Aminopeptidases catalyze the hydrolysis of amino acid residues from the N-terminus of peptide or protein substrates. Able to cleave angiotensin III to generate angiotensin IV, a bioactive peptide of the renin-angiotensin pathway. Not able to cleave angiotensin I and angiotensin II. May play a role in the proteolytic processing of bioactive peptides in tissues such as testis and heart.
Specificity
Natural and recombinant Human Aminopeptidase O
Subcellular Location
Cytoplasm
Detection Range
78-5000pg/mL
Entryname
AMPO_HUMAN
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AMPO and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤6.3%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤9.4%
Protein Name
Aminopeptidase O
Recovery
Recovery was determined by spiking various levels of AMPO into serum and plasma .