAQINKS ; LLKIINDYEE ; FSKERSSSLE ; GSPDEFSNGE ;

250:

ELCGVMTISD ; SPREQDSESQ ; TLDKVYQMKS ; KPRGYCLIIN ; NHNFAKAREK ;

300:

VPKLHSIRDR ; NGTHLDAGAL ; TTTFEELHFE ; IKPHDDCTVE ; QIYEILKIYQ ;

350:

LMDHSNMDCF ; ICCILSHGDK ; GIIYGTDGQE ; APIYELTSQF ; TGLKCPSLAG ;

400:

KPKVFFIQAC ; QGDNYQKGIP ; VETDSEEQPY ; LEMDLSSPQT ; RYIPDEADFL ;

450:

LGMATVNNCV ; SYRNPAEGTW ; YIQSLCQSLR ; ERCPRGDDIL ; TILTEVNYEV ;

479:

SNKDDKKNMG ; KQMPQPTFTL ; RKKLVFPSD

Sequence Length

479

String

Q14790

Sub Unit

Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 10 kDa (p10) subunit. Interacts with FADD, CFLAR and PEA15. Isoform 9 interacts at the endoplasmic reticulum with a complex containing BCAP31, BAP29, BCL2 and/or BCL2L1. Interacts with TNFAIP8L2.

Uniprot	Q14790
Gene Name	CASP8
Immunogen Species	Homo Sapiens (Human)
Kegg	hsa:841
Unigene	Hs.599762

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BHE13802054	E2015h	96T	$736		Add to Cart

ELISA Kit for Human Caspase-8

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