ELISA Kit for Human Beta-nerve growth factor

Mfr.No.

E0105h

Alternative Names

NGFB

Categories

Elisa

Function

Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons.

Specificity

Natural and recombinant Human Beta-nerve growth factor

Subcellular Location

Secreted

Detection Range

15.6-1000pg/ml

Entryname

NGF_HUMAN

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NGF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	89-97%	92-102%	100-104%	91-98%
EDTA plasma(n=5)	90-98%	95-100%	90-96%	99-104%
heparin plasma(n=5)	95-102%	93-104%	97-102%	85-96%

Method Type

Sandwich

Platform

PF00243

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.5%

Protein Name

Beta-nerve growth factor

Recovery

Recovery was determined by spiking various levels of NGF into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	97	91-100
Plasma	98	95-106

Sensitivity

7.2pg/mL

Sequence

50:

MSMLFYTLIT ; AFLIGIQAEP ; HSESNVPAGH ; TIPQAHWTKL ; QHSLDTALRR ;

100:

ARSAPAAAIA ; ARVAGQTRNI ; TVDPRLFKKR ; RLRSPRVLFS ; TQPPREAADT ;

150:

QDLDFEVGGA ; APFNRTHRSK ; RSSSHPIFHR ; GEFSVCDSVS ; VWVGDKTTAT ;

200:

DIKGKEVMVL ; GEVNINNSVF ; KQYFFETKCR ; DPNPVDSGCR ; GIDSKHWNSY ;

241:

CTTTHTFVKA ; LTMDGKQAAW ; RFIRIDTACV ; CVLSRKAVRR ; A

Sequence Length

241

String

P01138

Sub Unit

Homodimer.

Uniprot	P01138
Gene Name	NGF
Immunogen Species	Homo Sapiens (Human)
Kegg	hsa:4803
Unigene	Hs.2561

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SKU	Supplier No.	Size	Your price	Quantity
BHE13808256	E0105h	96T	$536		Add to Cart

ELISA Kit for Human Beta-nerve growth factor

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