ELISA Kit for Dog Sex-determining region Y protein

Mfr.No.

E8883d

Alternative Names

Testis-determining factor ; TDF

Categories

Elisa

Function

Transcriptional regulator that controls a genetic switch in male development. It is necessary and sufficient for initiating male sex determination by directing the development of supporting cell precursors (pre-Sertoli cells) as Sertoli rather than granulosa cells. In male adult brain involved in the maintenance of motor functions of dopaminergic neurons. Involved in different aspects of gene regulation including promoter activation or repression. Promotes DNA bending. SRY HMG box recognizes DNA by partial intercalation in the minor groove. Also involved in pre-mRNA splicing. Binds to the DNA consensus sequence 5'-[AT]AACAA[AT]-3'.

Specificity

Natural and recombinant Dog Sex-determining region Y protein

Subcellular Location

Nucleus speckle Cytoplasm

Detection Range

0.312-20ng/ml

Entryname

SRY_CANFA

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SRY and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	93-103%	91-104%	90-107%	92-106%
EDTA plasma(n=5)	90-108%	95-102%	92-106%	94-105%
heparin plasma(n=5)	94-103%	90-107%	93-103%	91-108%

Method Type

Competitive

Platform

PF00505

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤6.7%

Protein Name

Sex-determining region Y protein

Recovery

Recovery was determined by spiking various levels of SRY into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	98	93-104
Plasma	99	92-105

Sensitivity

0.098ng/ml

Sequence

50:

MFRALNCDDH ; GAAVQQNAFG ; FPRKSSDRWT ; DNSTSNYRCE ; SGGNGRDSGR ;

100:

NRVRRPMNAF ; LVWSRDQRRK ; MALENPQMQN ; SEISKQLGYQ ; WKMLTEAEKW ;

150:

PFFEEAQRLQ ; AMHREKYPDY ; KYRPRRKATA ; QKSHKLLPAA ; SSSMLCKQVH ;

200:

VDERFYPFTY ; TDSCSRAAHT ; RMEDQLSCSQ ; PMSTARSLLQ ; QEYHSSSASL ;

220:

RDSPETLAAQ ; MSADASFYSK ;

Sequence Length

220

Sub Unit

Interacts with CALM, EP300, HDAC3, KPNB1, KRAB-O/ZFP748, PARP1, SLC9A3R2 and WT1.

Uniprot	Q9XT60
Gene Name	SRY
Immunogen Species	Canis Lupus Familiaris (Dog)

Reference	1. "Sry-negative XX sex reversal in purebred dogs." Meyers-Wallen V.N., Schlafer D., Barr I., Lovell-Badge R., Keyzner A. Mol. Reprod. Dev.53:266-273(1999) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]

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SKU	Supplier No.	Size	Your price	Quantity
BHE13810693	E8883d	96T	$900		Add to Cart

ELISA Kit for Dog Sex-determining region Y protein

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