ELISA Kit for Human Annexin A5

Mfr.No.

E0259h

Antigen

ANXA5

Alternative Names

Anchorin CII ; Annexin V ; Annexin-5 ; Calphobindin I ; CBP-I ; Endonexin II ; Lipocortin V ; Placental anticoagulant protein 4 ; PP4 ; Placental anticoagulant protein I ; PAP-I ; Thromboplastin inhibitor ; Vascular anticoagulant-alpha ; VAC-alpha ; ANX5 ; ENX2 ; PP4

Categories

Elisa

Function

This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.

Specificity

Natural and recombinant Human Annexin A5

Detection Range

31.2-2000pg/mL

Entryname

ANXA5_HUMAN

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ANXA5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-102%	93-101%	91-103%	94-101%
EDTA plasma(n=5)	94-99%	93-100%	93-102%	96-99%
heparin plasma(n=5)	95-100%	92-103%	95-101%	95-102%

Method Type

Sandwich

Platform

PF00191

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤12.5%

Protein Name

Annexin A5

Recovery

Recovery was determined by spiking various levels of ANXA5 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	97	95-101
Plasma	98	93-102

Sensitivity

10pg/mL

Sequence

50:

MAQVLRGTVT ; DFPGFDERAD ; AETLRKAMKG ; LGTDEESILT ; LLTSRSNAQR ;

100:

QEISAAFKTL ; FGRDLLDDLK ; SELTGKFEKL ; IVALMKPSRL ; YDAYELKHAL ;

150:

KGAGTNEKVL ; TEIIASRTPE ; ELRAIKQVYE ; EEYGSSLEDD ; VVGDTSGYYQ ;

200:

RMLVVLLQAN ; RDPDAGIDEA ; QVEQDAQALF ; QAGELKWGTD ; EEKFITIFGT ;

250:

RSVSHLRKVF ; DKYMTISGFQ ; IEETIDRETS ; GNLEQLLLAV ; VKSIRSIPAY ;

300:

LAETLYYAMK ; GAGTDDHTLI ; RVMVSRSEID ; LFNIRKEFRK ; NFATSLYSMI ;

320:

KGDTSGDYKK ; ALLLLCGEDD ;

Sequence Length

320

String

P08758

Sub Unit

Monomer. Binds ATRX and EIF5B (By similarity). Interacts with hepatitis B virus (HBV).

Uniprot	P08758
Gene Name	ANXA5
Immunogen Species	Homo Sapiens (Human)
Kegg	hsa:308
Unigene	Hs.480653

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BHE13801081	E0259h	96T	$536		Add to Cart

ELISA Kit for Human Annexin A5

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