ELISA Kit for Dog Tumor necrosis factor

Mfr.No.

E0133d

Alternative Names

Cachectin ; TNF-alpha ; Tumor necrosis factor ligand superfamily member 2 ; TNF-a ; TNFA ; TNFSF2

Categories

Elisa

Function

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.

Specificity

Natural and recombinant Dog Tumor necrosis factor

Subcellular Location

Tumor necrosis factor, soluble form Secreted

Detection Range

12.5-800pg/mL

Entryname

TNFA_CANFA

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	96-101%	96-105%	98-104%	98-107%
EDTA plasma(n=5)	95-103%	97-107%	100-110%	88-103%
heparin plasma(n=5)	98-103%	96-102%	93-104%	95-115%

Method Type

Sandwich

Platform

PF00229

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.2%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.4%

Protein Name

Tumor necrosis factor

Recovery

Recovery was determined by spiking various levels of TNF into serum and plasma .

Sample Type	Average(%)	Recovery Range (%)
Serum	102	95-114
Plasma	96	93-99

Sensitivity

6.25pg/mL

Sequence

50:

MSTESMIRDV ; ELAEEPLPKK ; AGGPPGSRRC ; FCLSLFSFLL ; VAGATTLFCL ;

100:

LHFGVIGPQR ; EELPNGLQLI ; SPLAQTVKSS ; SRTPSDKPVA ; HVVANPEAEG ;

150:

QLQWLSRRAN ; ALLANGVELT ; DNQLIVPSDG ; LYLIYSQVLF ; KGQGCPSTHV ;

200:

LLTHTISRFA ; VSYQTKVNLL ; SAIKSPCQRE ; TPEGTEAKPW ; YEPIYLGGVF ;

233:

QLEKGDRLSA ; EINLPNYLDF ; AESGQVYFGI ; IAL

Sequence Length

233

String

P51742

Sub Unit

Homotrimer.

Uniprot	P51742
Gene Name	TNF
Immunogen Species	Canis Lupus Familiaris (Dog)
Kegg	cfa:403922
Unigene	Cfa.54

Reference	1. "Tumour necrosis factor." Fiers W. [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] 2. "Cloning and expression of the cDNA for canine tumor necrosis factor-alpha in E. coli." Zucker K., Lu P., Fuller L., Asthana D., Esquenazi V., Miller J. Lymphokine Res.13:191-196(1994) [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] 3. "Genomic map of a portion of the canine MHC class I histocompatibility complex." Wagner J.L., Palti Y., DiDario D.D. Submitted (2003-09) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] 4. "Cytokine gene single nucleotide polymorphism (SNP) screening analyses in canine malignant histiocytosis." Soller J.T., Murua Escobar H., Janssen M., Fork M., Bullerdiek J., Nolte I. Submitted (2006-08) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] 5. "Expression of canine TNF, IL-1 and IL-6 mRNAs in peripheral blood monocytes and cell lines." Gilmore W.H., Carter S.D., Bennett M., Barnes A., Kelly D.F. Submitted (1996-03) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 74-205 strain: Beagle. tissue: Blood.

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BHE13811586	E0133d	96T	$900		Add to Cart

ELISA Kit for Dog Tumor necrosis factor

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